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Int J Pept Protein Res ; 9(1): 78-9, 1977 Jan.
Article in English | MEDLINE | ID: mdl-838533

ABSTRACT

A simple method is described for the purification of peptide-M and MCD-peptide from bee venom. The steps are Sephadex G-50 gel filtration of pH 4.5 (Steps 1 and 2) and chromatography on silica gel (Steps 3 and 4). The peptides are homo geneous with respect to thin-layer chromatography, electrophoresis at pH 2.0, and amino acid analysis. Peptide-M contains 24 amino acid residues and has a calculated molecular weight of 2750.


Subject(s)
Bees , Peptides/isolation & purification , Venoms/analysis , Amino Acids/analysis , Animals , Chromatography, Gel , Chromatography, Thin Layer , Electrophoresis, Cellulose Acetate , Methods
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