ABSTRACT
In this study, the complete mitochondrial genome of Pangasius sanitwongsei was firstly reported and analyzed. It had a double-stand DNA molecule with 16536 bp in length, consisting of 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes and one control region. The structural organization and gene order was similar to other bony fishes. The complete mitochondrial genome of P. sanitwongsei provided in this work would be helpful for further research on phylogenetics and conservation genetics of the Siluriformes and other orders.
ABSTRACT
Colors are important phenotypic traits for fitness under natural conditions in vertebrates. Previous studies have reported several functional genes and genetic variations of pigmentation, but the formation mechanisms of various skin coloration remained ambiguous in fish. Jinbian carp, a common carp variant, displays two colors (yellow and black) in the skin, thus, it is a good model for investigating the genetic basis of pigmentation. In the present study, using the Jinbian carp as model, isobaric tags for relative and absolute quantification (ITRAQ) proteomics analysis was performed for yellow and black skin, respectively. The results showed that 467 differentially expressed proteins (DEPs) were identified between the yellow skin and the black skin. Similar to mammals, the up-regulated DEPs in black skin included UV excision repair protein RAD23 homolog A (Rad23a), melanoregulin (mreg), 5,6-dihydroxyindole-2-carboxylic acid oxidase5 (tyrp1) and melanocyte protein PMEL (PMEL), which were mainly grouped into melanogenesis pathway. However, several up-regulated DEPs in yellow skin were mainly enriched in nucleotide metabolism, such as GTPase IMAP family member 5 (GIMAP5), AMP deaminase 1 (AMPD1), adenosylhomocysteinase b (ahcy-b), and pyruvate kinase (PKM). In summary, several candidate proteins and their enrichment pathways for color variation in Jinbian carp were identified, which may be responsible for the formation of different colorations.
ABSTRACT
In this study, the complete mitochondrial genome of Silurus cochinchinensis was reported to be 16,501 bp in length, consisting of 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes, and one control region. The structural organization and gene order were equivalent to other bony fishes. The complete genome of S. cochinchinensis would provide the basic dataset for further research on phylogenetics and conservation genetics of catfishes.
ABSTRACT
Jinbian carp (Cyprinus carpio) is an endemic species in China. The complete mitochondrial genome of Jinbian carp is determined to be 16,581 bp in length and includes 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes, and a control region. Its structural organization and gene order are equivalent to other common carp strains. The phylogenetic analyses will contribute to further insights of the taxonomy and phylogeny in Cyprinidae family.
ABSTRACT
The complete mitochondrial genome of Parachromis managuensis (Perciformes: Cichlidae) is sequenced in this study. The genome sequence was 16 526 bp in length, with the base composition of 28.1% A, 25.5% T, 15.4% G, 31.0% C. The gene order and genes are the same as found in other previously reported cichlid fish, including 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes and 1 D-loop region. Except for ND6 gene and eight tRNA genes, all other mitochondrial genes were encoded on the heavy strand. Parachromis managuensis was closely related to Amphilophus citrinellus and Petenia splendid, based on its complete mitochondrial genome sequences. This complete mitogenome data provide the basis for taxonomic and conservation research of Parachromis managuensis.
