The accumulation of adversity in midlife: Effects on depressive symptoms, life satisfaction, and character strengths.

Middle-aged adults are a central pillar of society because they comprise large segments of the workforce and bridge younger and older generations. Given the significant role that middle-aged adults play for the greater good of society, more research is warranted to evaluate in which ways adversity could accumulate or pile-up to impact pertinent outcomes. We used data from a sample of middle-aged adults (n = 317, age 50-65 at baseline, 55% women) who were assessed monthly for a period of 2 years to examine whether the accumulation of adversity was predictive of depressive symptoms, life satisfaction, and character strengths (generativity, gratitude, the presence of meaning, and search for meaning). Greater accumulation of adversity was associated with reporting more depressive symptoms, lower life satisfaction, and the lower presence of meaning and the effects remained for depressive symptoms when accounting for concurrent adversity. More concurrent adversity was associated with reporting more depressive symptoms and lower life satisfaction and lower levels of generativity, gratitude, and the presence of meaning. Analyses that targeted specific domains of adversity showed that the accumulation of adversity associated with close family members (i.e., spouse/partner, children, and parents), and financial, and work domains showed the strongest (negative) associations across each outcome. Our findings demonstrate that monthly adversity take its toll on pertinent midlife outcomes and points to future research targeting mechanisms underlying our findings as well as resources that promote positive outcomes. (PsycInfo Database Record (c) 2023 APA, all rights reserved).

Digital media use frequency, online behaviors, and risk for negative mental health in adolescents in high-achieving schools.

Replicated evidence shows that adolescents enrolled in high-achieving schools exhibit elevated mental health problems relative to national norms, reflecting risk factors such as achievement and social pressures. The frequency of digital media use is similarly a potential risk factor for poor youth mental health, although mediators of this association have not been identified. 2952 youth from three high-achieving U.S. high schools reported the frequency of their digital media use as well as internalizing and externalizing problems and substance use. Using a multiple mediation framework, the frequency of social comparison, receiving negative feedback, and risky self-presentation online each uniquely mediated the association of digital media use with internalizing and externalizing problems in boys and girls; for substance use, risky self-presentation mediated this association in both boys and girls and negative feedback mediated substance use in girls only. Measurable online behaviors in the form of social comparison, negative feedback, and self-presentation may crucially underlie the association of digital media use frequency with socio-emotional development in adolescents. Implications for intervention focused on impacting online behaviors for improving youth mental health are discussed.

"More is better" or "better near the middle"? A U.S.-based individual participant data meta-analysis of socioeconomic status and depressive symptoms.

Socioeconomic status (SES) is a widely researched construct in developmental science, yet less is known concerning relations between SES and adaptive behavior. Specifically, is the relation linear, with higher SES associated with better outcomes, or does the direction of association change at different levels of SES? Our aim was to examine linear ("more is better") and quadratic ("better near the middle") associations between components of SES (i.e., income, years of education, occupational status/prestige) and depressive symptoms (Center for Epidemiologic Studies-Depression Scale), and to explore moderation by developmental period (adolescence, young, middle, and older adulthood), gender/sex (female, male), and race/ethnicity (Asian American, Black, Latinx, multiracial, Native American, White). We hypothesized that there would be more support for a model containing quadratic associations. We conducted a two-stage meta-analytic structural equation model of 60 data sets (27,242 correlations, 498,179 participants) within the United States, accounting for dependencies between correlations, which were identified via the Interuniversity Consortium for Political and Social Research and handled using a two-step approach. Income was quadratically associated with depressive symptoms, but the quadratic model did not explain more variance in depressive symptoms than the linear model. Developmental period and race/ethnicity moderated the associations: Income was quadratically associated with depressive symptoms among middle-aged adults, and years of education were quadratically associated with depressive symptoms among White samples. Our findings suggest that researchers and clinical practitioners should consider the elevated risk of depressive symptoms for individuals from low and high-income backgrounds in the United States. (PsycInfo Database Record (c) 2023 APA, all rights reserved).

Socioeconomic status and depressive symptoms: An individual-participant data meta-analysis on range restriction and measurement in the United States.

