Do birds select the plastics debris used for nest construction? A case study in a Mediterranean agricultural landscape.

Plastic pollution is becoming a global problem due to its ubiquitous occurrence and the impacts detected for many species. However, the research about plastics in nests of terrestrial bird species has remained relatively overlooked in comparison to those devoted to marine ecosystems. Here we study the occurrence and patterns of use of anthropogenic material in nests of two passerine birds, the Eurasian magpie (Pica pica) and the European serin (Serinus serinus), breeding in an orange tree cultivation in Mediterranean Spain. Our results show that both species use extensively plastic debris as nest material; almost 71% of the European serin nests and 96% of nests of Eurasian magpies contained plastic debris. Furthermore, by analyzing the plastic debris availability in the agricultural landscape surveyed we confirmed a selection pattern in the two species. Thus, both species preferably select plastic filaments over other plastic debris. The Eurasian magpie does not select plastic based on size or color but the European serin avoid black plastics prefer smaller fragments in comparison to the average size available. Moreover, we suggest the apparent similarity of plastic filaments with the natural materials typically used by these species, as well as how they use the plastic in their nests could influence their selection behavior. More studies focused on terrestrial birds inhabiting human modified habitats could offer a deeper approach to how plastic debris interacts with wildlife in different ways.

Correction: Sex, personality and conspecific density influence natal dispersal with lifetime fitness consequences in urban and rural burrowing owls.

[This corrects the article DOI: 10.1371/journal.pone.0226089.].

Nesting innovations in neotropical parrots associated to anthropogenic environmental changes.

Parrots are among the most diverse and widely distributed groups of birds and one of the most threatened bird orders mainly due to habitat loss and illegal poaching. Most parrots are obligate cavity nesters, so the logging of mature trees and the transformation of natural cliffs represent important threats to their conservation. Here, we report novel observations of Neotropical parrots nesting in previously unrecorded substrates. We show the first documented case of the cliff-nesting burrowing parrots trying to breed at ground level in an abandoned burrowing owl cavity. Additionally, we provide the first documented observations of this species attempting to nest in building cavities in three urbanized areas of Argentina. Moreover, we report data from four countries of 148 pairs of eight species typically breeding in tree cavity using palm tree bracts as nest sites. Behavioral plasticity in nest sites may allow parrots to maximize their nesting success by exploiting alternative breeding substrates. Moreover, these novelties could contribute to cope with habitat loss and further transformation. However, further research is needed to assess the consequences of these nesting innovations in terms of individual fitness and population dynamics as well as potential factors promoting their appearance.

Intraspecific competition and individual behaviour but not urbanization affect the dietary patterns of a generalist avian predator.

Urbanization has reshaped ecosystems and changed natural processes, driving an intense transformation of biomes, biotic community composition and diversity. Despite the growing interest in studying urban ecology over the last decades, the consequences of these changes on species occupying these ecosystems are not yet fully understood. Trophic generalism and tolerance to human disturbance have been proposed as two key traits in the colonization of urban environments. However, most studies focused on species' average traits, paying less attention to the potential role of inter-individual variability. Here, we examined diet specialization in urban and rural breeding pairs, as well as its relationship with individual behaviour and intraspecific competition, using the burrowing owl as a study model. Our results show that both urban and rural breeding pairs behaved as trophic specialists. The diet of burrowing owl breeding pairs followed a gradient from coleopteran- to micromammal-dominated, which is related to individual behaviour (bolder individuals consuming more coleopterans than shyer ones). Besides, pairs distant from others showed a more diverse diet than those experiencing higher levels of intraspecific competition. Models fitted separately for each habitat showed that the proportion of micromammals in the diet of urban breeding pairs was related to their behavior, while the diet of rural pairs was not affected by individual behavior but by intraspecific competition. However, despite the strong selection of tame and more explorative individuals in urban environments and the higher density they reach in this habitat type, they did not differ in their degree of diet specialization from rural conspecifics. Although it would be necessary to evaluate prey availability on a small scale, our results suggest that burrowing owl breeding pairs behave as specialists, despite the generalist character of the species, and that this specialization is not affected by the occupation of urban environments but to individual behaviour and intraspecific competition.

Social perception of mesocarnivores within hunting areas differs from actual species abundance.

