Top-down and bottom-up microbiome engineering approaches to enable biomanufacturing from waste biomass.

Growing environmental concerns and the need to adopt a circular economy have highlighted the importance of waste valorization for resource recovery. Microbial consortia-enabled biotechnologies have made significant developments in the biomanufacturing of valuable resources from waste biomass that serve as suitable alternatives to petrochemical-derived products. These microbial consortia are designed following a top-down or bottom-up engineering approach. The top-down approach is a classical method that uses environmental variables to selectively steer an existing microbial consortium to achieve a target function. While high-throughput sequencing has enabled microbial community characterization, the major challenge is to disentangle complex microbial interactions and manipulate the structure and function accordingly. Microbial consortia design through a bottom-up approach uses prior knowledge of the metabolic pathway and possible interactions among consortium partners to design and engineer synthetic microbial consortia. This strategy offers some control over the composition and function of the consortium for targeted bioprocesses, but challenges remain in optimal assembly methods and long-term stability. In this review, we present the recent advancements, challenges, and opportunities for further improvement using top-down and bottom-up approaches for microbiome engineering. As the bottom-up approach is relatively a new concept for waste valorization, this review explores the assembly and design of synthetic microbial consortia, ecological engineering principles to optimize microbial consortia, and metabolic engineering approaches for efficient conversion. Integration of top-down and bottom-up approaches along with developments in metabolic modeling to predict and optimize consortia function are also highlighted.

Evaluation of the soil profile quality of subsided land in a coal mining area backfilled with river sediment based on monitoring wheat growth biomass with UAV systems.

Underground coal mining leads to land subsidence, and the situation is particularly serious in the Coal-Grain Complex in eastern China, causing the crop production to be reduced or to be taken out. Backfilling with Yellow River sediment is one of the effective methods to solve the land subsidence in this area, but a key issue is how to select the optimal soil reconstruction profile so that the crop yield after backfilling and reclamation is unaffected. The main purpose of this study is to verify the feasibility of selecting the optimal soil reconstruction profile by rapid monitoring of crop growth and judging soil quality with the aid of unmanned aerial vehicle systems (UAVs). A control treatment and 13 experimental treatments were established for the study area. The control treatment consisted of using 30 cm topsoil and 90 cm subsoil and the topsoil is a proxy for native (undisturbed) soil from the study sites. All other treatments consisted of using varying combinations of subsoil and sediment overlain by 30 cm of topsoil. The vegetation indices from the UAV multispectral images, and the plant height and vegetation coverage from the UAV RGB images were used for estimation of the winter wheat biomass in a random forest regression. The results showed that the random forest regression model yielded accurate estimation of the aboveground biomass. Furthermore, knowledge of plant height and vegetation coverage improved the accuracy of prediction such that crop growth was well characterized. The optimal soil profile consisted of 0.3 m topsoil + 0.2 m subsoil + 0.2 m sediment + 0.2 m subsoil + 0.3 m sediment. A fast and effective airborne monitoring method for soil quality was established, thus providing greatly improved monitoring efficiency.