Subject(s)
Accidents , CD4-CD8 Ratio/radiation effects , CD4-Positive T-Lymphocytes/radiation effects , Nuclear Reactors , Power Plants , T-Lymphocytes, Helper-Inducer/radiation effects , Adult , CD4-Positive T-Lymphocytes/ultrastructure , Dose-Response Relationship, Radiation , Flow Cytometry , Humans , Leukocyte Count/methods , Leukocyte Count/radiation effects , Male , Microscopy, Electron , T-Lymphocytes, Helper-Inducer/ultrastructure , Time Factors , UkraineABSTRACT
The flowing cytofluorometric and electron microscopic methods have been used to study the content and ultrastructure of blood CD4 lymphocytes in healthy and type I diabetic patients. Analysis of submicroscopic structure of the lymphocytes expressing CD4 antigen has revealed its morphological heterogeneity. It has permitted distinguishing four groups of cells. A decrease of the content of CD4+ lymphocytes at the expense of the first group cells and relative increase in the number of the fourth-group cells are observed in the majority of diabetic patients. The availability of parallel-tubular structures in granules forming clusters and first-group lymphocytes, an increase in the number and size of granules of the fourth-group are the most typical changes of ultrastructural characteristics.
Subject(s)
Diabetes Mellitus, Type 1/immunology , T-Lymphocytes, Helper-Inducer/immunology , CD4 Antigens/immunology , Flow Cytometry , Humans , Leukocyte Count , Microscopy, Electron , Reference Values , T-Lymphocytes, Helper-Inducer/ultrastructureABSTRACT
The content of lymphocytes of different immunological phenotypes: T11+ (all T-lymphocytes), T8+ (suppressors/killers), T4+ (inducers/helpers) and NKH1+ (natural killer cells) was investigated in the blood of 11 patients with type I diabetes mellitus by the method of flow-rate cytofluorimetry using a laser cell sorting device EPICS-C (Coulter), and a kit of monoclonal antibodies; 16 primary donors entered into the control group. As compared to the controls the patients demonstrated a statistically significant decrease in the absolute content of T11+, T4+, T8+ and especially NKH1+-cells. The results obtained confirmed previous data reported by us and other authors concerning disorder in the system of NK cells in insulin dependent diabetes mellitus. These results accounted for frequent development of infectious-inflammatory complications in this disease.
Subject(s)
Antibodies, Monoclonal , Diabetes Mellitus, Type 1/blood , T-Lymphocytes/classification , Cell Separation , Flow Cytometry , HumansABSTRACT
Carbostimulin is studied for the effect it exerts on indices of the acid-base equilibrium of blood, content of certain tricarboxylic-cycle metabolites and free amino acids and formation of antibodies in rats. It is established that carbostimulin feeding increases the total carbon dioxide, pyruvate and lactate concentration in blood, the content of ammonium, glutamine and certain free amino acids being decreased. Oxidation processes in the liver mitochondria and biosynthesis of antibodies in experimental animals are shown to be more intensive than in the control ones.
Subject(s)
Acid-Base Equilibrium/drug effects , Amino Acids/blood , Antibody Formation/drug effects , Carbonates/pharmacology , Citric Acid Cycle/drug effects , Magnesium Sulfate/pharmacology , Manganese Compounds , Manganese/pharmacology , Zinc Compounds , Zinc/pharmacology , Animals , Drug Combinations/pharmacology , Glycolysis/drug effects , Liver/metabolism , Male , Oxidation-Reduction , Rats , Rats, Inbred Strains , Tetanus Toxoid/immunologyABSTRACT
The paper is concerned with a study of the sodium bicarbonate, carboxylin and hydrochloric acid effect on certain indices of acid-base balance in blood and on dynamics of antibody formation in chickens. The sodium bicarbonate or carboxylin feeding increases the carbon dioxide total content and intensity of antipseudoplague antibodies biosynthesis in the blood by 12-21 or 12.5-40%, respectively, as compared with the control. The hydrochloric acid feeding causes a decrease in the total amount of CO2 in the blood and inhibition of antibody formation by 12.7-32.8 % as compared with the control.
Subject(s)
Acid-Base Equilibrium , Antibodies, Viral/biosynthesis , Carbon Dioxide/blood , Newcastle disease virus/immunology , Animals , Bicarbonates/blood , ChickensABSTRACT
The paper deals with dynamics of antibody formation in rats. Tetanic anatoxin independently of doses and methods of administration manifests weak immunogenic properties. A complete Freind adjuvant stimulates considerably the biosynthesis of antitetanic antibodies in the primary and secondary immune responses. Electrophoresis of tetanic anatoxin polyacrylamide gel permits isolating two protein fractions, one of them migrates in the immunoglobulin G zone and its mass constitutes 73%, the other-more low-molecular and contains 27% of protein. Dynamics of the acid-base blood state indices and of antibody biosynthesis is studied on models of chronic compensated alkalosis and metabolic acidosis in rats. It is established that during the experiment, simultaneously with an insignificant increase in the total carbonic acid concentration blood, there occurs a 25-60% increase in the titres of antitetanic antibodies as compared to the control. In rats with acidosis there occurs a simultaneously decrease in the intensity of antibody biosynthesis as compared to the control (43-54%) and in the total CO2 content in blood.
