ABSTRACT
Male guinea pigs, intact and orally sensitized with chick ovalbumin (OVA), having initial bw 250-300 g, received glycine intraperitoneally or orally in a single dose of 100 or 200 mg/kg/day, during 3 days. The specific volume of cytochromes P450 and b5 in the microsomal fraction of the liver, activity of aminopyrine N-demethylase and aniline-p-hydroxylase, severity of food anaphylaxis to OVA and the animals' sensitivity to intravenous histamine LD50 were studied. The inducing effect of glycine on the cytochrome P450 system in the liver was more pronounced in the presensitized animals, that was attended by a decreased severity of food anaphylaxis and by a protective action in respect to histamine LD50. Possible mechanisms of glycine action are considered regarding its participation in metabolic processes.
Subject(s)
Anaphylaxis/prevention & control , Cytochrome P-450 Enzyme System/biosynthesis , Food Hypersensitivity/prevention & control , Glycine/therapeutic use , Liver/enzymology , Anaphylaxis/enzymology , Animals , Food Hypersensitivity/enzymology , Guinea Pigs , Liver/drug effects , MaleABSTRACT
Immunization of animals of different species (mice, rats, guinea pigs) with a corpuscular antigen, ram erythrocytes, was shown to be followed by inhibition of cytochrome-P-450-dependent monooxygenase system of the liver.
Subject(s)
Cytochrome P-450 Enzyme System/metabolism , Immunization , Microsomes, Liver/enzymology , Animals , Antibodies/analysis , Erythrocytes/immunology , Guinea Pigs , Male , Mice , Mice, Inbred C57BL , Mice, Inbred CBA , Rats , Rats, Inbred Strains , Rosette Formation , Time FactorsABSTRACT
The effect of a single intraperitoneal injection of perfluorodecaline (0.5 ml) and perfluorotributylamine on the cytochrome P-450-dependent monooxygenase system of male hybrid mouse (CBA X C57Bl) liver was studied. It was shown that perfluorodecaline is a potent inducer of the cytochrome P-450 system in the liver. The sharp increase of the cytochrome P-450 content and of the enzymatic activity was observed within 5 months. The biological effect of perfluorotributylamine on the monooxygenase system of the liver was much less pronounced. A slight inhibition of the cytochrome P-450 system was observed at short times after the injection followed by its slight stimulation after 2 weeks; 30 days thereafter all the parameters under study returned to control values.
Subject(s)
Cytochrome P-450 Enzyme System/metabolism , Fluorocarbons/pharmacology , Liver/enzymology , Animals , Cytochrome P-450 Enzyme Inhibitors , Enzyme Activation/drug effects , Male , Mice , Time FactorsABSTRACT
The cytochrome P-450-dependent monooxygenase system of the liver was studied in laboratory noninbred male rats selected according to the intensity of their initial alcohol motivation and the dynamics of these parameters was followed up during 10-day alcoholisation. It was shown that in the animals inclined to the development of alcoholism the activity of the monooxygenase system (cytochrome P-450, B5; enzymes: aminopyrine N-demethylase, aniline p-hydroxylase, NADPH-cytochrome c-reductase) is higher than in the animals noninclined to the development of this disease. 10-day alcohol consumption in the free-choice situation between water and 15% ethanol solution did not change the parameters investigated. The only exception was NADPH-cytochrome c-reductase: its activity grew in both the groups of the animals by 40-75%.
Subject(s)
Alcoholism/enzymology , Cytochrome P-450 Enzyme System/metabolism , Liver/enzymology , Animals , Disease Susceptibility , Male , Oxygenases/metabolism , Rats , Time FactorsABSTRACT
Intraperitoneal injection of hydrocortisone in single daily doses from 0.1 to 25.0 mg/kg, for 3 days was followed by reduced activity of liver microsomal monooxygenases and decreased content of cytochrome P-450 and b5. Hydrocortisone diminished the activity of the liver cytochrome P-450 system in vivo that significantly potentiated hexenal sleep. The hydrocortisone-inhibited activity of the liver chromosome P-450 system was followed by intensified food anaphylaxis in guinea-pigs.
Subject(s)
Anaphylaxis/immunology , Cytochrome P-450 Enzyme Inhibitors , Food Hypersensitivity/immunology , Hydrocortisone/pharmacology , Liver/drug effects , Animals , Dose-Response Relationship, Drug , Drug Synergism , Egg Proteins/immunology , Guinea Pigs , Hexobarbital/pharmacology , Liver/enzymology , Male , Sleep/drug effectsSubject(s)
Anaphylaxis/etiology , Cytochrome P-450 Enzyme Inhibitors , Liver/enzymology , Animals , Chickens , Female , Guinea Pigs , Male , Ovalbumin/adverse effectsABSTRACT
The cytotoxic index was studied in 38 patients with melanoma. The cytotoxic effect would not be observed in tumor progression or in the persistent absence (1.5 years) of the signs of metastasization and recurrences in case of the previously removed tumor. The positive effect of the cytotoxic test in some cases was gained in the presence only of the primary focus or after the latter and metastases being removed, and in the long-term absence of metastases and recurrences in the period to come. In other cases the positive effect was gained during a short period of time before the appearance of metastases or in progressing of the disease. To fully estimate the results of the study the cytotoxic test should be methodically associated with studying of the blocking effect of patient's serum. The blood groups A. B. O compatibility or incompatibility of patient's and donor tumor cells did not influence the results of the cytotoxic test.
