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Arch Inst Pasteur Tunis ; 75(3-4): 219-26, 1998.
Article in French | MEDLINE | ID: mdl-14666749

ABSTRACT

Tunisian grapevine culture is affected by many viruses caused by some phytovirus belonging to nepovirus, closterovirus and trichovirus groups. The present work deal with the economically important viroses identified in tunisian grapevines. We present here the development methods to detect these viruses in propagating material. The important viruses biologically, biochemically, serologically and using molecular techniques, characterised are: GFLV, GLRaV3 and GVB. The genetic polymorphism analysis was also carried and tunisian isolates were compared to previously described ones in literature.


Subject(s)
Closterovirus/genetics , Nepovirus/genetics , Plant Diseases/virology , Plant Viruses/genetics , Virology/methods , Vitis/virology , Closterovirus/chemistry , Closterovirus/classification , Closterovirus/isolation & purification , DNA, Viral/analysis , DNA, Viral/genetics , Enzyme-Linked Immunosorbent Assay/methods , Molecular Sequence Data , Nepovirus/chemistry , Nepovirus/classification , Nepovirus/isolation & purification , Plant Viruses/chemistry , Plant Viruses/classification , Plant Viruses/isolation & purification , Polymorphism, Genetic/genetics , Reverse Transcriptase Polymerase Chain Reaction/methods , Tunisia
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