ABSTRACT
OBJECTIVE: To study the volatile constituents from peel of Aquilaria sinensis (Lour.) Gilg. and its anti-tumor activity. METHODS: The volatile components from peel of A. sinensis were extracted by chloroform and analysed by the GC-MS technique. Assay the peel extract of A. sinensis on MCF-7 cell proliferation using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) method. RESULTS: GC-MS gave 131 peaks and 28 compounds have been identified. The contents of the benzene compounds accounted 7.79% of the volatile components, the esquiterpenes accounted 5.44%, and the relative content of fatty acids accounted 3.08%. In addition, two 2-(2-phenylethyl)-color ketones and a chromone component were detected, and the total relative content accounted 12.3%. The inhibition rate of human breast cancer cells reached 99. 6% when the concentration was 500 microg/mL. CONCLUSION: The volatile components from peel of A. sinensis were analysed and a large number of characteristic elements of A. sinensis were detected for the first time. The chloroform extract from peel of A. sinensis significantly inhibited the proliferation of human breast cancer cells.
Subject(s)
Antineoplastic Agents, Phytogenic/pharmacology , Benzylammonium Compounds/analysis , Oils, Volatile/analysis , Sesquiterpenes/analysis , Thymelaeaceae/chemistry , Antineoplastic Agents, Phytogenic/isolation & purification , Benzylammonium Compounds/pharmacology , Breast Neoplasms/pathology , Cell Line, Tumor , Cell Proliferation/drug effects , Chromones/analysis , Chromones/pharmacology , Fatty Acids/analysis , Fatty Acids/pharmacology , Female , Fruit/chemistry , Gas Chromatography-Mass Spectrometry/methods , Humans , Oils, Volatile/chemistry , Oils, Volatile/pharmacology , Sesquiterpenes/pharmacologyABSTRACT
OBJECTIVE: To study the difference of rDNA ITS sequences between Cirtus grandis var. tomentosa and its relatives. METHODS: The rDNA ITS gene fragment was amplified by PCR with universal primers of rDNA ITS and sequenced. RESULTS: The DNA sequences of ITS ranged from 675 to 683 bp and the 5.8S gene of all of the samples are 165 bp in size. Distance values of ITS sequence, estimated according to Kimura two-parameter models between C. grandis var. tomentosa and C. grandis var. shatin-you, C. grandis var. tomentosa and C. reticulata were 1.05 and 2.29 respectively. CONCLUSION: There are some differences in rDNA ITS sequences among C. grandis var. tomentosa and its relatives.
