The potentials of secondary metabolites from Bacillus cereus SN7 and Vagococcus fluvialis CT21 against fish pathogenic bacteria.

One of the major factors that affect the total production of fisheries is the declining number of catches and aquaculture production due to the high pathogenicity in aquatic environment. This enforces the need to find anti-pathogenic agents that could solve the problem. In addition, the application of potential Bacillus cereus SN7 and Vagococcus fluvialis CT21 isolated and identified from the sea water of Siak, Riau, Indonesia need to be optimally exploited. The aim of this study, therefore, is to determine the component of bioactive compounds present in Bacillus cereus SN7 and Vagococcus fluvialis CT21, and also to explore their intrinsic potential as a biological control agent in fisheries, especially for inhibiting the growth of pathogenic bacteria (Vibrio alginolyticus, Aeromonas hydrophila and Pseudomonas aeruginosa). The method used was experimental, where the ethyl acetate crude extracts of both samples were analyzed for their phytochemical content, followed by thin layer chromatography analysis and Liquid Chromatography Mass Spectrometry. In addition, anti-pathogenic activity test was performed using the Kirby-Bauer method, minimum inhibitory and bactericidal concentration analysis. The results showed alkaloids, flavonoids, and saponins were the potential bioactive components in the crude extracts of Bacillus cereus SN7 and Vagococcus fluvialis CT21. Furthermore, the anti-pathogenic activity test demonstrated the ability for both bacteria to inhibit three types of pathogens with the following inhibitory zone values: Vibrio alginolyticus (10-11 mm), Aeromonas hydrophila (8-12 mm), and Pseudomonas aeruginosa (8-10 mm). In conclusion secondary metabolite compounds produced by Bacillus cereus SN7 and Vagococcus fluvialis CT21 possess the capacity to inhibit pathogenic bacteria. Hence, both samples are potential candidates for anti-pathogen development, especially in fisheries.

Effect of different biofloc starters on ammonia, nitrate, and nitrite concentrations in the cultured tilapia Oreochromis niloticus system.

Background: High stocking density and intensive feeding in aquaculture systems lead to the accumulation of organic waste, which results in an increase in ammonia, nitrite, and nitrite concentrations in culture media. Biofloc is a potential technology to overcome this problem. The starter is a crucial carbon source for bacteria in the formation of biofloc. The objective of the present study aimed to explore the best starter of biofloc in a red tilapia Oreochromis niloticus culture system. Methods: A completely randomized design with four levels of treatment was used in this study. The tested starter was (A) control treatment, biofloc without starter, (B) biofloc with molasses starter, (C) biofloc with tapioca starter, and (D) biofloc with sucrose starter. The floc was cultured in 100-L tanks with a salinity of 17 ppt. The tanks were stocked with O.niloticus with a size of 3.71±0.11 cm at a stocking density of 30 fish per tank. The fish were fed on a commercial diet two times a day at satiation for 40 days. The ammonia, nitrite, and nitrite concentrations were measured for an interval of 8 days. Results: The study showed that the NH 3-N range was 0.02-0.07 mg L -1 (mean, 0.03 ± 0.02 mg L -1), NO 2-N range was 0.20-0.43 mg L -1 (mean, 0.25 ± 0.12 mg L -1), and NO 3-N range was 0.90-3.20 mg L -1 (mean, 1.42 ± 1.19 mg L -1). Conclusion: Among the starters tested, molasses was found to be the best for biofloc in tilapia culture.

Antibacterial activity in secondary metabolite extracts of heterotrophic bacteria against Vibrio alginolyticus, Aeromonas hydrophila, and Pseudomonas aeruginosa.

Background: Disease causing bacteria such as Vibrio alginolyticus, Aeromonas hydrophila, and Pseudomonas aeruginosa present a problem for fish farming. Treatment to remove them are generally carried out using antibiotics which have side effects on fish, the environment and humans. However, the use of antibacterial compounds derived from heterotrophic bacteria serve as a good alternative for antibiotics. Therefore, this study aimed to explore antibacterial activity in the secondary metabolite extracts of heterotrophic bacteria against Vibrio alginolyticus, Aeromonas hydrophila, and Pseudomonas aeruginosa. Methods: Heterotrophic bacteria namely Bacillus sp. JS04 MT102913.1, Bacillus toyonensis JS08 MT102920.1, Bacillus cereus JS10 MT102922.1, Bacillus sp. JS11 MT102923.1, Pseudoalteromonas sp. JS19 MT102924.1, Bacillus cereus JS22 MT102926.1, and Bacillus sp. strain JS25 MT102927.1 were used in this study. The sequences of these bacteria have been deposited and are available from NCBI GenBank. Each heterotrophic bacterium was cultured on 6L nutrient broth for 8 days, and extracts produced using ethyl acetate to obtain their secondary metabolites. These extracts were tested for their phytochemical contents using FT-IR and also tested for their inhibitory property in pathogenic bacteria by agar diffusion method. Results: Phytochemical test results showed that the seven heterotrophic bacterial isolates produced terpenoid compounds. Based on the inhibitory test, the secondary metabolite extracts from Bacillus sp strain JS04 had the highest inhibitory effect on the growth of pathogenic bacteria namely, V. alginolyticus (17.5 mm), A. hydrophila (16.8 mm), and P. aeruginosa (17.3 mm). Conclusion: It was concluded that the secondary metabolite extracts of heterotrophic bacteria inhibit the growth of V. alginolyticus, A. hydrophila, and P. aeruginosa.