Nucleolus activity-dependent recruitment and biomolecular condensation by pH sensing.

DEAD-box ATPases are major regulators of biomolecular condensates and orchestrate diverse biochemical processes that are critical for the functioning of cells. How DEAD-box proteins are selectively recruited to their respective biomolecular condensates is unknown. We explored this in the context of the nucleolus and DEAD-box protein DDX21. We find that the pH of the nucleolus is intricately linked to the transcriptional activity of the organelle and facilitates the recruitment and condensation of DDX21. We identify an evolutionarily conserved feature of the C terminus of DDX21 responsible for nucleolar localization. This domain is essential for zebrafish development, and its intrinsically disordered and isoelectric properties are necessary and sufficient for the ability of DDX21 to respond to changes in pH and form condensates. Molecularly, the enzymatic activities of poly(ADP-ribose) polymerases contribute to maintaining the nucleolar pH and, consequently, DDX21 recruitment and nucleolar partitioning. These observations reveal an activity-dependent physicochemical mechanism for the selective recruitment of biochemical activities to biomolecular condensates.

The long non-coding RNA MALAT1 regulates intestine host-microbe interactions and polyposis.

The long non-coding RNA (lncRNA) Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) maintains the integrity of the intestinal epithelial barrier and regulates local inflammation. However, its influences on intestinal microbial communities and tissue susceptibility to cancer development remain unexplored. Here, we report that MALAT1 regulates host anti-microbial response gene expression and the composition of mucosal-associated microbial communities in a region-specific manner. In the APC mutant mouse model of intestine tumorigenesis, knocking out MALAT1 results in higher polyp counts in the small intestine and colon. Interestingly, intestine polyps that developed in the absence of MALAT1 were smaller in size. These findings highlight the unexpected bivalent role of MALAT1 in restricting and promoting cancer progression at different disease stages. Among the 30 MALAT1-targets shared by both the small intestine and colon, ZNF638 and SENP8 levels are predictive of colon adenoma patient overall survival and disease-free survival. Genomic assays further revealed that MALAT1 modulates intestinal target expression and splicing through both direct and indirect mechanisms. This study expands the role of lncRNAs in regulating intestine homeostasis, microbial communities, and cancer pathogenesis.

Low-loss 'crystalline-core/crystalline-clad' (C4) fibers for highly power scalable high efficiency fiber lasers.

We report the latest progress in fabrication and laser performance of the fully crystalline double-clad 'Yb:YAG-core/undoped-YAG-clad' fibers grown by the hybrid crystal growth method. The single-crystalline ytterbium (Yb) doped yttrium aluminum garnet (YAG) fiber cores were grown by the laser heated pedestal growth (LHPG) method, and the single-crystalline undoped YAG claddings were grown by the liquid phase epitaxy (LPE) technique, in which the single-crystalline Yb:YAG cores were used as the growth seeds. The key parameters of the hybrid-grown 'crystalline core/crystalline clad' (C4) fibers, including material composition, crystal structure, and fiber propagation loss, were characterized. The results confirmed that the grown C4 fibers, indeed, have both the single-crystalline fiber core and single-crystalline fiber clad. By utilizing a double-clad low-loss C4 fiber as a diode-cladding-pumped laser gain medium, we realized a fiber laser with the optical-to-optical conversion efficiency of 68.7% versus the incident pump power.

Single chip super broadband InGaN/GaN LED enabled by nanostructured substrate.

A new type of LED, single chip super broadband InGaN/GaN LED is presented in this paper. The LED is composed of an InGaN/GaN quantum well layer deposited on the nanostructured sapphire substrate, inscribed by femtosecond laser ablation. The super broadband emission is enabled due to the large variation of indium composition in a small local area so that different wavelengths can be emitted over a small area and the summation of these different emission wavelengths forms super broadband emission, which covers the entire visible spectral range. The result of this paper represents a major technological advance in white light LED lighting because it enables single chip white LED lighting without the need of phosphor down converter that can significantly improve the efficiency without the Stokes loss and reduce the cost.

Broadband supercontinuum generation covering UV to mid-IR region by using three pumping sources in single crystal sapphire fiber.

In this paper, we demonstrate that the the bandwidth of the supercontinuum spectrum generated in a large mode area sapphire fiber can be enhanced by employing triple pumping sources. Three pumping sources with wavelengths of 784 nm, 1290 nm, and 2000 nm are launched into a single crystal sapphire fiber that is 5 cm in length and has a core diameter of 115 microm. The nonlinear interactions due to self-phase modulation and four-wave mixing form a broadband supercontinuum that covers the UV, visible, near-IR and lower mid-IR regions. Furthermore, we explore the possibility of generating a broadband supercontinuum expanding from the UV to far-IR region by increasing the number of pumping sources with wavelengths in the mid- and far-IR.

Broadband IR supercontinuum generation using single crystal sapphire fibers.

In this paper, an investigation on broadband IR supercontinuum generation in single crystal sapphire fibers is presented. It is experimentally demonstrated that broadband IR supercontinuum spectrum (up to 3.2microm) can be achieved by launching ultra-short femtosecond laser pulses into single crystal sapphire fiber with a dimension 115microm in diameter and 5cm in length, which covers both the near IR spectral region and the lower end of the mid-IR spectral range. Furthermore, the mechanism of supercontinuum generation in single crystal sapphire fibers is briefly addressed. When the fiber length is shorter than the dispersion length, the self-phase modulation dominates the broadening effect. In this case, the broad supercontinuum spectrum with a smooth profile can be obtained. However, when the fiber length is longer than the dispersion length, the soliton-related dynamics accompanied by the self-phase modulation dominates the broadening effect. There are discrete spikes in the spectrum (corresponding to different order solitons). The above assumption of supercontinuum generation mechanism is quantitatively modeled by the computer simulation program and verified by the experimental results. Thus, one can adjust the spectral profile by properly choosing the length of the sapphire fibers. The broad IR spectral nature of this supercontinuum source can be very useful in a variety of applications such as broadband LADAR, remote sensing, and multi-spectrum free space communications.

Frequency division multiplexed multichannel high-speed fluorescence confocal microscope.

In this article, we report a new type of fluorescence confocal microscope: frequency division multiplexed multichannel fluorescence confocal microscope, in which we encode the spatial location information into the frequency domain. In this microscope, the exciting laser beam is first split into multiple beams and each beam is modulated at a different frequency. These multiple beams are focused at different locations of the target to form multiple focal points, which further generate multiple fluorescent emission spots. The fluorescent emissions from different focal points are also modulated at different frequencies, because the exciting beams are modulated at different frequencies (or difference carrier frequency). Then, all the fluorescent emissions (modulated at different frequencies) are collected together and detected by a highly sensitive, large-dynamic-range photomultiplier tube. By demodulating the detected signal (i.e., via the Fourier transform), we can distinguish the fluorescent light emitted from the different locations by the corresponding carrier frequencies. The major advantage of this unique fluorescence confocal microscope is that it not only has a high sensitivity because of the use of photomultiplier tube but also can get multiple-point data simultaneously, which is crucial to study the dynamic behavior of many biological process. As an initial step, to verify the feasibility of the proposed multichannel confocal microscope, we have developed a two-channel confocal fluorescence microscope and applied it to study the dynamic behavior of the changes of the calcium ion concentration during the single cardiac myocyte contraction. Our preliminary experimental results demonstrated that we could indeed realize multichannel confocal fluorescence microscopy by utilizing the frequency division multiplexed microscope, which could become an effective tool to study the dynamic behavior of many biological processes.