ABSTRACT
El INER es una institución normativa en las afecciones respiratorias, quien a lo largo de su historia ha enfrentado desafíos ante situaciones emergentes de salud, como lo son la tuberculosis y el VIH. Actualmente afronta un nuevo reto de salud respiratoria, la epidemia de influenza A H1N1, la cual se presentó de forma inesperada en México en la primavera del 2009, provocando la implementación de medidas sanitarias urgentes, al rebasarse la capacidad de atención médica en el Instituto. Por ello, fue necesario organizar todos sus recursos humanos, físicos y materiales. Instaurar el Comité de Influenza Institucional y en apoyo y coordinación con el Comité de Control de Infecciones, tomar decisiones urgentes enfocadas a la prevención, manejo y control de la enfermedad. Se implementó el Triage en los servicios de concentración para la atención integral del paciente con sospecha y/o confirmación de influenza (Consulta Urgencias, Urgencias Observación, Unidades de Terapia Intensiva e Intermedia y clínico 5). Con todo ello y gracias a la intervención y el trabajo en equipo que realizó el profesional de enfermería del Instituto, se logró controlar la propagación de la epidemia y una vez más lograr conciencia en el personal de salud (médicos y enfermeras), para afrontar manera exitosa, nuevos retos en la salud respiratoria.
INER is a respiratory-illness treating institution which has faced diverse health challenges including tuberculosis and VIH; yet, at present, it is facing a new one: the unexpected 2009 A H1N1 influenza outbreak in Mexico. This massive incident provoked the implementation of urgent sanitary measurements including: the organization of human, physical and material resources; the establishment of-Infection Committee coordinated measurement towards illness prevention, control and management; and the establishment of the TRIAGE on concentration services towards patients with confirmed or dubious influenza (Urgencies, Consult, Urgencies Observation, Intensive Therapy Unit, Intermediate Therapy Unit and Clinical 5). Thanks to these measurements and the INER's nursing personnel team work, we were able to control the outbreak propagation, but also to create consciousness among doctors and nurses to successfully face the new respiratory-illnesses challenges.
Subject(s)
Humans , Male , Female , Nursing Care , Influenza, Human , Influenza A Virus, H1N1 SubtypeABSTRACT
An outbreak of myxomatosis occurred between September and October 1993 on a rabbit farm in Punta Colnett (Ensenada, Baja California in northwestern Mexico, Transpeninsular Highway, km 128) and was confirmed by the Mexico-USA Commission for Prevention of Foreign Diseases of Animals (CPA). This represents the first officially confirmed case of the disease in Mexico. Like the cases in California (USA), the brush rabbit (Sylvilagus bachmani) seems to be the carrier of the virus, since serum samples from wild rabbits from different areas of the peninsula of Baja California were found to contain antibodies against the myxoma virus.
Subject(s)
Disease Outbreaks/veterinary , Myxoma virus , Myxomatosis, Infectious/epidemiology , Rabbits , Tumor Virus Infections/veterinary , Animals , Animals, Domestic , Animals, Wild , Disease Reservoirs/veterinary , Insect Vectors/classification , Lagomorpha , Mexico/epidemiology , Myxoma virus/isolation & purification , Tumor Virus Infections/epidemiologyABSTRACT
The mdm2 gene encodes a zinc finger protein that negatively regulates p53 function by binding and masking the p53 transcriptional activation domain. Two different promoters control expression of mdm2, one of which is also transactivated by p53. We cloned and characterized the mdm2 gene from a murine 129 library. It contained at least 12 exons and spanned approximately 25 kb of DNA. Sequencing of the mdm2 gene revealed three nucleotide differences that resulted in amino acid substitutions in the previously published mdm2 sequence. Sequencing of normal BalbC/J DNA and the original cosmid clone isolated from the 3T3DM cell line revealed that they are identical, suggesting that the published sequence is in error at these three positions. In addition, we analyzed the expression pattern of mdm2 and found ubiquitous low-level expression throughout embryo development and in adult tissues. Analysis of mRNA from numerous tissues for several mdm2 spliced variants that had been identified in the transformed 3T3DM cell line revealed that these variants could not be detected in the developing embryo or in adult tissues.
Subject(s)
Nuclear Proteins , Proto-Oncogene Proteins/biosynthesis , Proto-Oncogene Proteins/genetics , 3T3 Cells , Animals , Antisense Elements (Genetics) , Base Sequence , Brain/embryology , Brain/metabolism , Cloning, Molecular , Cosmids , DNA Primers , Embryo, Mammalian , Exons , Ganglia, Spinal/embryology , Ganglia, Spinal/metabolism , Gene Expression , Genomic Library , In Situ Hybridization , Introns , Mice , Mice, Inbred BALB C , Molecular Sequence Data , Neoplasm Proteins/biosynthesis , Neoplasm Proteins/genetics , Polymerase Chain Reaction , Proto-Oncogene Proteins c-mdm2 , Restriction Mapping , Zinc FingersABSTRACT
Charcot-Marie-tooth disease type 1A (CMT1A) was localized by genetic mapping to a 3 cM interval on human chromosome 17p. DNA markers within this interval revealed a duplication that is completely linked and associated with CMT1A. The duplication was demonstrated in affected individuals by the presence of three alleles at a highly polymorphic locus, by dosage differences at RFLP alleles, and by two-color fluorescence in situ hybridization. Pulsed-field gel electrophoresis of genomic DNA from patients of different ethnic origins showed a novel SacII fragment of 500 kb associated with CMT1A. A severely affected CMT1A offspring from a mating between two affected individuals was demonstrated to have this duplication present on each chromosome 17. We have demonstrated that failure to recognize the molecular duplication can lead to misinterpretation of marker genotypes for affected individuals, identification of false recombinants, and incorrect localization of the disease locus.
Subject(s)
Charcot-Marie-Tooth Disease/genetics , Chromosomes, Human, Pair 17 , DNA Replication , Animals , Blotting, Southern , Chromosome Mapping , Cricetinae , Cricetulus , DNA/genetics , DNA/isolation & purification , Genes, Dominant , Genetic Linkage , Genetic Markers , Homozygote , Humans , Hybrid Cells/cytology , Mutation , Pedigree , Polymorphism, Restriction Fragment LengthABSTRACT
Hemos detectado una familia de tres generaciones a la que pertenecian cuatro miembros, fenotipicamente normales, y una nina propositus con retardo mental, todos ellos portadores de una inversion pericentrica en uno de los cromosomas numero 14; se les practico la tecnica de tincion con plata con el proposito de conocer si el cromosoma invertido tenia de tincion con plata con el proposito de conocer si el cromosoma invertido tenia actividad en sus NOR's, al mismo tiempo evaluar la frecuencia con la cual este cromosoma formaba asociacion NOR's. Se encontro que en todos los individuos este comosoma invertido fue positivo para la tincion de plata y el prosito presento la menor cantidad promedio de tincion asi como tambien la frecuencia de asociacion de NOR's. Igualmente se encontro una correlacion positiva entre el total del numero de cromosomas Ag (+) y el numero total de cromosomas asociados, asi como el porcentaje de asociacion del cromosoma invertido y su cantidad de tincion con plata, siendo el cromosoma anormal de la propositus el que presento las frecuencias mas bajas, esta observaciones sugieren una posible perdida de material cromosomico debido a una "aneusomie de recombinasio" durante el proceso de gametogenesis en su madre
