Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 1 de 1
Filter
Add more filters










Database
Language
Publication year range
1.
J Pharm Pharmacol ; 58(6): 859-66, 2006 Jun.
Article in English | MEDLINE | ID: mdl-16734988

ABSTRACT

We examined the effect of saikogenin D on arachidonic acid metabolism in C6 rat glioma cells to clarify its anti-inflammatory mechanism. Incubation of C6 cells with saikogenin D for 20 min resulted in the inhibition of prostaglandin E(2) production and the accumulation of an arachidonic acid metabolite that was found to be 11,12-dihydroxyeicosatrienoic acid, a metabolite of 11,12-epoxyeicosatrienoic acid. C6 cells expressed rat epoxygenase mRNAs, CYP1A1, CYP2B1 and CYP2J3, which converted arachidonic acid to epoxyeicosatrienoic acids. 11,12-Epoxyeicosatrienoic acid inhibited A23187-induced prostaglandin E(2) production and SKF-525A, an inhibitor of epoxygenase, attenuated the saikogenin D-induced inhibition of prostaglandin E(2) production in C6 cells. Furthermore, 11,12-epoxyeicosatrienoic acid and 11,12-dihydroxyeicosatrienoic acid, but not saikogenin D, inhibited the activity of cyclooxygenase in a cell-free condition. These data suggest that saikogenin D activates epoxygenases that rapidly convert arachidonic acid to epoxyeicosanoids and dihydroxyeicosatrienoic acids, and then the metabolites secondarily inhibit prostaglandin E(2) production.


Subject(s)
Anti-Inflammatory Agents/pharmacology , Cytochrome P-450 CYP1A1/physiology , Cytochrome P-450 CYP2B1/physiology , Cytochrome P-450 Enzyme System/physiology , Dinoprostone/biosynthesis , Oleanolic Acid/analogs & derivatives , Oxygenases/physiology , 8,11,14-Eicosatrienoic Acid/analogs & derivatives , 8,11,14-Eicosatrienoic Acid/pharmacology , Animals , Arachidonic Acid/metabolism , Cell Line, Tumor , Cytochrome P-450 CYP1A1/genetics , Cytochrome P-450 CYP2B1/genetics , Cytochrome P-450 Enzyme System/genetics , Enzyme Activation/drug effects , Oleanolic Acid/pharmacology , Oxygenases/genetics , Proadifen/pharmacology , Prostaglandin-Endoperoxide Synthases/metabolism , RNA, Messenger/analysis , Rats
SELECTION OF CITATIONS
SEARCH DETAIL
...