Human Bifidobacterium strains as adjunct cultures in Spanish sheep milk cheese.

Three bifidobacteria strains of human origin (Bifidobacterium pseudolongum INIA P2, Bifidobacterium breve INIA P734, and Bifidobacterium longum INIA P678) were used as potential probiotic adjunct cultures for the manufacture of pasteurized sheep milk cheese. Bifidobacteria were inoculated at 5 to 6 log cfu/mL in milk vats. Microbiological, physicochemical, rheological, color, and sensory characteristics were determined at 7, 28, and 60 d of ripening. Counts of B. pseudolongum INIA P2 remained above 6 log cfu/g during 60 d of ripening as well as after further simulated gastrointestinal digestion of cheeses. Bifidobacterium breve INIA P734 counts remained stable during 28 d and decreased by less than 1 log unit after simulated digestion. Bifidobacterium longum INIA P678 counts dropped sharply during cheese manufacture and ripening and were below detection level after simulated digestion. Addition of bifidobacteria strains did not affect starter viability, cheese pH, dry matter, water activity, or salt content but significantly increased overall proteolysis and the concentration of some free amino acids. Cheeses with bifidobacteria exhibited no significant differences in most sensory characteristics with respect to control cheese. According to our results, B. breve INIA P734 and B. pseudolongum INIA P2 are promising candidates as probiotic adjunct cultures in fresh and semi-hard sheep milk cheese.

Bifidobacterial strains shared by mother and child as source of probiotics.

Importance of bifidobacteria as part of the infant intestinal microbiota has been highlighted. Their acquisition is influenced by the mode of birth and the feed regime afterwards, with a special role of the maternal microbiota. The presence of the same shared bifidobacterial strains between breast milk and infant faeces in 14 mother-infant pairs was assessed by means of pulsed-field gel electrophoresis (PFGE) genotyping. Four shared strains of Bifidobacterium breve (2), Bifidobacterium longum subsp. infantis and B. longum subsp. longum were found in breast milk-infant faeces pairs. Two years later, a second survey yielded four shared strains of the species Bifidobacterium adolescentis, Bifidobacterium bifidum, B. longum subsp. longum and Bifidobacterium pseudocatenulatum. Moreover, a B. bifidum strain was found to be shared by the infant faeces of the first study and the mother faeces tested two years later, pointing out a long term persistence. Some of the selected bifidobacterial strains showed probiotic potential due to their survival to gastrointestinal conditions and their ability to form biofilms.

Melanoma glúteo gigante / Giant Melanoma on the Buttock

No disponible

Microscopic properties of xenon plasmas for density and temperature regimes of laboratory astrophysics experiments on radiative shocks.

This work is divided into two parts. In the first one, a study of radiative properties (such as monochromatic and the Rosseland and Planck mean opacities, monochromatic emissivities, and radiative power loss) and of the average ionization and charge state distribution of xenon plasmas in a range of plasma conditions of interest in laboratory astrophysics and extreme ultraviolet lithography is performed. We have made a particular emphasis in the analysis of the validity of the assumption of local thermodynamic equilibrium and the influence of the atomic description in the calculation of the radiative properties. Using the results obtained in this study, in the second part of the work we have analyzed a radiative shock that propagated in xenon generated in an experiment carried out at the Prague Asterix Laser System. In particular, we have addressed the effect of plasma self-absorption in the radiative precursor, the influence of the radiation emitted from the shocked shell and the plasma self-emission in the radiative precursor, the cooling time in the cooling layer, and the possibility of thermal instabilities in the postshock region.

Ranibizumab intravítreo en el tratamiento de membrana neovascular subretiniana en un caso de coroidopatía puntata interna / Intravitreal ranibizumab in the treatment of subretinal neovascularization in a case of punctate inner choroidopathy

CASO CLÍNICO: Se presenta el caso de una mujer de 28 años con disminución de agudeza visual en ojo izquierdo (OI) que presentaba una imagen sugestiva de membrana neovascular subretiniana en OI y lesiones coriorretinianas bilaterales compatibles con coroidopatía punctata interna (PIC) que fue tratada con ranibizumab intravítreo, obteniendo excelentes resultados. DISCUSIÓN: Debe realizarse el diagnóstico diferencial de la PIC con el resto de «síndromes de puntos blancos» y con el síndrome de presunta histoplasmosis ocular (SPHO). Los fármacos anti-VEGF pueden ser una buena alternativa como tratamiento de este tipo de enfermedades cuando desarrollan una membrana neovascular subretiniana

