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1.
Br J Cancer ; 89(4): 687-90, 2003 Aug 18.
Article in English | MEDLINE | ID: mdl-12915879

ABSTRACT

A recent report suggests that the KLF6 gene encoding the Krüppel-like factor 6 protein is a frequently mutated, putative tumour suppressor gene in prostate cancer. The aims of the present study were to confirm these initial findings by determining the frequency of exon2 KLF6 mutations in a cohort of European prostate cancer patients, and to investigate whether there was evidence for mutational inactivation of both the KLF6 and TP53 tumour suppressor loci in some tumours. We examined 32 primary prostate tumours and three prostate tumour cell lines for mutations by PCR amplification and direct dideoxy sequencing (KLF6), and by oligonucleotide microarray (p53GeneChip) analysis and dideoxy sequencing (TP53). Whereas TP53 mutations typical of prostate cancer were found at a frequency consistent with the literature, no KLF6 mutations were found in any of the tumour samples nor in the three prostate cancer cell lines.


Subject(s)
Genes, p53 , Point Mutation , Prostatic Neoplasms/genetics , Proto-Oncogene Proteins , Trans-Activators/genetics , Aged , Aged, 80 and over , DNA Mutational Analysis , DNA, Neoplasm/analysis , Exons , Humans , Kruppel-Like Factor 6 , Kruppel-Like Transcription Factors , Male , Middle Aged , Neoplasm Staging , Prostatic Neoplasms/pathology , Reverse Transcriptase Polymerase Chain Reaction , Tumor Cells, Cultured , Zinc Fingers/genetics
2.
Carcinogenesis ; 18(3): 511-6, 1997 Mar.
Article in English | MEDLINE | ID: mdl-9067550

ABSTRACT

The p53 gene was examined for point mutations in archived, alpha-radiation-associated lung and liver cancers. Lung tumors of 50 uranium miners in Germany were screened by restriction fragment length analysis for the putative hotspot mutation at codon 249 (Arg-->Met) previously detected in a significant fraction of miners from the Colorado Plateau, USA. This mutation has been proposed as a marker of radon exposure. None of the tumors we examined harbored the hotspot mutation. Five of the 50 tumors, however, did indeed harbor exon 7 mutations, as determined by subsequent mutation analysis of exon 7. These mutations were dispersed among various codons and may be attributable to heavy tobacco smoking in this cohort. In support of this interpretation, we found no mutations in exons 5-8 of the p53 gene in 13 iatrogenic liver cancers induced by injection of Thorotrast, an alpha-emitting radiocontrast agent. We propose that if the p53 tumor suppressor gene is a target for the carcinogenic action of alpha-particle radiation, loss of suppressor function may occur preferentially by mechanisms such as intrachromosomal deletions, rather than by base substitution mutations.


Subject(s)
Alpha Particles/adverse effects , DNA, Neoplasm/genetics , Genes, p53/radiation effects , Liver Neoplasms/genetics , Lung Neoplasms/genetics , Mining , Neoplasms, Radiation-Induced/genetics , Occupational Diseases/genetics , Point Mutation , Thorium Dioxide/adverse effects , Aged , Aged, 80 and over , Codon/genetics , Contrast Media/adverse effects , DNA Mutational Analysis , Exons/genetics , Germany/epidemiology , Humans , Liver Neoplasms/epidemiology , Liver Neoplasms/etiology , Lung Neoplasms/epidemiology , Lung Neoplasms/etiology , Male , Middle Aged , Neoplasms, Radiation-Induced/epidemiology , Neoplasms, Radiation-Induced/etiology , Occupational Diseases/epidemiology , Occupational Diseases/etiology , Polymorphism, Restriction Fragment Length , Radon/adverse effects , Smoking/adverse effects , Smoking/epidemiology , Smoking/genetics , Uranium/adverse effects
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