INTRODUCTION: The association between socioeconomic status (SES) and depressive symptoms is well documented, yet less attention has been paid to the methodological factors contributing to between-study variability. We examined the moderating role of range restriction and the depressive-symptom measurement instrument used in estimating the correlation between components of SES and depressive symptoms. METHODS: We conducted an individual participant data meta-analysis of nationally-representative, public-access datasets in the United States. We identified 123 individual datasets with a total of 1,655,991 participants (56.8 % female, mean age = 40.33). RESULTS: The presence of range restriction was associated with larger correlations between income and depressive symptoms and with smaller correlations between years of education and depressive symptoms. The measurement instrument of depressive symptoms moderated the association for income, years of education, and occupational status/prestige. The Center for Epidemiological Studies-Depression scale consistently produced larger correlations. Higher measurement reliability was also associated with larger correlations. LIMITATIONS: This study was not a comprehensive review of all measurement instruments of depressive symptoms, focused on datasets from the United States, and did not examine the moderating role of sample characteristics. DISCUSSION: Methodological characteristics, including range restriction of SES and instrument of depressive symptoms, meaningfully influence the observed magnitude of association between SES and depressive symptoms. Clinicians and researchers designing future studies should consider which instrument of depressive symptoms is suitable for their purpose and population.

A Systematic Overview of Meta-Analyses on Socioeconomic Status, Cognitive Ability, and Achievement: The Need to Focus on Specific Pathways.

Meta-analyses on the relation between socioeconomic status (SES) and performance on measures of cognitive ability and achievement arrive at the same general conclusion of a small to medium association. Advancements in methods make possible for meta-analyses to examine specific pathways linking SES to cognitive ability and achievement, as well as the moderators of these pathways. In this study, we conducted a systematic overview of meta-analyses on SES to address three research questions: 1) what is the direction and overall strength of association between SES and performance on measures of cognitive ability and achievement, and how precise are the effect sizes reported? 2) to what extent have meta-analyses examined moderation by components of SES, age, sex, and race/ethnicity? and 3) to what extent have meta-analyses examined mechanisms linking SES to cognitive ability and achievement? We conducted a systematic search using online archives (i.e., PsycINFO, ERIC, PubMed, Sociological Abstracts, and Web of Science), searching issues in Psychological Bulletin and Review of Educational Research, and examining references and citations. We identified 14 meta-analyses published between 1982 and 2019. These meta-analyses consistently reported positive associations of small to medium magnitude, indicating that SES is a meaningful contributor to the development of cognitive ability and achievement. Fewer meta-analyses reported evidence of moderation by age, sex, and race/ethnicity. None of the meta-analyses directly examined mechanisms, but provided evidence of possible mechanisms for future research. We suggest that meta-analyses can increase their contribution to future research, interventions, and policy by narrowing their focus on specific pathways.

Lifetime Adversity in the Context of Monthly Adversity and Psychological Well-Being in Midlife: Evidence of Cumulative Disadvantage, But Not Steeling Effects of Lifetime Adversity.

OBJECTIVES: Our objective is to examine whether lifetime adversity has either a "steeling effect" or "cumulative disadvantage effect" on the consequences of monthly adversity on psychological well-being in middle-aged adults. An exploratory step was to examine whether such associations differed based on the domain of adversity (personal, family/friend, bereavement, social-environmental, and relationship). METHOD: Multilevel modeling was applied to data from a sample of participants in midlife (n = 358, ages 50-65, 54% women) who were assessed monthly for 2 years. RESULTS: Lifetime adversity did not show steeling effects, but instead appeared to exacerbate the impact of monthly adversity on psychological well-being, indicating cumulative disadvantage. On months where an adversity was reported, on average, individuals who reported more lifetime adversity showed stronger increases in depressive symptoms, anxiety, and negative affect and decreases in positive affect. There was limited evidence to suggest for steeling effects for life satisfaction. Reporting adversity in the personal, bereavement, social-environmental, and relationship domains showed the strongest associations with psychological well-being. DISCUSSION: Our discussion focuses on how lifetime adversity showed a cumulative disadvantage effect on the consequences of monthly adversity on psychological well-being. We also elaborate on future directions for research that include other conceptualizations of adversity and research to examine mechanisms underlying this relationship.

Neuroendocrine and autonomic stress systems activity in young adults raised by mothers with mental health and substance abuse problems: A prospective cohort study.

