Evaluation of the Oral Bacterial Genome and Metabolites in Patients with Wolfram Syndrome.

In Wolfram syndrome (WFS), due to the loss of wolframin function, there is increased ER stress and, as a result, progressive neurodegenerative disorders, accompanied by insulin-dependent diabetes. The aim of the study was to evaluate the oral microbiome and metabolome in WFS patients compared with patients with type 1 diabetes mellitus (T1DM) and controls. The buccal and gingival samples were collected from 12 WFS patients, 29 HbA1c-matched T1DM patients (p = 0.23), and 17 healthy individuals matched by age (p = 0.09) and gender (p = 0.91). The abundance of oral microbiota components was obtained by Illumina sequencing the 16S rRNA gene, and metabolite levels were measured by gas chromatography-mass spectrometry. Streptococcus (22.2%), Veillonella (12.1%), and Haemophilus (10.8%) were the most common bacteria in the WFS patients, while comparisons between groups showed significantly higher abundance of Olsenella, Dialister, Staphylococcus, Campylobacter, and Actinomyces in the WFS group (p < 0.001). An ROC curve (AUC = 0.861) was constructed for the three metabolites that best discriminated WFS from T1DM and controls (acetic acid, benzoic acid, and lactic acid). Selected oral microorganisms and metabolites that distinguish WFS patients from T1DM patients and healthy individuals may suggest their possible role in modulating neurodegeneration and serve as potential biomarkers and indicators of future therapeutic strategies.

Relationship between attention deficit hyperactivity disorder and temporomandibular disorders in adults: a questionnaire-based report.

OBJECTIVE: The symptoms of the attention deficit hyperactivity disorder (ADHD) persist in the adult years of life in most cases. They appear in adults with accompanying psychosocial problems. Temporomandibular disorder (TMD) refers to signs and symptoms associated with pain of non-dental origin in the oro-facial region, functional and structural disruptions of the masticatory system, especially the temporomandibular joints (TMJs) and masticatory muscles. The aim of the study was to show the relationship between the presence of ADHD symptoms in adulthood, in relation to the intensity of pain experienced in the face and problems connected to the TMD symptomatology, as well as sleep disorders. PATIENTS AND METHODS: The study group consisted of 252 individuals aged 18-55 years of both sexes, generally healthy. Each participant was asked to fill in several questionnaires, namely: ASRS (the World Health Organization ADHD Adult Self-Report Scale), DIVA (18 questions, 9 for concentration and attention disorders with an option in adulthood and childhood, 9 for hyperactivity and impulsivity with an option in adulthood and childhood), Athens Insomnia Scale, Stanford Sleepiness Scale (SSS), DC/TMD classification (Diagnostic Criteria for Temporomandibular Disorders - biaxial diagnostic criteria based on the biopsychosocial model). RESULTS: Results show that when ADHD symptoms observed in childhood persist, personality disorders, social relations disorders and affective disorders are found more often in adults than motor hyperactivity. CONCLUSIONS: There is a positive association between ADHD and the occurrence of symptoms of TMD in adults. This study confirmed this picture, extending it to include pain and sleep disorders.