ABSTRACT
Rhodopseudomonas palustris strain JSC-3b isolated from a water canal adjacent to a vegetable field produces a protein that was purified by bioactivity-guided fractionation based on ammonium sulfate precipitation, ion-exchange absorption and size exclusion. The protein was further identified as an endoribonuclease L-PSP (Liver-Perchloric acid-soluble protein) by shotgun mass spectrometry analysis and gene identification, and it is member of YER057c/YjgF/UK114 protein family. Herein, this protein is designated Rhp-PSP. Rhp-PSP exhibited significant inhibitory activities against tobacco mosaic virus (TMV) in vivo and in vitro. To our knowledge, this represents the first report on the antiviral activity of a protein of the YER057c/YjgF/UK114 family and also the first antiviral protein isolated from R. palustris. Our research provides insight into the potential of photosynthetic bacterial resources in biological control of plant virus diseases and sustainable agriculture.
Subject(s)
Antiviral Agents/chemistry , Bacterial Proteins/chemistry , Rhodopseudomonas/metabolism , Amino Acid Sequence , Antiviral Agents/isolation & purification , Antiviral Agents/pharmacology , Bacterial Proteins/genetics , Bacterial Proteins/pharmacology , Chromatography, High Pressure Liquid , Molecular Sequence Data , Plant Leaves/virology , Sequence Alignment , Tandem Mass Spectrometry , Nicotiana/growth & development , Nicotiana/virology , Tobacco Mosaic Virus/drug effects , Tobacco Mosaic Virus/isolation & purificationABSTRACT
OBJECTIVE: This study aimed to isolate, identify and clone degrading gene of a synthetic pyrethroids degrading bacterium. METHODS: A photosynthetic bacterial strain PSB07-21 capable of degrading several synthetic pyrethroids efficiently was isolated by an enrichment culture. PSB07-21 was identified based on its morphology, physiology and phylogenetic analysis of 16S rDNA sequence. The degradation ability of this strain was evaluated with gas chromatography.The degrading gene was cloned with PCR. RESULTS: PSB07-21 was closely related to Rhodopseudomonas sp. The optimum condition of degrading synthetic pyrethroidss was at 35 degrees C, pH 7 and 3000 lx. PSB07-21 could degrade fenpropathrin, cypermethrin and bipthenthrin by 66.63%, 43.25% and 50.18% in a concentration of 600 mg/L at day 15, respectively. We cloned a putative gene which was 326bp long with 37.0% identical to 20G-Fe (II) oxygenase gene. When compensating low concentration Fe (II) in PSB medium with synthetic pyrethroids, the degradation efficiency of PSB07-21 was enhanced. CONCLUSION: The strain has the potential application to synthetic pyrethroids bioremediation.
Subject(s)
Bacterial Proteins/genetics , Cloning, Molecular , Pyrethrins/metabolism , Rhodopseudomonas/isolation & purification , Rhodopseudomonas/metabolism , Bacterial Proteins/metabolism , Biodegradation, Environmental , Molecular Sequence Data , Phylogeny , Rhodopseudomonas/classification , Rhodopseudomonas/geneticsABSTRACT
Three control measures of Ambrosia artemisiifolia, including biological control, chemical control, and CK without any treatment, were evaluated by analytic hierarchy process (AHP). Corresponding contributions of the three control measures to comprehensive profit (CP) and comprehensive cost (CC) were calculated and ranked, which were regarded as the assessment criteria of the control measures. The results showed that among the three control measures, biological control had the highest CP and the lowest CC, CK was in adverse; and chemical control was in intervenient. Biological control had the highest ratio of profit to cost, and suggesting that this control measure is an optimal and recommendable measure in controlling A. artemisiifolia.
