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1.
Urolithiasis ; 51(1): 120, 2023 Oct 06.
Article in English | MEDLINE | ID: mdl-37801124

ABSTRACT

This study is aimed to determine whether postoperative low dose computed tomography (LDCT) imaging is necessary after percutaneous nephrolithotomy (PCNL), or the surgeon's intraoperative assessment of residual fragments (RF) is sufficient and avoidance of postoperative imaging with reduction of radiation exposure can be achieved. Data of all 610 patients who underwent PCNL in prone position in our institution from February 2009 to September 2020 was collected. Parameters such as age, gender, BMI, ASA-Classification, stone related parameters and the surgeon's assessment of stone-free status were analyzed. The LDCT performed postoperatively was compared to the intraoperative assessment of the surgeon regarding RF. The mean age of patients was 52.82 years; the mean BMI was 28.18 kg/m2. In 418 cases, the surgeon made a clear statement about the presence of RF and postoperative LDCT was carried out. The discrepancy between the two methods (surgeon´s assessment vs. LDCT) was significant at p < 0.0001. The sensitivity, specificity, positive and negative predictive value of the surgeon when assessing RF were 24.05%, 99.45%, 98.28% and 50%. Stone free rate (SFR) after primary PCNL was 45.57%. The overall SFR at discharge was 96.23%. Although the surgeon´s assessment of RF was reliable, postoperative LDCT imaging should still be performed if endoscopic stone clearance is suspected due to the high false negative rate and the low negative predictive value. The optimal timing of postoperative imaging following PCNL remains unclear.


Subject(s)
Kidney Calculi , Nephrolithotomy, Percutaneous , Nephrostomy, Percutaneous , Humans , Middle Aged , Nephrolithotomy, Percutaneous/adverse effects , Nephrolithotomy, Percutaneous/methods , Retrospective Studies , Kidney Calculi/diagnostic imaging , Kidney Calculi/surgery , Kidney Calculi/etiology , Tomography, X-Ray Computed , Predictive Value of Tests , Treatment Outcome , Nephrostomy, Percutaneous/adverse effects , Nephrostomy, Percutaneous/methods
2.
J Physiol ; 587(Pt 12): 2775-82, 2009 Jun 15.
Article in English | MEDLINE | ID: mdl-19525562

ABSTRACT

A major problem of neurobiology concerns the failure of injured mammalian spinal cord to repair itself. This review summarizes work done on two preparations in which regeneration can occur: the central nervous system of an invertebrate, the leech, and the spinal cord of an immature mammal, the opossum. The aim is to understand cellular and molecular mechanisms that promote and prevent regeneration. In the leech, an individual axon regrows successfully to re-establish connections with its synaptic target, while avoiding other neurons. Functions that were lost are thereby restored. Moreover, pairs of identified neurons become re-connected with appropriate synapses in culture. It has been shown that microglial cells and nitric oxide play key roles in leech CNS regeneration. In the opossum, the neonatal brain and spinal cord are so tiny that they survive well in culture. Fibres grow across spinal cord lesions in neonatal animals and in vitro, but axon regeneration stops abruptly between postnatal days 9 and 12. A comprehensive search has been made in spinal cords that can and cannot regenerate to identify genes and establish their locations. At 9 days, growth-promoting genes, their receptors and key transcription molecules are up-regulated. By contrast at 12 days, growth-inhibitory molecules associated with myelin are prominent. The complete sequence of the opossum genome and new methods for transfecting genes offer ways to determine which molecules promote and which inhibit spinal cord regeneration. These results lead to questions about how basic research on mechanisms of regeneration could be 'translated' into effective therapies for patients with spinal cord injuries.


