ABSTRACT
A novel bacterial strain, designated THYL-44(T), was isolated from the soil of a Euphrates poplar (Populus euphratica) forest in Xinjiang, China. The cells were strictly aerobic, Gram-staining-negative, non-flagellated, non-motile and filamentous. Growth occurred at 17-37 degrees C (optimum 30 degrees C), at pH 5.0-8.0 (optimum pH 7.0) and with 0-1 % NaCl (w/v; optimum 0 %). Flexirubin pigments were not produced. A phylogenetic analysis based on 16S rRNA gene sequences revealed that strain THYL-44(T) was closely related to Niastella koreensis KACC 11465(T) (95.5 % sequence similarity). The major respiratory quinone was MK-7 and the predominant cellular fatty acids were iso-C(15 : 0) (28.6 %), iso-C(17 : 0) 3-OH (23.9 %) and iso-C(15 : 1) G (17.4 %). The DNA G+C content was 45.2 mol%. Therefore, the phylogenetic, physiological and chemotaxonomic data demonstrated that strain THYL-44(T) represents a novel species of the genus Niastella , for which the name Niastella populi sp. nov. is proposed. The type strain is THYL-44(T) (=CCTCC AB 208238(T)=KCTC 22560(T)). On the basis of new data, an emended description of the genus Niastella is also proposed.
Subject(s)
Populus/microbiology , Soil Microbiology , Sphingobacterium/classification , Sphingobacterium/isolation & purification , DNA, Bacterial/genetics , DNA, Ribosomal/genetics , Fatty Acids/chemistry , Fatty Acids/metabolism , Molecular Sequence Data , Phylogeny , RNA, Ribosomal, 16S/genetics , Sphingobacterium/genetics , Sphingobacterium/metabolismABSTRACT
A novel golden-yellow-coloured strain, designated RHYL-37(T), was isolated from a soil sample from a forest of Euphrates poplar (Populus euphratica) in Xinjiang, China. The cells were strictly aerobic, Gram-staining-negative, heterotrophic, non-spore-forming rods that were motile by gliding. Growth occurred at 17-37 degrees C (optimum, 30 degrees C), at pH 5.0-9.0 (optimum, pH 7.0) and with 0-1 % NaCl (w/v; optimum, 0 %). Phylogenetic analysis based on 16S rRNA gene sequences indicated that the novel isolate formed a cluster with members of the genera Segetibacter, Terrimonas, Niastella, Niabella and Chitinophaga in the family 'Chitinophagaceae' in the phylum Bacteroidetes. Sequence similarities with the type strains of recognized species ranged from 90.6 to 94.0 %. The predominant isoprenoid quinone was MK-7 and the major cellular fatty acids were iso-C(15 : 0), iso-C(15 : 1), C(16 : 0) and iso-C(17 : 0) 3-OH. The DNA G+C content was 39.7 mol%. On the basis of the phylogenetic, physiological and chemotaxonomic data, strain RHYL-37(T) represents a novel genus and species in the family 'Chitinophagaceae', for which the name Parasegetibacter luojiensis gen. nov., sp. nov. is proposed. The type strain of Parasegetibacter luojiensis is RHYL-37(T) (=CCTCC AB 208240(T)=KCTC 22561(T)).
