Search | VHL Regional Portal

A new multiplex PCR for easy screening of methicillin-resistant Staphylococcus aureus SCCmec types I-V.

Boye, K; Bartels, M D; Andersen, I S; Møller, J A; Westh, H.

Clin Microbiol Infect ; 13(7): 725-7, 2007 Jul.

Article in English | MEDLINE | ID: mdl-17403127

ABSTRACT

A multiplex PCR with four primer-pairs was designed to identify the five main known SCCmec types. A clear and easily discriminated band pattern was obtained for all five types. The SCCmec type was identified for 98% of 312 clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA). SCCmec type IV was by far the most common SCCmec type among both hospital- and community-acquired MRSA isolates in Denmark.

Subject(s)

Bacterial Typing Techniques , Methicillin Resistance/genetics , Polymerase Chain Reaction/methods , Staphylococcal Infections/diagnosis , Staphylococcus aureus/classification , Staphylococcus aureus/drug effects , Bacterial Proteins/genetics , Chromosomes, Bacterial/genetics , Community-Acquired Infections/diagnosis , Community-Acquired Infections/epidemiology , Community-Acquired Infections/microbiology , Cross Infection/diagnosis , Cross Infection/epidemiology , Cross Infection/microbiology , DNA Primers , Denmark/epidemiology , Humans , Mass Screening/methods , Penicillin-Binding Proteins , Staphylococcal Infections/epidemiology , Staphylococcal Infections/microbiology , Staphylococcus aureus/genetics , Staphylococcus aureus/isolation & purification

ABSTRACT

Subject(s)

SEND TO:

SELECTION OF CITATIONS

SEARCH DETAIL