ABSTRACT
The mutagenic activity of pBR322 bacterial plasmid DNA and of pBR322ins and pBR322insN recombinant plasmid DNAs has been investigated in Blld-ii-FAF 28 line cultivated fibroblasts of Chinese hamster. The pBR322 bacterial plasmid DNA is shown to induce no resistant mutations to 6-mercaptopurine; the pBR322insN (the frameshift mutation in human insulin gene; this gene is supposed to have no expression in the cells because of frameshift mutation) induces neither such mutations as well. The pBR322ins recombinant plasmid carrying the native human insulin gene induces the gene mutations in this system. The influence of the insulin gene structure on the gene mutations induction is taken into account. The results obtained and the data of other authors illustrate a great interest of recombinant DNA molecules carrying transgenes in the field of mutation research.
Subject(s)
DNA, Bacterial/genetics , DNA, Recombinant/genetics , Insulin/genetics , Mutagenesis/genetics , Plasmids/genetics , Animals , Base Sequence , Cell Line , Cells, Cultured/drug effects , Cricetinae , Cricetulus , Drug Resistance/genetics , Fibroblasts/drug effects , Humans , Mercaptopurine/antagonists & inhibitors , Molecular Sequence Data , Transfection/geneticsABSTRACT
The mutagenic activity of the pUC19 bacterial plasmid DNA and the pAins recombinant plasmid DNA carrying human insulin gene has been investigated. Both pUC19 and pAins plasmid DNAs have been shown to induce the gene mutations in hprt locus of Chinese hamster cell line. The high level of the gene mutations (similar to the indices of the gene mutations induced by the chemical mutagens) has been in the focus of attention. The conclusion has been made concerning impossibility to use pAins plasmid DNA in the diabetes mellitus gene therapy. It is necessary to test the mutagenic properties of the DNA molecules produced for gene therapy of human inherited diseases.