Analyzing how similar social perception and ecological field data are might help identify potential biases in identifying and managing human-carnivore conflicts. We analyzed the degree of similarity between the perceived and field-measured relative abundance to unveil whether attitude towards carnivores of two groups of stakeholders, namely hunters and other local people, is underpinned or it is instead biased by alternative factors. Our results indicate that, in general, mesocarnivore perceived abundances were generally different to actual species abundance. We also found that the perceived abundance and attributed damage to small game species were related with respondents' ability to identify the carnivore species. We underline the existence of bias and the need to increase people knowledge on species distribution and ecological characteristics before adopting decisions when managing human-carnivore conflicts, especially for stakeholders that are directly involved in.

How Can We Improve Running Events? The IPA4RE Questionnaire and Its Potential Impact on the Experience of Runners.

The benefits that a positive running experience provides to individuals have been broadly studied by the scientific community, with the finding that running sport events are a facilitating tool to attract more individuals into physical activity. This study focuses on a sport management approach to improve the quality and organizational efficiency of running sports events so that a better experience for runners can be provided. The methods of this research consist of the validation of a new questionnaire called 'The IPA4RE questionnaire'. Furthermore, an IPA analysis for a specific event was carried out. As our main findings, the most valued aspects perceived by consumers are the event atmosphere, security, event route, and convenience of bib collection and event day information. In conclusion, the IPA4RE can be used as a management tool by running event organizers to make more efficient use of their resources and provide a better experience to participants.

Plastic Debris in Nests of Two Water Bird Species Breeding on Inland Saline Lakes in a Mediterranean Biosphere Reserve.

Despite more studies being carried out to know the impacts associated with plastic debris and much effort being spent on marine ecosystems, the impacts of plastics on terrestrial and freshwater species remain largely unknown. Here, we explored the presence of anthropogenic materials in nests of two wader species, the gull-billed tern (Gelochelidon nilotica) and the black-winged stilt (Himantopus himantopus), breeding on the inland salt lakes in the "La Mancha Húmeda" Biosphere Reserve, Central Spain. We revealed the presence of anthropogenic debris, mainly macroplastics (>5 mm), in 2.4% and 12.5% of the sampled nests of the gull-billed tern and the black-winged stilt, respectively. The fragments found in nests ranged from 8 mm to 257 mm for the gull-billed tern and from 7 mm to 19 mm for the black-winged stilt. This debris showed no clear pattern of color or size and probably originated both in the agricultural activities in the surroundings and domestic refuse. Although we did not detect any pernicious impacts on adults or chicks (e.g., entangled, injured, or dead individuals), the presence of plastics and other human waste directly placed in nests located in a protected area should warn us about the ubiquity of these pollutants, and the endocrine and immunological effects, among others, that may reduce the recruitment of new animals to the population should be assessed.

Drivers of the Ectoparasite Community and Co-Infection Patterns in Rural and Urban Burrowing Owls.

Urbanization creates new ecological conditions that can affect biodiversity at all levels, including the diversity and prevalence of parasites of species that may occupy these environments. However, few studies have compared bird-ectoparasite interactions between urban and rural individuals. Here, we analyze the ectoparasite community and co-infection patterns of urban and rural burrowing owls, Athene cunicularia, to assess the influence of host traits (i.e., sex, age, and weight), and environmental factors (i.e., number of conspecifics per nest, habitat type and aridity) on its composition. Ectoparasites of burrowing owls included two lice, one flea, and one mite. The overall prevalence for mites, lice and fleas was 1.75%, 8.76% and 3.50%, respectively. A clear pattern of co-infection was detected between mites and fleas and, to less extent, between mites and lice. Adult owls harbored fewer ectoparasites than nestlings, and adult females harbored more lice than males. Our results also show that mite and flea numbers were higher when more conspecifics cohabited the same burrow, while lice showed the opposite pattern. Rural individuals showed higher flea parasitism and lower mite parasitism than urban birds. Moreover, mite numbers were negatively correlated with aridity and host weight. Although the ectoparasitic load of burrowing owls appears to be influenced by individual age, sex, number of conspecifics per nest, and habitat characteristics, the pattern of co-infection found among ectoparasites could also be mediated by unexplored factors such as host immune response, which deserves further research.

Is exercise and electrostimulation effective in improving muscle strength and cardiorespiratory fitness in children with cystic fibrosis and mild-to-moderate pulmonary impairment?: Randomized controlled trial.