Subject(s)
Central Nervous System/physiology , Leeches/physiology , Nerve Regeneration/physiology , Opossums/physiology , Animals , Animals, Newborn , Gene Expression/physiology , Nerve Regeneration/genetics , Neural Pathways/physiology
3.
Neuroscience ; 150(4): 915-25, 2007 Dec 19.
Article in English | MEDLINE | ID: mdl-18031937

ABSTRACT

Regeneration of neuronal circuits cannot be successful without restoration of full function, including recovery of behavioral plasticity, which we have found is delayed after regeneration of specific synapses. Experiments were designed to measure neuronal changes that may underlie recovery of function. Sensitization of the leech withdrawal reflex is a non-associative form of learning that depends on the S-interneuron. Cutting an S-cell axon in Faivre's nerve disrupted the capacity for sensitization. The S-cell axon regenerated its electrical synapse with its homologous cell after 3-4 weeks, but the capacity for sensitization was delayed for an additional 2-3 weeks. In the present experiments another form of non-associative conditioning, dishabituation, was also eliminated by S-cell axotomy; it returned following regeneration. Semi-intact preparations were made for behavioral studies, and chains of ganglia with some skin were used for intracellular recording and skin stimulation. In both preparations there was a similar time-course, during 6 weeks, of a lesion-induced decrease and delayed restoration of both S-cell action potential threshold to depolarizing pulses and S-cell firing in response to test stimuli. However, the ability of sensitizing stimuli to decrease S-cell threshold and enhance S-cell activity in response to test stimuli did not fully return after regeneration, indicating that there were lasting changes in the circuit extending beyond the period necessary for full recovery of behavior. Intracellular recordings from the axotomized S-cell revealed a shift in the usual balance of excitatory and inhibitory input, with inhibition enhanced. These results indicate that loss of behavioral plasticity of reflexive shortening following axotomy in the S-cell chain may be related to reduced S-cell activity, and that additional processes underlie full recovery of sensitization of the whole body shortening reflex.


Subject(s)
Interneurons/cytology , Nerve Net/cytology , Nerve Regeneration/physiology , Neuronal Plasticity/physiology , Recovery of Function/physiology , Synapses/physiology , Action Potentials/physiology , Action Potentials/radiation effects , Animals , Axotomy/methods , Behavior, Animal , Dose-Response Relationship, Radiation , Electric Stimulation/methods , In Vitro Techniques , Interneurons/physiology , Leeches , Models, Neurological , Recovery of Function/radiation effects , Reflex/physiology , Synaptic Transmission/physiology , Time Factors
4.
Neuroscience ; 148(1): 65-81, 2007 Aug 10.
Article in English | MEDLINE | ID: mdl-17644266

ABSTRACT

The spatial and temporal patterns of action potential initiations were studied in a behaving leech preparation to determine the basis of increased firing that accompanies sensitization, a form of non-associative learning requiring the S-interneurons. Little is known at the network level about mechanisms of behavioral sensitization. The S-interneurons, one in each ganglion and linked by electrical synapses with both neighbors to form a chain, are interposed between sensory and motor neurons. In sensitized preparations the strength of shortening is related to S-cell firing, which itself is the result of impulses initiating in several S-cells. Because the S-cells, as independent initiation sites, all contribute to activity in the chain, it was hypothesized that during sensitization, increased multi-site activity increased the chain's firing rate. However, it was found that during sensitization, the single site with the largest initiation rate, the S-cell in the stimulated segment, suppressed initiations in adjacent ganglia. Experiments showed this was both because (1) it received the earliest, greatest input and (2) the delayed synaptic input to the adjacent S-cells coincided with the action potential refractory period. A compartmental model of the S-cell and its inputs showed that a simple, intrinsic mechanism of inexcitability after each action potential may account for suppression of impulse initiations. Thus, a non-synaptic competition between neurons alters synaptic integration in the chain. In one mode, inputs to different sites sum independently, whereas in another, synaptic input to a single site precisely specifies the overall pattern of activity.