Among the well-known physiological consequences of early adverse environments is dysregulation of the hypothalamic-pituitary-adrenal (HPA) axis. A number of studies demonstrate that negative parenting and living with parents with a history of substance abuse and mental health problems may be associated with HPA axis dysregulation in children. In contrast, studies of more delayed effects in adult offspring, especially prospective, are still scarce. This study was a prospective longitudinal investigation of the association between maternal mental illnesses/substance abuse and maternal negative parenting/parental stress on one side and, on the other side, adult offspring outcomes 10 years later-specifically, we studied the activity of offspring's neuroendocrine (cortisol) and autonomic (heart rate) systems when exposed to a mild psychological stressor. Children of mothers with mental illnesses and/or substance abuse were exposed to more disadvantaged conditions (higher negative parenting and community violence). Despite this, maternal risk groups (having a mother with mental illnesses and/or substance abuse) were not associated with any of the indicators of stress systems activity. Regardless of the risk group, participants with dysregulated HPA axis activity experienced a higher level of negative parenting. Altogether, our study provides evidence that negative parenting may have long-lasting effects on stress-sensitive physiological mechanisms.

Risk and resilience among Asian American youth: Ramifications of discrimination and low authenticity in self-presentations.

This study examines adjustment patterns among a group neglected in developmental science-Asian American students in high-achieving schools. National reports have declared such schools to connote risk for elevated problems among teens. Asian American students are commonly referred to as model minorities, but little is known about adjustment issues within academically competitive settings, specifically. Guided by past research on culturally salient issues, multiple U.S. high schools were examined to (a) determine areas of relative strength versus weakness in adjustment of Asian Americans compared with Whites, and (b) more importantly, to illuminate salient within-group processes related to Asian Americans' well-being. Risk modifiers examined were perceptions of ethnic discrimination, parent perfectionism, internalized achievement pressure, authenticity in self-presentation, and closeness to school adults. Outcome variables included depression, anxiety, and isolation at school. Results demonstrated that Asian Americans fared better than Whites on anxiety and school isolation, but with low effect sizes. By contrast, they fared more poorly on almost all risk modifiers, with a large effect size on discrimination. Regression results showed that among Asian Americans the most consistent associations, across cohorts and outcomes, were for discrimination and authenticity. Findings underscore the need for greater recognition that discrimination could be inimical for students not typically thought of as vulnerable-Asian Americans in high-achieving schools; these issues are especially pressing in light of increased racism following coronavirus disease 2019 (COVID-19). Results also suggest that feelings of inauthenticity could be a marker of generalized vulnerability to internalizing symptoms. Implications for future theory and interventions are discussed. (PsycInfo Database Record (c) 2021 APA, all rights reserved).

Adverse childhood experiences among youth from high-achieving schools: Appraising vulnerability processes toward fostering resilience.

Among youth from high-achieving schools, adverse childhood experiences (ACEs) were examined in relation to (a) internalizing and externalizing symptoms in adolescence (n = 527), and (b) symptoms plus psychiatric diagnoses-based on multiple annual interviews-in adulthood (n = 316). Also examined were associations for a "Proxy ACEs" (P-ACEs) measure, containing items similar to those on standard ACEs measures without reference to abuse or neglect. Rates of ACEs were comparable with those in other studies; most commonly endorsed were perceived parental depression followed by aspects of emotional neglect. Groups exposed to zero, 1, 2, 3, and 4+ ACEs differed on symptoms in adulthood, with small to moderate effect sizes; in parallel comparisons of P-ACEs groups on Grade 12 symptoms, differences had large effect sizes. In relation to psychiatric diagnoses, comparisons with the zero ACEs group showed that groups with 1, 2, 3 ACEs, versus 4+ ACES, respectively, had twofold and over fivefold greater odds of having any lifetime diagnosis. The odds for internalizing diagnoses specifically were 2-6 times greater for individuals with 1, 2, and 3 ACEs, and 12 times greater for those reporting 4 ACEs. Remarkably, Grade 12 reports of 2, 3, and 4+ P-ACEs were linked to 2-3 times greater odds of a psychiatric disorder in adulthood, and 3-6 times greater odds for internalizing diagnoses specifically. In the future, assessments of ACEs and P-ACEs could facilitate early detection of problems among HAS students, informing interventions to mitigate vulnerability processes and promote resilience among these youth and their families. (PsycInfo Database Record (c) 2021 APA, all rights reserved).

Risk and resilience during COVID-19: A new study in the Zigler paradigm of developmental science.

