In vitro comparison of water displacement method and 3 Tesla MRI for MR-volumetry of the olfactory bulb: which sequence is appropriate?

RATIONALE AND OBJECTIVES: Magnetic resonance imaging olfactory bulb (OB) volumetry (OBV) is already used as a complementary prognostic tool to assess olfactory disorders. However, a reference standard in imaging for OBV has not been established. The aim of this in vitro study was to compare volumetric results of different magnetic resonance sequences for OBV at 3 T to genuine OB volumes measured by water displacement. MATERIALS AND METHODS: The volumes of 15 human cadaveric OBs were measured using the water displacement method in this institutional review board-approved prospective study. The magnetic resonance imaging protocol at 3 T included constructive interference in steady state (CISS), T2-weighted (T2w) three-dimensional (3D) sampling perfection with application-optimized contrasts using different flip-angle evolutions (SPACE), T2w two-dimensional (2D) turbo spin-echo (TSE), and T1-weighted (T1w) 3D fast low-angle shot (FLASH) sequences. Two blinded observers independently performed two OB volumetric assessments per bulbus and sequence. Intraobserver and interobserver reliabilities were assessed by intraclass correlation coefficients. Bland-Altman plots were analyzed to evaluate systematic biases and concordance correlation coefficients to assess reproducibility. RESULTS: For both observers, intraclass correlation coefficient analysis yielded almost perfect results for intraobserver reliability (CISS, 0.94-0.98; T2w 3D SPACE, 0.93-0.98; T2w 2D TSE, 0.98-0.98; T1w 3D FLASH, 0.95-0.99). Interobserver reliability showed almost perfect agreement for all sequences (CISS, 0.98; T2w 3D SPACE, 0.89; T2w 2D TSE, 0.93; T1w 3D FLASH, 0.97). The CISS sequence yielded the highest mean concordance correlation coefficient (0.95) and the highest combination of precision (0.97) and accuracy (0.98) values. In comparison with the water displacement method, Bland-Altman analyses revealed the lowest systematic bias (-0.5%) for the CISS sequence, followed by T1w 3D FLASH (-1.3%), T2w 3D SPACE (-7.5%), and T2w 2D TSE (-10.9%) sequences. CONCLUSIONS: Compared to the water displacement method, the CISS sequence is suited best to validly and reliably measure OB volumes because of its highest values for accuracy and precision and lowest systematic bias.

Reproducibility and repeatability of volumetric measurements for olfactory bulb volumetry: which method is appropriate? An update using 3 Tesla MRI.

RATIONALE AND OBJECTIVES: The aim of this study was to compare different sequences for olfactory bulb volumetry using 3-T magnetic resonance imaging, evaluating reproducibility, repeatability, and systematic biases. MATERIALS AND METHODS: Twenty-two volunteers underwent 3-T magnetic resonance imaging of the frontal skull base in this prospective study. Imaging included constructive interference in steady state (CISS), T2-weighted (T2w) three-dimensional (3D) sampling perfection with application-optimized contrasts using different flip-angle evolutions, and T2w two-dimensional (2D) turbo spin-echo sequences. Two observers independently performed two olfactory bulb volumetric studies per bulb and sequence. Intraobserver and interobserver reliability was assessed using intraclass correlation coefficients. For the evaluation of reproducibility, concordance correlation coefficients were determined, and for repeatability and systematic biases, Bland-Altman plots were analyzed. RESULTS: Intraclass correlation coefficient analysis of the specialized observer yielded almost perfect results for intraobserver reliability (0.94, 0.85, and 0.93 for the CISS, T2w 3D, and T2w 2D sequences, respectively). For the less experienced observer, the results were 0.86 0.78, and 0.74 for the CISS, T2w 3D, and T2w 2D sequences, respectively. Interobserver reliability showed almost perfect agreement for all sequences (0.92, 0.86, and 0.86, respectively). The CISS sequence yielded the highest concordance correlation coefficient (0.84), precision (0.85), and accuracy (0.99). Bland-Altman plot analyses revealed the lowest repeatability coefficients for the T2w 2D sequence. Volumetric measurements of T2w 2D imaging showed systematically lower volumetric results compared to the CISS sequence (-22.7%) and the T2w 3D sequence (-8.3%). CONCLUSIONS: Comparison of three imaging sequences for olfactory bulb volumetry yielded the best values for the CISS sequence in terms of intraobserver and interobserver reliability, reproducibility, accuracy, and precision. Given that even less experienced observers achieve almost perfect results, the CISS sequence is recommended for olfactory bulb volumetry.

Visual grading characteristics (VGC) analysis of diagnostic image quality for high resolution 3 Tesla MRI volumetry of the olfactory bulb.

RATIONALE AND OBJECTIVES: The aim of this study was to evaluate the suitability of 3-T magnetic resonance imaging (MRI) for olfactory bulb volumetry, comparing image quality obtained using different sequences on the basis of physical characteristics in combination with observer performance. MATERIALS AND METHODS: Twenty-two healthy volunteers (11 men, 11 women; mean age, 25 years) underwent 3-T MRI of the frontal skull base in this prospective study. Imaging was performed using a constructive interference in steady state (CISS) three-dimensional Fourier transformation sequence, a three-dimensional T2-weighted (3D-T2w) sequence, and a two-dimensional T2-weighted (2D-T2w) sequence. The relative performance of sequences was assessed using visual grading characteristic analysis. Interobserver agreement was assessed using κ statistics. For evaluation of physical image quality characteristics, signal-to-noise ratio (SNR) and contrast-to-noise ratio (CNR) were calculated and compared using Wilcoxon's test. SNR and CNR measurements were correlated with visual grading results. RESULTS: Visual grading characteristic analysis showed significantly better image quality ratings for the CISS sequence compared to the 3D-T2w and 2D-T2w sequence, and the 2D-T2w sequence performed significantly better compared to the 3D-T2w sequence (P < .001). Interobserver agreement was substantial (κ = 0.66-0.73). Wilcoxon's test revealed significantly higher SNR and CNR values for the 2D-T2w sequence compared to the 3D-T2w and CISS sequences, and SNR and CNR values for the 3D-T2w sequence were significantly higher compared to those for the CISS sequence (P < .001 for each). Significant correlation between SNR and CNR and visual grading was found for the 3D-T2w sequence (SNR: ρ = 0.510, P = .015; CNR: ρ = 0.546, P = .009). CONCLUSIONS: High-resolution 3-T MRI resulted in excellent values for SNR and CNR, suggesting the appropriateness of the sequences for olfactory bulb MRI volumetry. Visual grading characteristic analysis revealed the CISS sequence to be the most suitable for olfactory bulb volumetry.