ABSTRACT
Endothelial NO-synthase (eNOS) gene allelic polymorphism in 221 patients with acute coronary syndrome and in 83 practically healthy people was determined. It was shown that interrelations of normal homozygotes, heterozygotes and pathologic homozygotes in T/C promoter polymorphism analysis accout 48%, 36% and 16% correspondingly (in control--48%, 46%, 6%; P < 0.05 by chi2-test); in G894 --> T polymorphism ofexon 7 analysis--34%, 58%, 8% (in control--29%, 67%, 4%; P > 0.05), and in determination of 4a/4b polymorphism of intron 4--64.5%, 31% and 4.5% (in control--62.5%, 32.5%, 5%; P > 0.05). Obtained data show that eNOS C/C promoter variant is a risk factor of acute coronary syndrome in Ukrainian population.
Subject(s)
Coronary Disease/genetics , Gene Frequency/genetics , Genetic Predisposition to Disease , Nitric Oxide Synthase/genetics , Polymorphism, Restriction Fragment Length , Promoter Regions, Genetic , Acute Disease , Adult , Aged , Aged, 80 and over , Coronary Disease/enzymology , Coronary Disease/epidemiology , Exons , Female , Genotype , Humans , Introns , Male , Middle Aged , Nitric Oxide Synthase Type III , Polymerase Chain Reaction , Risk Factors , Syndrome , Ukraine/epidemiologyABSTRACT
Frequency of promoter endothelial NO-synthase gene allelic polymorphism by using polymerase chain reaction and restriction fragment length polymorphism (RFLP-PCR) was determined in 221 patients with acute coronary syndrome (ACS) and in 83 almost healthy subjects. Data obtained indicate that different promoter allelic variant frequency differs significantly in patients with ACS and in control group. Correlation of normal homozygotes (T/T), heterozygotes (T/C) and pathologic homozygotes (C/C) was 48%, 36% and 16% respectively in patients, and in control it was 48%, 46%, 6% (P<0.05 by c2-test). Thus, in patients with ACS in Ukrainian population pathologic C/C variants of 5'-flanking region of eNOS gene were found 2.7-times more often in ACS patients, than in control. This allows us to suggest, that this allelic polymorphism can be considered as one of genetic risk factors of ACS development.