When children are exposed to serious life adversities, Ed Zigler believed that developmental scientists must expediently strive to illuminate the most critical directions for beneficial interventions. In this paper, we present a new study on risk and resilience on adolescents during COVID-19, bookended - in introductory and concluding discussions - by descriptions of programmatic work anchored in lessons learned from Zigler. The new study was conducted during the first two months of the pandemic, using a mixed-methods approach with a sample of over 2,000 students across five high schools. Overall, rates of clinically significant symptoms were generally lower as compared to norms documented in 2019. Multivariate regressions showed that the most robust, unique associations with teens' distress were with feelings of stress around parents and support received from them. Open ended responses to three questions highlighted concerns about schoolwork and college, but equally, emphasized worries about families' well-being, and positive outreach from school adults. The findings have recurred across subsequent school assessments, and strongly resonate with contemporary perspectives on resilience in science and policy. If serious distress is to be averted among youth under high stress, interventions must attend not just to the children's mental health but that of salient caregiving adults at home and school. The article concludes with some specific recommendations for community-based initiatives to address mental health through continued uncertainties of the pandemic.

The Well-Being Index (WBI) for schools: A brief measure of adolescents' mental health.

Schools are increasingly concerned with the well-being of the whole child - likely, more so since the COVID-19 pandemic - and goals here were to document the psychometric properties of a brief new measure of adolescent mental health, the Well-Being Index (WBI). The measure assesses 4 symptom areas, 2 each of internalizing and externalizing symptoms-Depression, Anxiety, Rule-Breaking, and Substance Use-and an optional scale on Isolation at School. A total of 2,444 students from 2 high schools completed the WBI, the Youth Self-Report (YSR), and other related measures. Alpha coefficients showed acceptable internal consistency, with values for the 5 WBI subscales at .83, .84, .78, .79, and .74, respectively. Both exploratory and confirmatory factor analyses demonstrated consistent factorial validity. Correlations with corresponding YSR subscales indicated good convergent and discriminant validity. The WBI Substance Use and Isolation at School subscales, similarly, had high correlations with subscales from preexisting measures. Criterion-related validity was indicated in significant correlations between WBI subscales and conceptually related dimensions of close relationships. Also examined was the percentage of youth falling above clinical cutoffs on both the WBI and YSR, and findings demonstrated high concurrent validity. Collectively, results suggest the promise of the WBI as a brief, psychometrically sound measure to assess the adjustment of adolescents, along with perceptions of school climate that can be modified toward fostering their overall well-being. (PsycInfo Database Record (c) 2020 APA, all rights reserved).

Edward Zigler (1930-2019).

Presents an obituary for Edward Zeigler (1930-2019). Yale University's Sterling Professor Emeritus of Psychology Edward Zigler often encouraged his students and junior colleagues with the refrain, "You are doing God's work," but warned them that they would have to be ready to "Lose, lose, lose" in the process. This dual-pronged exhortation reflected Ed's value that no cause is greater than that of improving the lives of children and their families who are vulnerable because of life circumstances. For more than half a century, Ed was a tireless and devoted champion for children with intellectual disability, children born into poverty, children from minority backgrounds, adults with psychopathology, and many other marginalized groups. Ed's academic legacy is enshrined by his more than 800 scholarly articles, 43 books and monographs, the numerous social and educational programs that have positively impacted millions of children and families in the United States and around the world, and his generations of students who have contributed to the well-being of children and families in many different ways. This extensive and varied tapestry of accomplishments reflects Ed's world view that success in advocacy, public policy, and social programming is dependent on meticulous science, and that science is only meaningful when it enhances the lives of others. (PsycInfo Database Record (c) 2020 APA, all rights reserved).

Introduction to the special issue: Socialization of emotion and self-regulation: Understanding processes and application.

Over 20 years ago, Eisenberg, Cumberland, and Spinrad (1998; Eisenberg, Spinrad, & Cumberland, 1998) published a landmark article focusing on the socialization of children's emotion and self-regulation, including emotion regulation. In this special issue, our goal was to compile current evidence delineating the impact of emotion-related socialization behaviors (ERSBs) on children's emotion, self-regulation, and developmental outcomes. The work in this issue highlights the processes involved in predicting both parents' ERSBs as well as children's developmental outcomes. Researchers have moved beyond testing individual "pieces" of the socialization of emotion model and now use innovative and sophisticated methods for testing larger models, allowing for more causal interpretations. Special issue contributors focused on longitudinal studies including ERSBs, reviews of the literature extending the original model, and the effectiveness of interventions designed to improve the emotional lives of children and their families. We focus on some of the major themes of the special issue and conclude with recommendations for policies and programs to promote youths' effective emotion-related outcomes. (PsycINFO Database Record (c) 2020 APA, all rights reserved).

