Rare tradition of the folk medicinal use of Aconitum spp. is kept alive in Solcavsko, Slovenia.

BACKGROUND: Aconitum species are poisonous plants that have been used in Western medicine for centuries. In the nineteenth century, these plants were part of official and folk medicine in the Slovenian territory. According to current ethnobotanical studies, folk use of Aconitum species is rarely reported in Europe. The purpose of this study was to research the folk medicinal use of Aconitum species in Solcavsko, Slovenia; to collect recipes for the preparation of Aconitum spp., indications for use, and dosing; and to investigate whether the folk use of aconite was connected to poisoning incidents. METHODS: In Solcavsko, a remote alpine area in northern Slovenia, we performed semi-structured interviews with 19 informants in Solcavsko, 3 informants in Luce, and two retired physicians who worked in that area. Three samples of homemade ethanolic extracts were obtained from informants, and the concentration of aconitine was measured. In addition, four extracts were prepared according to reported recipes. RESULTS: All 22 informants knew of Aconitum spp. and their therapeutic use, and 5 of them provided a detailed description of the preparation and use of "voukuc", an ethanolic extract made from aconite roots. Seven informants were unable to describe the preparation in detail, since they knew of the extract only from the narration of others or they remembered it from childhood. Most likely, the roots of Aconitum tauricum and Aconitum napellus were used for the preparation of the extract, and the solvent was homemade spirits. Four informants kept the extract at home; two extracts were prepared recently (1998 and 2015). Three extracts were analyzed, and 2 contained aconitine. Informants reported many indications for the use of the extract; it was used internally and, in some cases, externally as well. The extract was also used in animals. The extract was measured in drops, but the number of drops differed among the informants. The informants reported nine poisonings with Aconitum spp., but none of them occurred as a result of medicinal use of the extract. CONCLUSIONS: In this study, we determined that folk knowledge of the medicinal use of Aconitum spp. is still present in Solcavsko, but Aconitum preparations are used only infrequently.

Folk use of medicinal plants in Karst and Gorjanci, Slovenia.

BACKGROUND: Information on the use of medicinal plants in Karst and Gorjanci is not available in the literature, but collection of plants is still an important and widespread practice in these regions. Karst and Gorjanci are two remote regions in Slovenia that are only 120 km apart but have different climates; one region is close to the Italian border, and the other is near the Croatian border. Our aim was to report and compare the use of medicinal plants in both regions. METHODS: From October 2013 to September 2014, 25 informants each in Karst and Gorjanci were interviewed during field research. The age of the informants ranged from 33 to 89 years, with an average age of 61 years in Karst and 69 years in Gorjanci. The main question was "Which plants do or did you collect from nature and use?" Plants of medicinal, nutritive, veterinary or cosmetic use were considered. RESULTS: A total of 78 and 82 taxa were reported in Karst and Gorjanci, respectively; 65 taxa were reported in both regions. Approximately 64% of the plants in each region were distinctive for only a few informants (fewer than 7). The remaining plants were considered important, and the majority were mutual to both regions. Few reported plants were typical for just one region. Differences in the use of some common medicinal plants were observed, e.g., Matricaria chamomilla was used mostly for the treatment of gastrointestinal disorders, respiratory infections and sore eyes in Gorjanci but as a calmative in Karst. Altogether, 15 different oral and 15 different topical medicinal preparations were reported. Folk knowledge was found to be influenced by the media, particularly popular books about medicinal plants that were published in the 20th century. CONCLUSIONS: The present research documents the folk use of medicinal plants in Karst and Gorjanci, Slovenia. This rapidly changing practice needs to be documented before it disappears or changes.

Catching flies with Amanita muscaria: traditional recipes from Slovenia and their efficacy in the extraction of ibotenic acid.

ETHNOPHARMACOLOGICAL RELEVANCE: Fly control is necessary for maintaining good hygiene on farms. Because organic farmers are skeptical about chemical pesticides, alternative fly control remedies are being considered. Amanita muscaria is a widespread fungus that contains ibotenic acid and muscimol. This fungus has been used to catch flies for centuries, but traditional recipes are poorly described, documented and characterized. AIM OF THE STUDY: The aim of the present study was to collect the traditional methods for preparing A. muscaria for catching flies in Karst and Gorjanci and to investigate the influence of different traditional methods on the release of ibotenic acid and muscimol from the fungal material. MATERIALS AND METHODS: The research was conducted in villages in Karst and in the foothills of Gorjanci, Slovenia. Data regarding the traditional recipes of A. muscaria for catching flies were collected through structured interviews with 31 people in Karst and 28 in Gorjanci. Eight preparations were prepared based on traditional methods, and the amount of ibotenic acid and muscimol released from the fungal material at five different time points (0.5, 1, 2, 3 and 24h) was determined by high-performance liquid chromatography (HPLC). RESULTS: Detailed descriptions of preparations used for catching flies were obtained from three informants in Karst, who were originally from other parts of Slovenia, and 13 informants in the foothills of Gorjanci. However, there were no reports regarding current usage. A total of 9 different methods were collected. Some methods were simple and included soaking in milk or water or dripping a little milk onto the mushroom. Others were more complex and included a combination of heat or mechanical processing and soaking in milk or water. For all preparations, the release of ibotenic acid was time-dependent, with the extracted amount increasing over time. Although milk was used more often than water in the traditional recipes, the release of both substances was not dependent on the solvent used. Fungal material that was exclusively soaked in water or milk released the smallest amount of ibotenic acid and muscimol at each time point. Additional heat and mechanical processing led to faster release of ibotenic acid and muscimol from the fungal material. CONCLUSIONS: The tradition of using A. muscaria for catching flies was present in Gorjanci but not in Karst. The methods used to prepare the fungal material vary, and these differences are reflected in the release profile of ibotenic acid.