CASE REPORT: We report the case of a 28-year old woman suffering loss of visual acuity in her left eye, who presented an image suggestive of a subretinal neovascular membrane in her left eye, and bilateral retinal lesions compatible with punctate inner choroidopathy (PIC). She was treated with intravitreal ranibizumab obtaining excellent results. DISCUSSION: The differential diagnosis must be made between PIC and the rest of "white dot syndromes" and the presumed ocular histoplasmosis syndrome (POHS). Antiangiogenic drugs may be a good alternative for the treatment of such diseases when they develop a subretinal neovascular membrane

[Intravitreal ranibizumab in the treatment of subretinal neovascularization in a case of punctate inner choroidopathy]. / Ranibizumab intravítreo en el tratamiento de membrana neovascular subretiniana en un caso de coroidopatía puntata interna.

CASE REPORT: We report the case of a 28-year old woman suffering loss of visual acuity in her left eye, who presented an image suggestive of a subretinal neovascular membrane in her left eye, and bilateral retinal lesions compatible with punctate inner choroidopathy (PIC). She was treated with intravitreal ranibizumab obtaining excellent results. DISCUSSION: The differential diagnosis must be made between PIC and the rest of "white dot syndromes" and the presumed ocular histoplasmosis syndrome (POHS). Antiangiogenic drugs may be a good alternative for the treatment of such diseases when they develop a subretinal neovascular membrane.

Modeling of population kinetics of plasmas that are not in local thermodynamic equilibrium, using a versatile collisional-radiative model based on analytical rates.

We discuss the modeling of population kinetics of nonequilibrium steady-state plasmas using a collisional-radiative model and code based on analytical rates (ABAKO). ABAKO can be applied to low-to-high Z ions for a wide range of laboratory plasma conditions: coronal, local thermodynamic equilibrium or nonlocal thermodynamic equilibrium, and optically thin or thick plasmas. ABAKO combines a set of analytical approximations to atomic rates, which yield substantial savings in computer running time, still comparing well with more elaborate codes and experimental data. A simple approximation to calculate the electron capture cross section in terms of the collisional excitation cross section has been adapted to work in a detailed-configuration-accounting approach, thus allowing autoionizing states to be explicitly included in the kinetics in a fast and efficient way. Radiation transport effects in the atomic kinetics due to line trapping in the plasma are taken into account via geometry-dependent escape factors. Since the kinetics problem often involves very large sparse matrices, an iterative method is used to perform the matrix inversion. In order to illustrate the capabilities of the model, we present a number of results which show that the ABAKO compares well with customized models and simulations of ion population distribution. The utility of ABAKO for plasma spectroscopic applications is also outlined.

[Eyelid contact allergic eczema caused by topical ketorolac tromethamine 0.5%]. / Eccema alérgico de contacto palpebral por ketorolaco trometamol 0,5% tópico.

CLINICAL CASE: A 65-year-old man had been treated with eye-drops containing NSAIDs (Ketorolac). He developed conjunctival injection, edematous swelling of the eyelids and periorbital dermatitis due to a contact allergy. Allergy to NSAIDs is uncommon. DISCUSSION: This is an unusual case because topical application of ketorolac is safe in the vast majority of ophthalmologic patients. However adverse events associated with Ketorolac are similar to that of other NSAIDs.

Eccema alérgico de contacto palpebral por ketorolaco trometamol 0.5% tópico / Eyelid contact allergic eczema caused by topical ketorolac tromethamine 0.5%

Caso clínico: Hombre de 65 años que había sidotratado con AINE tópico (Ketorolaco). El pacientepresentó inyección conjuntival, edema de párpados,y dermatitis periorbitaria por eccema alérgico decontacto secundario a AINE, un hecho raro en estetipo de tratamiento.Discusión: Este es un caso raro, ya que la aplicaciónde Ketorolaco tópico es seguro en la mayoríade los pacientes oftalmológicos. Además, las reaccionesadversas asociadas con el Ketorolaco sonsimilares a otros AINES

Clinical case: A 65-year-old man had been treated with eye-drops containing NSAIDs (Ketorolac). He developed conjunctival injection, edematous swelling of the eyelids and periorbital dermatitis due to a contact allergy. Allergy to NSAIDs is uncommon. Discussion: This is an unusual case because topical application of ketorolac is safe in the vast majority of ophthalmologic patients. However adverse events associated with Ketorolac are similar to that of other NSAIDs

Hiperbilirrubinemia neonatal. Peculiar debut del déficit de alfa-1-antitripsina / Neonatal hyperbilirrubinemia: a peculiar presentation of alpha-1-antitrypsin deficiency