Parental aggravation may tell more about a child's mental/behavioral health than Adverse Childhood Experiences: Using the 2016 National Survey of Children's Health.

BACKGROUND: Adverse childhood experiences (ACEs) are associated with a number of health problems and early mortality. Developmental studies have also shown strong links between parents' contemporaneous negative feelings toward their children and the children's maladjustment. OBJECTIVES: The relative, unique contributions of ACEs and parents' feelings of aggravation were examined in predicting to the presence of children's internalizing and externalizing problems, perseverance and emotional regulation. Also tested was the potential moderating roles of personal support and external emotional resources for parents. PARTICIPANTS AND SETTING: Data from the 2016 National Survey of Children's Health were used. A random, nationally representative sample of 35,718 adult caregivers in the United States (US) with children ages 6-17 were included. METHODS: Hierarchical multiple regression analyses were performed to explore the patterns of results in predicting to children's maladjustment and adjustment, separately by child sex. RESULTS: Parental aggravation consistently had larger effects on children's maladjustment and adjustment than ACEs (1.47-1.82 timesamong males; 1.31-1.83 time among females, with one exception, i.e. internalizing problems). Personal support for parenting attenuated the relations of both ACEs and parental aggravation with children's outcomes. In the presence of external resources for parenting, children's maladjustments tended to be even more pronounced, suggesting that parents seek external resources when problem behaviors become significant in their children. CONCLUSION: For children at risk, future interventions should consider the value of refocusing attention from the occurrence ACEs per se, to critical, proximal indices - parents' negative feelings around parenting - that can have stronger links with children's maladjustment and that are more amenable to change.

High-achieving schools connote risks for adolescents: Problems documented, processes implicated, and directions for interventions.

Excessive pressures to excel, generally in affluent contexts, are now listed among the top 4 "high risk" factors for adolescents' mental health, along with exposure to poverty, trauma, and discrimination. Multiple studies of high-achieving school (HAS) cohorts have shown elevated rates of serious symptoms relative to norms, with corroborating evidence from other research using diverse designs. Grounded in theories on resilience and ecological influences in development, a conceptual model is presented here on major risk and protective processes implicated in unrelenting achievement pressures facing HAS youth. These include forces at the macrolevel, including economic and technological changes that have led to the "middle class squeeze," and proximal influences involving the family, peers, schools, and communities. Also considered are potential directions for future interventions, with precautions about some practices that are currently widespread in HAS contexts. In the years ahead, any meaningful reductions in the high distress of HAS youth will require collaborations among all stakeholders, with parents and educators targeting the specific areas that must be prioritized in their own communities. Leaders in higher education and social policy could also help in beginning to curtail this problem, which is truly becoming an epidemic among today's youth. (PsycInfo Database Record (c) 2020 APA, all rights reserved).

Toward fostering resilience on a large scale: Connecting communities of caregivers.

In interventions for at-risk children, Tom Dishion strongly exhorted programs that are short term, cost-effective, and delivered in families' own communities, just as resilience researchers underscore the need for programs that provide ongoing support for children's primary caregivers, and are implementable on a large scale. Presented here are preliminary results on a short-term intervention for mothers, the Authentic Connections Virtual Groups. A previous randomized trial of the in-person version of this program, conducted with mothers at high risk for stress and burnout, showed significant benefits. There had been zero dropouts across the 3-month program, and participants showed significant improvements on psychological indices as well as cortisol, even 3 months after the program ended. In the present study, virtual groups were conducted with five sets of women, all white-collar professionals with highly stressful, exacting careers, and most also primary caregivers of their children. Again, there were zero dropouts. Mean satisfaction ratings were 9.6 of 10, and the Net Promoter Score (promoters vs. detractors) fell in the "world class" range. To illuminate mechanisms of change, participants' responses to open-ended questions on the groups' value are presented verbatim. Recurrently mentioned were the development of new, authentic connections and invaluable ongoing support. These results, with the low costs and ease of women's attendance, attest to the value of expanding offerings such as these, toward benefiting even more highly stressed mothers themselves as well as the children for whose care they are responsible.

Examining the link between emotional childhood abuse and social relationships in midlife: The moderating role of the oxytocin receptor gene.