BACKGROUND: Evidence on resistance-training programs for cystic fibrosis (CF) is limited and the possible benefits of the addition of neuromuscular electrical stimulation (NMES) are unknown. This study aimed to evaluate the effects of a supervised resistance-training program, associated or not with NMES, on muscle strength, aerobic fitness, lung function and quality of life in children with CF presenting mild-to-moderate pulmonary impairment. METHODS: This is a randomized controlled trial including CF patients aged between 6 and 17years. Subjects were randomly allocated to control (CON); exercise (EX); or exercise and NMES (EX + NMES) groups, and evaluated at baseline and at the end of an 8-week individualized exercise-program (3 days/week, 60min/session). NMES was applied in the quadriceps and the interscapular region, simultaneously to the exercises. CON group followed the CF team recommendations. The main outcome measures were lung function, cardiorespiratory fitness, functional capacity, quality of life and muscle strength. RESULTS: Twenty-seven patients, aged 12.6 ± 3.0 years, were analyzed. No significant interactions were found for cardiorespiratory fitness. Functional capacity presented significant differences, indicating a better performance in both EX and EX + NMES. No significant changes between groups were seen for quality of life and lung function. As for muscle strength, EX and EX + NMES presented large effect sizes and significant differences, compared to CON, for quadriceps (p = 0.004, η2p = 0.401), pectoral (p = 0.001, η2p = 0.487), dorsal (p = 0.009, η2p = 0.333) and handgrip (p = 0.028, η2p = 0.278). CONCLUSION: A resistance exercise-training program led to improvements in muscle strength and functional capacity in CF patients with mild-to-moderate pulmonary impairment. The addition of NMES to the training program resulted in no extra favorable effects.

The New Generation of Professional Soccer Talent Is Born under the Bias of the RAE: Relative Age Effect in International Male Youth Soccer Championships.

In 2019, numerous championships of youth categories soccer national teams were held. In the present study, we analyzed the existence of the Relative Age Effect (RAE) in four major male championships that, due to their importance and participating teams, most likely brought together the great bulk of the players who will dominate professional soccer in the next decade. Participants were professional and amateur youth male soccer players who participated in the last international championships: UEFA European Under-21 Championship (2017-2019); UEFA European Under-19 Championship (2019); South American Youth Football Championship (also known as Conmebol U-20) (2019); and FIFA U-20 World Cup (2019), with 823 players (20.25 ± 0.84 years). In the four championships analyzed, the existence of RAE was found for all players (p < 0.001). Analyzing the players when considering their position on the pitch and their championship, RAE was found, statistically significant, in 10 of the 16 classifications. New generations of elite soccer players arrive with a clear bias in the selection of talent; an unfair bias, based on unequal opportunities in early categories, which should be reviewed by sports authorities.

The Role of Monk Parakeets as Nest-Site Facilitators in Their Native and Invaded Areas.

While most of the knowledge on invasive species focuses on their impacts, little is known about their potential positive effects on other species. Invasive ecosystem engineers can disrupt recipient environments; however, they may also facilitate access to novel resources for native species. The monk parakeet (Myiopsitta monachus) is a worldwide invader and the only parrot that builds its own communal nests, which can be used by other species. However, the ecological effects of these interspecific interactions are barely known. We compared the role of the monk parakeet as a nest-site facilitator in different rural and urban areas, both invaded and native, across three continents and eight breeding seasons. A total of 2690 nests from 42 tenant species, mostly cavity-nesting birds, were recorded in 26% of 2595 monk parakeet nests. Rural and invaded areas showed the highest abundance and richness of tenant species. Multispecies communal nests triggered interspecific aggression between the monk parakeet host and its tenants, but also a cooperative defense against predators. Despite the positive effects for native species, monk parakeets also facilitate nesting opportunities to other non-native species and may also transmit diseases to tenants, highlighting the complexity of biotic interactions in biological invasions.

The morphology of Colpocephalum pectinatum (Phthiraptera: Amblycera: Menoponidae) under scanning electron microscopy.

Here, we describe under scanning electron microscopy (SEM) the morphology of Colpocephalum pectinatum (Phthiraptera, Menoponidae), an ectoparasite found in burrowing owls, Athene cunicularia. We devote particular attention to the morphology of the main structures of the head (antennae and mouth-parts) and legs (tarsi and femoral ctenidia). Moreover, we describe the main peripheral sensory organs, located in the labial palpi and the distal end of antennae. We also detected that the structure of antennae and antennal sensilla arrangement are very similar to that described for other Colpocephalum and Menoponid species, and we discuss the function of each type of sensilla. We suggest that SEM studies combined with other microscopy and physiological techniques could be useful for elucidate the function of each structure, lice behaviour, as well as their taxonomy.

Effects of a Short-Term Resistance-Training Program on Heart Rate Variability in Children With Cystic Fibrosis-A Randomized Controlled Trial.