La deficiencia de alfa-1-antitripsina (AlTT) es una enfermedad hereditaria que predispone a afección hepática en la infancia y a enfisema pulmonar en la edad adulta. Constituye la causa genética más común en el niño y de enfisema en el adulto; también es la que motiva más trasplantes hepáticos en niños y adolescentes. En nuestro medio presenta una prevalencia de 1 / 1.600-4.000 y una incidencia de 1 / 200 nacidos vivos. La A1TT es una glucoproteína inhibidora de proteasas séricas, codificada en el cromosoma 14 y que se hereda de forma autosómica recesiva. El fenotipo normal es el MM, y las mutaciones más frecuentes son las que asocian S y Z. Las manifestaciones clinicas más frecuentes en neonatos son la hiperbilirrubinemia conjugada, la colestasis y la hepatomegalia. Presentamos el caso de un recién nacido del sexo masculino ingresado por prematuridad e hiperbilirrubinemia directa, lo que motiva diferentes supuestos etiopatogénicos. Realizadas las pruebas sistemáticas y el estudio hepático, se determinó descenso de AlTT, y se decidió efectuar un estudio genético, en el que presentó un genotipo SS para el alelo de AlTT. La madre era SS y el padre MS, y ambos eran asintomáticos. En controles posteriores, se confirmó descenso en la cifra de AlTT, asociado a elevaciones puntuales de ésta como reactante de fase aguda, en relación con diversos procesos agudos. El interés de este caso radica en que, compartiendo la alteración genética con familiares de primer grado y, al ser éstos aparentemente asintomáticos, nuestro paciente debutó en el periodo neonatal inmediato; este hecho es peculiar en la bibliografía médica (AU)

Influencia de la etnia gitana en el ingreso hospitalario de lactantes y su patología prevalente / INfluence of gypsy ethnicity on hospital admission and diagnostic group among infants

Antecedentes: Se cree que algunos grupos étnicos enferman menos, sin que exista en nuestro medio objetivación científica, lo que justifica su estudio. Objetivo: Analizar la frecuencia de ingreso hospitalario y el tipo de enfermedad de lactantes según su etnia. Material y métodos: En el año 2000 se registraron 496 ingresos de niños entre 1 y 24 meses de edad. La variable grupo étnico clasificó 403 casos como etnia no gitana (81,25%) y 93 como etnia gitana (18,75%). A partir de la demografía de referencia se observaron las variables etnia y grupo de diagnóstico principal. Se utilizó análisis estadístico descriptivo de las diferentes variables, considerando diferencias significativas cuando p < 0,05. Resultados: El grupo etnia gitana representa el 6,51% de la población lactante, mientras que acapara el 18,75% de los ingresos hospitalarios (p < 0,05). El grupo étnico gitano registró 54,8% de reingresos frente al 20,80% del no gitano (p < 0,001). El grupo étnico gitano presentó mayor prevalencia de procesos respiratorios (35,48%) que el grupo etnia no gitana (22,58%) (p < 0,05). Sin embargo, fue mayor la proporción de patología digestiva en la etnia no gitana, 34,73% frente a 19,35% (p < 0,05). Los restantes grupos diagnósticos no presentaron diferencias significativas. Conclusión: La diferencia registrada entre la distribución demográfica y frecuencia de ingreso va en contra de la creencia de la mayor resistencia a enfermar del grupo étnico gitano. Las diferencias pueden deberse a las condiciones socioeconómicas, higiénicas, sanitarias y educacionales entre ambos grupos. Hecho significativo en los procesos de tipo respiratorio, probablemente por mayor hacinamiento. El estilo de vida de nuestra población asistida de etnia, no confiere mayor resistencia a la enfermedad (AU)

[Influence of gypsy ethnicity on hospital admission and diagnostic group among infants]. / Influencia de la etnia gitana en el ingreso hospitalario de lactantes y su patología prevalente.