BACKGROUND: A single nucleotide polymorphism (SNP), rs53576, of the oxytocin receptor gene (OXTR) has been associated with fundamental aspects of social processes and behaviors. Compared to A carriers, GG individuals have enhanced social competencies and tend to elicit more positive responses from social partners. However, the G allele of the OXTR rs53576 has also been associated with greater social sensitivity. OBJECTIVE: The current study examined the unique influence of emotional childhood abuse on positive and negative aspects of different types of social relationships (e.g., family, spouse/partner, and friends) in midlife and whether genetic variations of OXTR rs53576 moderated these associations. PARTICIPANTS AND SETTING: A total of 614 participants in midlife (aged 40-65), recruited for a large-scale study of Phoenix metropolitan residents (AS U Live Project), answered self-report questionnaires and provided blood samples for DNA genotyping. METHODS: Hierarchical multiple regression analyses tested whether emotional childhood abuse predicted social support and strain for each relationship type and whether these potential linkages differed by OXTR genotype (GG versus AA/AG). RESULTS: Overall, individuals with a history of emotional childhood abuse had less supportive and more strained relationships in midlife. For supportive family relationships, the effect of emotional childhood abuse was moderated by OXTR rs53576 (p < .001). Under conditions of experiencing more emotional abuse in childhood, GG individuals had more supportive family relationships in midlife compared to A carriers. CONCLUSIONS: Overall, genetic variations of OXTR rs53576 may be an important candidate in understanding the development of social functioning within the context of emotional childhood abuse.

Invisible Household Labor and Ramifications for Adjustment: Mothers as Captains of Households.

We address the issue of invisible labor in the home by examining how the distribution of the mental and emotional labor inherent to managing the household between spouses may be linked with women's well-being, including their satisfaction with life, partner satisfaction, feelings of emptiness, and experiencing role overload. In a sample of 393 U.S. married/partnered mothers, mostly of upper-middle class backgrounds with dependent children at home, results showed that a majority of women reported that they alone assumed responsibility for household routines involving organizing schedules for the family and maintaining order in the home. Some aspects of responsibilities related to child adjustment were primarily handled by mothers, including being vigilant of children's emotions, whereas other aspects were shared with partners, including instilling values in the children. Responsibility was largely shared for household finances. Regression analyses showed that after controlling for dimensions of emotional and physical intimacy, feeling disproportionately responsible for household management, especially child adjustment, was associated with strains on mothers' personal well-being as well as lower satisfaction with the relationship. The implications of our work highlight the need to consider the burden of household management on mothers' well-being and speak to mothers' own needs for support and care as the primary manager of the household. In future research on division of labor, it will be useful to measure these critical but often neglected dimensions of who coordinates the household, given potential ramifications of this dimension for the quality of marriages and women's personal well-being.

Middle school peer reputation in high-achieving schools: Ramifications for maladjustment versus competence by age 18.

In an upper-middle class setting, we explored associations between students' peer reputation in Grades 6 and 7 with adjustment at Grade 12. With a sample of 209 students, a confirmatory factor analysis (CFA) of peer reputation dimensions supported a 4-factor model (i.e., popular, prosocial, aggressive, isolated). Structural equation models were used to examine prospective links between middle school peer reputation and diverse Grade 12 adjustment indices, including academic achievement (Scholastic Aptitude Test scores and grade point average), internalizing and externalizing symptoms, and use of cigarettes, alcohol, and marijuana. Prosocial reputation was connected to higher academic achievement levels and fewer externalizing symptoms. Both prosocial and isolated reputations were negatively associated with dimensions of substance use, whereas popularity was positively associated. Implications for future research and interventions are discussed.

Negative parenting modulates the association between mother's DNA methylation profiles and adult offspring depression.

This study aimed to examine whether the relationship between mothers' DNA methylation profiles and offspring's depression is modulated by negative parenting. The participants were 35 African-American mother-offspring dyads. Young adult offspring (19 females; age = 17-29.5 years) were assessed on depressive symptoms, and mothers (36-51 years) were assessed on negative parenting. Methyl-binding domain (MBD) sequencing was used to assay genome-wide DNA methylation in peripheral T lymphocytes. Controlling for the effect of offspring's DNA methylation, mothers' DNA methylation was positively associated with offspring's depression. Hypermethylation of a subset of the epigenetic markers was associated with increased negative parenting. Negative parenting was positively correlated with offspring's depression as well, suggesting that negative parenting may be a modulator between the mother's epigenome and offspring's depression. This study is one of the first investigations of the modulating role of parenting behavior in associations between the mother's epigenome and offspring's depression.