Modeling atomic force microscopy and shell mechanical properties estimation of coated microbubbles.

We present an extensive comparison with experimental data of our theoretical/numerical model for the static response of coated microbubbles (MBs) subject to compression from an atomic force microscope (afm). The mechanics of the MB's coating is described in the context of elastic thin shell theory. The encapsulated fluid is treated as compressible/incompressible pertaining to a gas/liquid, while the thinning of the liquid film between the MB and the afm cantilever is modeled via introduction of an interaction potential and the resulting disjoining pressure. As the external force increases, the experimental force-deformation (f-d) curves of MBs covered with polymer have an initial linear response (Reissner regime), followed by a non-linear curved downwards response (Pogorelov regime) where buckling takes place. On the other hand, the f-d curve for MBs covered with lipid monolayers initially follows the Reissner regime, but buckling is bypassed to a curved upwards regime where internal gas pressure dominates. The elastic properties, namely Young's modulus and shell thickness, for MB's covered with polymer can be estimated by combining the buckling point and the slope of the Reissner regime or the slopes of Reissner and Pogorelov regimes. Comparison of the present model with afm f-d curves for polymer shows satisfactory agreement. The area dilatation and bending moduli are shown to be the appropriate independent elastic parameters of MBs covered with phospholipid monolayers and are estimated by combination of the transition from Reissner to pressure dominated regime. Simulations and experiments in this case are in excellent agreement.

Antiparasitic and immunomodulatory effect of innovative treatments against Myxobolus sp. infection in Diplodus puntazzo.

The potential antiparasitic and immunomodulatory effect of three treatments against myxosporean parasites on the innate immune system of sharpsnout sea bream (Diplodus puntazzo) was investigated. Fish naturally infected with Myxobolus sp. (Bivalvulida/Platysporina), a histozoic parasite mainly affecting the renal interstitial tissue, were treated by oral administration of a combination of salinomycin with amprolium, Origanum essential oil or fumagillin in a small-scale field trial. Various leucocyte functions influenced by myxosporean infection were examined in order to determine treatment effects on leucocyte immunocompetence of treated fish. One month post treatment all drugs caused a significant decrease in prevalence and intensity of infection in comparison to untreated, infected fish. The effect was most prominent in salinomycin with amprolium treated fish, which 1-month post treatment contained either no cysts at all or a few spores free in melanomacrophage centres revealing almost total elimination of the parasite and the antiparasitic action of the treatment. There was no histopathological evidence of drug toxicity. Antiparasitic action was accompanied by a significant enhancement of phagocytic activity demonstrated by ingestion of large numbers of latex beads and the secretion of high levels of reactive nitrogen intermediates by phagocytes in vitro. Complete restoration of the diminished mitogenic responses and serum lysozyme secretion was also detected in salinomycin with amprolium-treated fish compared to untreated, infected fish. These data suggest that salilomycin with amprolium may be a promising treatment for myxosporean infections in intensively cultured warm-water fish, exhibiting action partially via the enhancement of host, innate immune functions and leading to parasite elimination.