Background: Cystic fibrosis (CF) affects the autonomic nervous system (ANS) and exercise in healthy children modulates the interaction between sympathetic and parasympathetic activity. This study aimed to evaluate the effects of a short-term resistance exercise program on heart rate variability (HRV) in children and adolescents with CF. Methods: A randomized controlled trial was carried out in children diagnosed with CF aged 6-18 years. Individuals were divided into two groups: control (CON) and resistance-training (EX). Individuals in the EX group completed an individualized guided resistance program (5-RM-60-80%) for 8 weeks (3 sessions of 60 min/week). Upper and lower limbs exercises (seated bench press, seated lateral row, and leg press) were used. HRV was measured using a Suunto watch with subjects in lying position. Results: Nineteen subjects (13 boys) were included (CON = 11; and EX = 8). Mean age was 12.2 ± 3.3, FEV1 (forced expiratory volume in the first second) z-score was 1.72 ± 1.54 and peak oxygen consumption (VO2peak) 42.7 ± 7.4 mL.Kg-1.min-1. Exercise induced significant changes in the frequency-domain variables, including a decrease in LF power (p = 0.001, d = 0.98) and LF/HF ratio (p = 0.020, d = 0.92), and an increase in HF power (p = 0.001, d = -0.97), compared to the CON group. No significant changes were found for time-domain variables, although increases with a moderate effect size were seen for SDNN (p = 0.152, d = -0.41) and RMSSD (p = 0.059, d = -0.49) compared to the CON group. Conclusion: A short-term resistance exercise-training program was able to modulate HRV in children and adolescents with CF presenting mild to moderate lung function impairment and good physical condition. Clinical Trial Registration: www.ClinicalTrials.gov, identifier NCT04293926.

Urban life promotes delayed dispersal and family living in a non-social bird species.

In some vertebrate species, family units are typically formed when sexually mature individuals delay dispersal and independent breeding to remain as subordinates in a breeding group. This behaviour has been intensively studied in gregarious species but has also been described in non-social species where ecological and evolutionary drivers are less known. Here, we explore factors that favour delayed dispersal and family living and potential benefits associated with this strategy in a non-social, monogamous species (the burrowing owl, Athene cunicularia) occupying urban and rural habitats. Our results show that family units arise when first-year individuals, mainly males, delay their dispersal to stay in their natal nests with their parents. This delayed dispersal, while still uncommon, was more prevalent in urban (7%) than in rural (3%) habitats, and in areas with high conspecific density and productivity. Birds delaying dispersal contributed to the genetic pool of the offspring in 25% of the families analysed, but did not increase the productivity of the nests where they remained. However, their presence was related to an improvement in the body condition of chicks, which was ultimately linked to a slightly positive effect in offspring future survival probabilities. Finally, delayed dispersers were recruited as breeders in high-quality urban territories and closer to their natal nests than individuals dispersing during their first year of life. Thus, our results suggest that delaying dispersal may be mainly related to opportunities to inheriting a good quality territory, especially for males. Our study contributes to understanding the role played by habitat quality in promoting delayed dispersal and family living, not only in social but also non-social species, highlighting its impact in the ecology and evolution of animal populations.

Key performance indicators at FIFA Women's World Cup in different playing surfaces.

The aim of this study was to examine the key performance indicators of female professional soccer players during the 2011 and 2015 FIFA Women's World Cup, played on different surfaces (natural and artificial turf respectively). A total of 438 women from 24 national teams who participated at Canada 2015 (artificial turf) and 283 players from 16 national teams who played in Germany 2011 (natural grass) were selected for this study. The collected data were provided by OPTA Sports. Twenty-nine key performance indicators were included for analysis. The variables were calculated for the total sample and independently by positions (defense, midfielders and forwards) for matches on natural grass (2011) and artificial turf (2015). A Mann-Whitney U test was used out to identify differences between the sport surfaces. Moreover, a discriminant analysis was performed with the forced entry method to find the variables that better differentiated between the FIFA Women's World Cup 2011 (natural grass) and FIFA Women's World Cup 2015 (artificial turf). Key performance aspects were very similar between the two tournaments, but on natural grass, we observed a significantly higher number of total passes, successful dribbles, total tackles, successful tackles and interceptions. However, on artificial turf there were significantly higher percentages of success in total passes, and a higher number of fouls. This is an important factor for the choice of an elite competition surface because technical actions are crucial to the quality of the game and can influence the future behavior of spectators and fans.

Walkability and Fitness Center Prices, Opening Hours, and Extra Services: The Case of Madrid, Spain.

