Urine proteomes of healthy aging humans reveal extracellular matrix (ECM) alterations and immune system dysfunction.

Aging is a complex physiological process that poses considerable conundrums to rapidly aging societies. For example, the risk of dying from cardiovascular diseases and/or cancer steadily declines for people after their 60s, and other causes of death predominate for seniors older than 80 years of age. Thus, physiological aging presents numerous unanswered questions, particularly with regard to changing metabolic patterns. Urine proteomics analysis is becoming a non-invasive and reproducible diagnostic method. We investigated the urine proteomes in healthy elderly people to determine which metabolic processes were weakened or strengthened in aging humans. Urine samples from 37 healthy volunteers aged 19-90 years (19 men, 18 women) were analyzed for protein expression by liquid chromatography-tandem mass spectrometry. This generated a list of 19 proteins that were differentially expressed in different age groups (young, intermediate, and old age). In particular, the oldest group showed protein changes reflective of altered extracellular matrix turnover and declining immune function, in which changes corresponded to reported changes in cardiovascular tissue remodeling and immune disorders in the elderly. Thus, urinary proteome changes in the elderly appear to reflect the physiological processes of aging and are particularly clearly represented in the circulatory and immune systems. Detailed identification of "protein trails" creates a more global picture of metabolic changes that occur in the elderly.

[Predicting the risk of poor pregnancy outcome by ultrasound examination of yolk sac diameter]. / Ultrasonograficzna ocena pecherzyka zóltkowego w prognozowaniu ryzyka poronienia.

Ultrasound examination with 7.0 MHz transvaginal transducer was performed in 136 pregnant women between 5 and 12 weeks of pregnancy. Yolk Sac diameter and Crown-Rump Length were measured. It was observed that absence of YS or its diameter greater than 7 mm was usually associated with poor pregnancy outcome.

[Estimation of gasometry in umbilical cord blood and spirometry parameters at parturients in different positions]. / Ocena gazometrii krwi pepowinowej w zaleznosci od wartosci wybranych parametrów spirometrycznych u rodzacych w róznych pozycjach.

Estimation of umbilical cord blood gasometry is one of the test employed to postnatal state of newborns. This easy investigation can present the grade of newborns' blood oxygenation, which correlate with maternal lungs' ventilation. Because the spirometry parameters are more convenience in vertical position.

[Clinical evaluation of Zaditen syrup in the treatment of children with bronchial asthma]. / Kliniczna ocena skutecznosci preparatu Zaditen (syrop) u dzieci z dychawica oskrzelowa.

60 children with atopic asthma were included to the doubleblind study, in order to compare Zaditen and Clemastine. The trial was completed after 12 weeks of treatment. The efficacy was evaluated on the basis of clinical observation, lung function measurements and laboratory data. The Zaditen was shown to have more beneficial clinical effect on studied group than Clemastine. The lung function measurements before and after treatment did not showed much difference, although a significant decrease of the nonspecific bronchial reactivity to histamine was observed in the group receiving Zaditen . This was observed shortly after beginning of the treatment up to 6 weeks. Zaditen sirup was well tolerated and the only side effect observed was a significant weight increase, which could be explained by the elevation of serum sodium ions concentration.