ABSTRACT
CONCLUSION: Surgical resection of laryngeal squamous cell carcinoma (LSCC) could be associated with improved circulating myeloid dendritic cell (mDC) number and monocyte-derived dentritic cell (MoDC) function. Although adjunctive radiotherapy after surgery did not effect the normalization of mDC number, it may have an impact on MoDC function. OBJECTIVE: To investigate the effects of surgery and adjunctive radiotherapy on both circulating dendritic cells (DCs) and MoDCs of LSCC patients. SUBJECTS AND METHODS: Forty-six patients with LSCC and 15 age-matched healthy control subjects were enrolled in this study. Blood samples were taken before, during, and after conventional treatment from both patients who underwent surgery only (n=18) and those who had adjunctive radiotherapy after tumor removal (n=28). Three-color flow cytometry was used for determination of circulating DC subsets. Moreover, MoDCs were generated utilizing granulocyte macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4), then the phenotype of MoDCs was measured by flow cytometry and the ability to stimulate autologous T cells was tested in a mixed leukocyte reaction (MLR). RESULTS: The preoperative mDC counts, MoDC surface molecular expression and stimulatory capacity were impaired in patients in comparison with controls. The number of mDCs and the expression of CD80, CD83, and HLA-DR on MoDCs were significantly increased as compared with those pretreatment in patients who underwent surgery only and in those who had surgery followed by adjunctive radiotherapy. However, the recovery of CD86 expression and allostimulatory activity was only observed in patients who underwent surgery alone.
Subject(s)
Carcinoma, Squamous Cell/immunology , Carcinoma, Squamous Cell/radiotherapy , Carcinoma, Squamous Cell/surgery , Dendritic Cells/immunology , Laryngeal Neoplasms/immunology , Laryngeal Neoplasms/radiotherapy , Laryngeal Neoplasms/surgery , Monocytes/immunology , Adult , Aged , Antigens, CD/blood , B7-1 Antigen/blood , Carcinoma, Squamous Cell/pathology , Combined Modality Therapy , Female , Flow Cytometry , HLA-DR Antigens/blood , Humans , Immune Tolerance/immunology , Immunoglobulins/blood , Laryngeal Neoplasms/pathology , Laryngectomy , Lymphocyte Activation/immunology , Lymphocyte Culture Test, Mixed , Male , Membrane Glycoproteins/blood , Middle Aged , Neck Dissection , Neoplasm Staging , Radiotherapy, Adjuvant , Reference Values , T-Lymphocytes/immunology , CD83 AntigenABSTRACT
BACKGROUND: The high postoperative recurrence of hepatocellular carcinoma (HCC) is a problem that would benefit from the identification of indicators of recurrence and prognosis. In the past few years, research has shown that E-cadherin and CD34 can be used as indicators of the invasion of malignant tumors. In the present study, we investigated the expression of E-cadherin and CD34 in HCC patients. METHODS: Expression levels of E-cadherin and CD34 in 41 HCC samples were detected using two-step immunohistochemical methods and compared with clinical pathological parameters and survival rate. RESULTS: The positive rates of E-cadherin and CD34 expression in 41 HCC cases were 48.78% and 100%, respectively. Expression of E-cadherin was significantly lower in patients with larger tumors, a high risk invasion and Edmondson classification III or IV (p<0.05). There was a significant relationship between CD34 expression and age and tumor invasiveness (p<0.05). There was no significant relationship between expression of CD34 and E-cadherin by Spearman statistical analysis (p>0.05). The survival rate in patients with negative expression of E-cadherin was significantly lower. CONCLUSIONS: The expression of CD34 cannot be used singly as a prognostic indicator for HCC patients. The co-expression of E-cadherin and CD34 cannot be used as a prognostic indicator for HCC patients. Clin Chem Lab Med 2008;46:1122-6.
