[The impact of acute poisoning by chlorfenvinphos on the activity of antioxidant enzymes and concentration of malondialdehyde in rats]. / Wplyw zatrucia ostrego chlorfenwinfosem na aktywnosc enzymów antyoksydacyjnych oraz stezenie dialdehydu malonowego u szczurów.

The aim of this paper was examination of the influence of chlorfenvinphos on the activity of an antioxidant enzymes in the blood and concentration of the serum malonondialdehyd in rats. It were found increase of the activity of SOD in 24 h and decrease in the 48 h; increase CAT activity, decrease GR activity in the 48 h and increase of G-6-P-DH activity in the 24 and 48 h after intoxication. Activity of GPX did not change statistically significant. It was observed decrease of MDA concentration in the serum in 1 and 24 h after intoxication and return to value in the control groups in the 48 h. It can be concluded that the changes of the activity of enzymes and concentration of MDA in the blood indicates hypoxia in the first period of intoxication (to 24 h) and reoxidation process in the later period.

[Changes in rats liver in late period of intoxication with chlorphenvinphos in a single dose]. / Zmiany w watrobie szczura w póznym okresie zatrucia chlorfenwinfosem w dawce jednorazowej.

The present investigation was undertaken to study the effect of chlorphenvinphos on the activity of liver enzymes in acute poisoning with this compound. The investigation was carried out on male Wistar rats. The animals received oil--control group and oil solution of chlorphenvinphos in dose 0.5 or 0.1 LD50--the examined group, intragastrically. Material for examination was collected in the 48 h after intoxication. Activity of GOT and GPT in a serum, cytosolic and mitochondrial fractions of liver; BGR and AcP in a serum and lysosomal fraction of liver, ChE in a serum and concentration of lactate in the cytosolic fraction of liver were assayed. It can be concluded that in the 48 h after intoxication with chlorphenvinphos, activity of ChE was normalized in the case of lower dose and has such tendency in the case of higher dose. The changes of other assayed parameters in the serum and liver homogenate indicates that in this period of study, the liver is in point of no return.

[Glycolytic processes in rat liver during chlorfenvinphos intoxications]. / Procesy glikolityczne w watrobie szczurów w zatruciu chlorfenwinfosem.

The aim of the study was to evaluate the activity of lactate dehydrogenase (LDH) and the concentration of lactate, pyruvate and glucose in cytosolic fraction of the rat liver in chlorfenvinphos intoxication. We found the increased activity of lactate dehydrogenase, decreased concentration of pyruvate with concurrent increase in the concentration of lactate and glucose in animal groups examined. The changes in the parameters investigated indicated the enhanced glycolytic process in the liver.

[Effect of pesticides on selected biochemical parameters in the serum and liver of rats]. / Wplyw pestycydów na wybrane parametry biochemiczne w surowicy i watrobie u szczurów.

The experiments were carried out on male Wistar rats, weighing 200-250 g. The animals received oil or oily solutions of carbaryl, parathion-methyl and IPO62 in doses of 0.5, 0.1 or 0.02 LD50. 1, 2, 4 or 24 h after the administration of pesticides, the activities of ChE and blood glucose level, liver glycogen level, FFA and TG concentrations in serum and TG level in liver were estimated. Comparing the enzymatic changes in acute intoxications with carbaryl, parathion-methyl or IPO62, in the same doses, it was found that mechanisms of efficacy of those compounds had different trends. Changes in the activities of such parameters as ChE in serum, BGR, AspAT and AlAT in serum and liver and blood glucose level depend on the dose of pesticides and this effect is observed for IPO62, parathion-methyl and carbaryl.

Blood glucose and insulin concentration in rats subjected to physical exercise in acute poisoning with parathion-methyl.

Unexercised rats or those subjected to single physical exercise were given parathion-methyl (10 mg/kg p.o. - 0.5 DL50) or oil (vehicle). Blood glucose and serum insulin concentration were assayed 1 h after the treatment. Physical exercise depressed the serum insulin concentration without changes in blood glucose level. In acute poisoning with parathion-methyl hyperglycemia occurred concurrently with hypoinsulinemia. Physical exercise abolished the pesticide-induced hypoinsulinemia, but not hyperglycemia.