Walkability has been associated with urban development and political plans, contributing to more connected cities with improvements in communication, shopping, and pedestrian base. Among these services, fitness centers are becoming important elements for communities due to their impact on the health and welfare of citizens. The present study aims to examine how an area's Walk Score® affects fitness center services, specifically membership costs, opening hours, and aquatic services. Data from 193 fitness centers were retrieved, representing all the areas of the municipality of Madrid, Spain, including fitness centers in the 21 city districts. A nonlinear relationship between an area's Walk Score® and fitness centers' monthly fees is observed. Only in premium fitness centers, a weak curvilinear model is observed, following a quadratic equation, showing that fitness centers with higher prices are in less walkable areas. Additionally, the association between Walk Score® and a fitness center's opening hours reveals that fitness centers with wider hours of operation tend to be in moderately to highly walkable locations. Lastly, the existence of a swimming pool is related to a lower Walk Score®. Thus, fitness centers in less walkable areas try to offer additional services as differentiation from competitors, whereas centers in walkable locations use this advantage as a strength.

Are European Soccer Players Worth More If They Are Born Early in the Year? Relative Age Effect on Player Market Value.

The relative age effect (RAE) consists of the lower presence of members of an age group born in the months furthest from the age cut-off date established. In youth soccer, it is known that because of this effect the birth dates of more players in a team are closer to the cutoff of 1 January. These older players, due to their physical and psychological advantages, are more likely to be identified as talent. This study aimed to examine whether RAE can be identified in professional players of the top five European soccer leagues (Spain, Italy, England, Germany, and France) and to assess its influence on the perceived market value of the players. Market value data for 2577 players were obtained from the Transfermarkt database. A significant RAE was produced in all leagues (p < 0.05). However, this bias did not affect the market value of the professional elite soccer players examined here. Our observations indicate that, while the identification and promotion of talent at young ages are often biased by RAE, once players have reached the professional stage, the market value assigned to them is based more on factors other than their date of birth.

Sex, personality and conspecific density influence natal dispersal with lifetime fitness consequences in urban and rural burrowing owls.

There is a growing need to understand how species respond to habitat changes and the potential key role played by natal dispersal in population dynamics, structure and gene flow. However, few studies have explored differences in this process between conspecifics living in natural habitats and those inhabiting landscapes highly transformed by humans, such as cities. Here, we investigate how individual traits and social characteristics can influence the natal dispersal decisions of burrowing owls (Athene cunicularia) living in urban and rural areas, as well as the consequences in terms of reproductive success and apparent survival. We found short dispersal movements among individuals, with differences between urban and rural birds (i.e., the former covering shorter distances than the latter), maybe because of the higher conspecific density of urban compared to rural areas. Moreover, we found that urban and rural females as well as bold individuals (i.e., individuals with shorter flight initiation distance) exhibited longer dispersal distances than their counterparts. These dispersal decisions have effects on individual fitness. Individuals traveling longer distances increased their reproductive prospects (productivity during the first breeding attempt, and long term productivity). However, the apparent survival of females decreased when they dispersed farther from their natal territory. Although further research is needed to properly understand the ecological and evolutionary consequences of dispersal patterns in transformed habitats, our results provide information about the drivers and the consequences of the restricted natal movements of this species, which may explain its population structuring through restricted gene flow between and within urban and rural areas.

Characterization of an alkylresorcinol synthase that forms phenolics accumulating in the cuticular wax on various organs of rye (Secale cereale).

Alkylresorcinols are bioactive compounds produced in diverse plant species, with chemical structures combining an aliphatic hydrocarbon chain and an aromatic ring with characteristic hydroxyl substituents. Here, we aimed to isolate and characterize the enzyme that forms the alkylresorcinols accumulating in the cuticular wax on the surface of all above-ground organs of rye. Based on sequence homology with other type-III polyketide synthases, a candidate alkylresorcinol synthase was cloned. Yeast heterologous expression showed that the enzyme, ScARS, is highly specific for the formation of the aromatic resorcinol ring structure, through aldol condensation analogous to stilbene synthases. The enzyme accepts long-chain and very-long-chain acyl-CoA starter substrates, preferring saturated over unsaturated chains. It typically carries out three rounds of condensation with malonyl-CoA prior to cyclization, with only very minor activity for a fourth round of malonyl-CoA condensation and cyclization to 5-(2'-oxo)-alkylresorcinols or 5-(2'-hydroxy)-alkylresorcinols. Like other enzymes involved in cuticle formation, ScARS is localized to the endoplasmic reticulum. ScARS expression patterns were found correlated with alkylresorcinol accumulation during leaf development and across different rye organs. Overall, our results thus suggest that ScARS synthesizes the cuticular alkylresorcinols found on diverse rye organ surfaces.