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1.
G3 (Bethesda) ; 5(8): 1703-11, 2015 Jun 12.
Article in English | MEDLINE | ID: mdl-26070844

ABSTRACT

The propensity to capture and mobilize gene fragments by the highly abundant Helitron family of transposable elements likely impacts the evolution of genes in Zea mays. These elements provide a substrate for natural selection by giving birth to chimeric transcripts by intertwining exons of disparate genes. They also capture flanking exons by read-through transcription. Here, we describe the expression of selected Helitrons in different maize inbred lines. We recently reported that these Helitrons produce multiple isoforms of transcripts in inbred B73 via alternative splicing. Despite sharing high degrees of sequence similarity, the splicing profile of Helitrons differed among various maize inbred lines. The comparison of Helitron sequences identified unique polymorphisms in inbred B73, which potentially give rise to the alternatively spliced sites utilized by transcript isoforms. Some alterations in splicing, however, do not have obvious explanations. These observations not only add another level to the creation of transcript diversity by Helitrons among inbred lines but also provide novel insights into the cis-acting elements governing splice-site selection during pre-mRNA processing.


Subject(s)
DNA Transposable Elements/genetics , RNA Precursors/metabolism , Zea mays/genetics , Alternative Splicing , Base Sequence , Genome, Plant , Molecular Sequence Data , Plant Roots/metabolism , Plant Shoots/metabolism , RNA Precursors/genetics
2.
PLoS One ; 8(12): e84149, 2013.
Article in English | MEDLINE | ID: mdl-24376789

ABSTRACT

The transition of Drosophila third instar larvae from feeding, photo-phobic foragers to non-feeding, photo-neutral wanderers is a classic behavioral switch that precedes pupariation. The neuronal network responsible for this behavior has recently begun to be defined. Previous genetic analyses have identified signaling components for food and light sensory inputs and neuropeptide hormonal outputs as being critical for the forager to wanderer transition. Trio is a Rho-Guanine Nucleotide Exchange Factor integrated into a variety of signaling networks including those governing axon pathfinding in early development. Sequoia is a pan-neuronally expressed zinc-finger transcription factor that governs dendrite and axon outgrowth. Using pre-pupal lethality as an endpoint, we have screened for dominant second-site enhancers of a weakly lethal trio mutant background. In these screens, an allele of sequoia has been identified. While these mutants have no obvious disruption of embryonic central nervous system architecture and survive to third instar larvae similar to controls, they retain forager behavior and thus fail to pupariate at high frequency.


Subject(s)
Alleles , Behavior, Animal , DNA-Binding Proteins/genetics , Drosophila Proteins/genetics , Drosophila melanogaster/genetics , Guanine Nucleotide Exchange Factors/genetics , Mutation , Nerve Tissue Proteins/genetics , Phenotype , Phosphoproteins/genetics , Protein Serine-Threonine Kinases/genetics , Animals , Female , Larva/genetics , Male , Pupa/genetics
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