ABSTRACT
The purpose of our research was to assess DNA-HPV frequention observation and evaluation of the diagnostic value mRNA E6 and E7 HPV16 and HPV18 profile concentration in prognostic risk of intraepithelial lesions and cervical cancer progression in women with cytological screening. Human papilloma virus (HPV) infection were detected in 13.8% normal samples, presence HPV16 and 18 in 7.5% samples were detected. HPV 16, 18 or HPV16 and 18 infection were detected in 85% HSIL (high-grade squamous intraepithelial lesion) samples. HPV16 or HPV18 infection in 100% cancer samples were detected. In samples from control group expression of E6 and E7 genes were not detected. In LSIL (low-grade squamous intraepithelial lesion) group HPV16 E7 gene in 2.6% samples, in HSIL group E7 gene in 9.5% samples were detected. In all cancer samples E7 or E6 HPV16 and/or HPV18 were detected.
Subject(s)
DNA, Viral/genetics , Papillomaviridae/genetics , Tumor Virus Infections/virology , Uterine Cervical Dysplasia/virology , Uterine Cervical Neoplasms/virology , Adult , Case-Control Studies , Cervix Uteri/virology , Female , Humans , Middle Aged , Oncogene Proteins, Viral , Poland , RNA, Messenger/genetics , Repressor Proteins , Reverse Transcriptase Polymerase Chain Reaction , Uterine Cervical Dysplasia/genetics , Uterine Cervical Dysplasia/prevention & control , Uterine Cervical Neoplasms/genetics , Uterine Cervical Neoplasms/prevention & controlABSTRACT
OBJECTIVES: The objective of the study was to estimate selected Fetal Pulmonary Artery blood flow parameters in pregnancies complicated by oligohydramnios in relation to normal pregnancies. MATERIALS AND METHODS: A total of 52 patients between 26 and 39 weeks of pregnancy complicated by oligohydramnios due to: placental insufficiency, PROM or idiopathic etiology and 69 normal pregnancies were involved in the study. Ultrasound examination using color and pulse Doppler were made. Signal was obtained from distal part of right or left pulmonary artery at the level of four chamber view. The RI, PI and S/D ratio were estimated. RESULTS: Significant increase in PI was observed in the group with severe oligohydramnios (AFI < 2.0 cm) compared to normal pregnancies. No significant differences were selected in RI and S/D ratio between mentioned groups. CONCLUSIONS: The study showed deterioration in fetal pulmonary artery blood flow in pregnancies complicated by severe oligohydramnios.
Subject(s)
Blood Flow Velocity , Oligohydramnios/diagnostic imaging , Pulmonary Artery/diagnostic imaging , Pulmonary Artery/embryology , Ultrasonography, Prenatal , Adult , Case-Control Studies , Female , Fetal Membranes, Premature Rupture/complications , Fetal Membranes, Premature Rupture/diagnostic imaging , Humans , Oligohydramnios/complications , Pregnancy , Risk Factors , Ultrasonography, Doppler, PulsedABSTRACT
Prospective studies ware carried in 200 lying-ins. To diagnose affective disorders medical interview and anonymous questionnaire BDI and EPDS were used. During interview 31% showed baby-blues. Signs of postpartum depression occurred in 18.5% women. No case of psychosis as well as critical incident of stress debriefing were stated. Recapitulating postpartum affective disorders occurred in 49.5% of examined group. Among negative psycho-socioeconomic factors pathological course of pregnancy in 14%, incorrect relationship with parents in 9%, low material status in 7%, unemployment in 32% and unwanted pregnancy in 3% of women were observed. Affective disorders in lying-ins women with postpartum depression are correlated with occurring of least 3 of above-mentioned factors. Baby-blues was found in 31%, while signs of depression were found in 17-20% of women during first week of puerperium. Existence of at least 3 negative psycho-socioeconomic factors during pregnancy or labour correlates with appearance of postpartum depression.