BACKGROUND: Some ethnic groups are thought to have greater resistance to disease. However, scientific studies of this phenomenon are lacking in our environment. OBJECTIVE: To study hospitalization rates and type of disease among infants according to ethnic group. MATERIAL AND METHODS: In 2000, 496 infants aged between 1 and 24 months were admitted to our hospital. Using ethnic group as a variable, 403 infants (81.25 %) were classified as non-gypsy and 93 (18.75 %) as gypsy. Demographic data were used to compare the variables of ethnicity and the main diagnostic group. Descriptive statistical analysis of the variables was performed. Differences were considered significant when p < 0.05. RESULTS: The gypsy group represented 6.51 % of the infant population but accounted for 18.75 % of infant admissions (p < 0.05). The gypsy group accounted for 54.83 % of readmissions compared with 20.80 % in the non-gypsy group (p < 0.001). Respiratory disease was more prevalent in the gypsy group (35.48 %) than in the non-gypsy group (22.58 %) (p < 0.05). However, digestive problems were more frequent in the non-gypsy group (34.73 %) than in the gypsy group (19.35 %) (p < 0.05). No statistically significant differences were found in other diagnostic groups. CONCLUSION: The differences observed between demographic distribution and admission rate contradict the belief that members of the gypsy ethnic group show greater disease resistance. The differences in admission rate and diagnostic group may be due to socioeconomic, hygienic, health and educational differences between both groups. Differences in the prevalence of respiratory disease were probably due to overcrowding. Gypsy lifestyle in our population does not confer greater disease resistance.

[Ureteral endometriosis]. / Endometriosis ureteral.

OBJECTIVE: To report a case of ureteral endometriosis, an uncommon disease in the urological practice that is diagnosed late and may cause irreversible damage to the upper urinary tract. METHODS/RESULTS: A case of unilateral ureteral endometriosis is presented. After attempting hormonal therapy and endourological management, the patient eventually underwent aggressive surgical treatment. CONCLUSIONS: The diagnosis of ureteral endometriosis should be considered in women presenting with noncalculous renal obstruction, particularly premenopausal women of low parity or those who have had previous pelvic surgery. Only a high index of suspicion and the radiological supports may help to reduce the number of alarming nephrectomies associated to this condition.

[Histiocytoid hemangioma of the epididymis. A very unusual entity]. / Hemangioma histiocitoide de epidídimo. Una entidad de gran rareza.

The presence of vascular origin tumor in the epididymis and testicle, are today considered of low frequency. We report a case of histiocytoid (epithelioid) hemangioma of the epididymis. Its differential diagnosis with the adenomatoid tumor, is discussed.

Hemangioma histiocitoide de epididimo. Una entidad de gran rareza / Histiocytoid hemangioma of the epididymis. A very strange entity

La presencia de tumoraciones de origen vascular en el epidídimo y testículo, se consideran hoy en día de baja frecuencia. Presentamos un caso de hemangioma histiocitoide (epitelioide) de epidídimo. Se discute su diagnóstico diferencial con el tumor adenomatoide (AU)

[Mucinous adenocarcinoma. Unusual prostatic tumor]. / Adenocarcinoma mucinoso. Una tumoración prostática poco frecuente.

We report a case of mucinous adenocarcinoma of the prostate. A 80 years-old patient was diagnosed with a transrectal multiple biopsy. We comment on the special histological patterns of the tumor as well as the established criterion for an appropriate diagnosis.

[Cytomegalovirus retinitis in a pediatric AIDS patient]. / Retinitis por citomegalovirus en una paciente pediátrica diagnosticada de SIDA.

INTRODUCTION: Cytomegalovirus retinitis (CMV) is the most frequently found opportunistic eye infection in adults with AIDS, with mean incidence of 20%-50%. However, only 5% of children with AIDS have this infection. CLINICAL CASE: We present the case of a six year old girl with stage C3 AIDS diagnosed at the age of 20 months, who developed unilateral diffuse retinitis due to CMV. The infection involved the posterior pole of the right eye, with retinal atrophy along the temporal vascular arcodes, and an active advance front in the temporal macula. The optic nerve was not found to be involved although the peripheral areas of the retina were involved leading to rhegmatogenous detachment of the superotemporal retina. In view of the systemic deterioration of the patient, no specific anti-CMV treatment was given. The patient died of respiratory insufficiency a few weeks later. CONCLUSIONS: CMV retinitis in paediatric AIDS patients is usually associated with more severe illness and a poorer general health than the adult population. In view of the absence of symptoms in these patients, periodic ophthalmoscopic examinations should be done in those who have severe immunological deterioration.

[Malignant fibrohistiocytoma of the renal capsule. Report of a case and review of the literature]. / Fibrohistiocitoma maligno de cápsula renal. Aportación de un caso y revisión de la literatura.

Presentation of one case of malignant fibrohistiocytoma derived from the renal capsule. While conducting a bibliographic review the low incidence of this tumoration of renal origin was noted. The difficulties in differentiating this entity from a secondary renal invasion by a malignant fibrohistiocytoma derived from other retroperitoneal structures is commented. Brief review of current diagnosis and therapeutical management for this tumoral entity.