Subject(s)
Action Potentials/physiology , Hirudo medicinalis/physiology , Interneurons/physiology , Learning/physiology , Nervous System Physiological Phenomena , Neural Pathways/physiology , Animals , Electric Stimulation , Electrical Synapses/physiology , Ganglia, Invertebrate/cytology , Ganglia, Invertebrate/physiology , Hirudo medicinalis/cytology , Interneurons/cytology , Nerve Net/cytology , Nerve Net/physiology , Neural Pathways/cytology , Neurons, Afferent/physiology , Reflex/physiology , Refractory Period, Electrophysiological/physiology , Synaptic Transmission/physiology
5.
Neuroscience ; 141(1): 223-31, 2006 Aug 11.
Article in English | MEDLINE | ID: mdl-16675136

ABSTRACT

In newborn and adult mammals, chemosensory drive exerted by CO(2) and H(+) provides an essential tonic input: without it the rhythm of respiration is abolished. It is not known, however, whether this chemosensory drive and the respiratory rhythm appear simultaneously during development. In isolated brainstem-spinal cord preparations from fetal mice, we determined at what stage of fetal life the respiratory rhythm appeared in third to fifth cervical ventral roots (phrenic motoneurons) and whether this fetal rhythm was sensitive to chemosensory inputs. A respiratory-like rhythm consisting of short duration bursts of discharges recurring at 2-16 min(-1) was detected in two of nine embryonic day 13 fetuses; it was abolished by transection of the spinal cord between the first to second cervical segments and was phase-related to rhythmic activity from medullary units of the ventral respiratory group. At embryonic day 13, it coexisted with a slow rhythm (0.1-2.0 min(-1)) of long duration bursts of action potentials which was generated by the spinal cord. At later fetal stages, the respiratory-like rhythm became more robust and of higher frequency, while the spinal cord rhythm became less obvious. At all fetal stages, acidification of the superfusion medium from pH 7.5-7.2 or 7.4-7.3 or 7.4 to 7.2 increased the frequency of both the respiratory-like and the spinal cord rhythms. In addition, acidification reduced the amplitude of the integrated burst activity of the spinal cord rhythm of embryonic day 13-embryonic day 16 fetuses and the respiratory-like rhythm of embryonic day 17 and older fetuses. Our results indicate that the rhythms transmitted by phrenic motoneurons during fetal development are chemosensitive from early fetal stages. Through its effects on induction and patterning of the rhythm, chemosensory drive may play a role in activity-dependent formation of respiratory neural networks.


Subject(s)
Motor Neurons/physiology , Periodicity , Respiratory Center/physiology , Spinal Cord/embryology , Action Potentials/physiology , Age Factors , Animals , Animals, Newborn , Brain Stem/cytology , Brain Stem/embryology , Embryo, Mammalian , Hydrogen-Ion Concentration , In Vitro Techniques , Mice , Spinal Cord/cytology , Spinal Cord Injuries/physiopathology , Statistics, Nonparametric , Stimulation, Chemical
6.
Neuroscience ; 137(4): 1221-7, 2006.
Article in English | MEDLINE | ID: mdl-16361062

ABSTRACT

Unfailing respiration depends on neural mechanisms already present in mammals before birth. Experiments were made to determine how inspiratory and expiratory neurons are grouped in the brainstem of fetal mice. A further aim was to assess whether rhythmicity arises from a single pacemaker or is generated by multiple sites in the brainstem. To measure neuronal firing, a fluorescent calcium indicator dye was applied to embryonic central nervous systems isolated from mice. While respiratory commands were monitored electrically from third to fifth cervical ventral roots, activity was measured optically over areas containing groups of respiratory neurones, or single neurones, along the medulla from the facial nucleus to the pre-Bötzinger complex. Large optical signals allowed recordings to be made during individual respiratory cycles. Inspiratory and expiratory neurones were intermingled. A novel finding was that bursts of activity arose in a discrete area intermittently, occurring during some breaths, but failing in others. Raised CO2 partial pressure or lowered pH increased the frequency of respiration; neurons then fired reliably with every cycle. Movies of activity revealed patterns of activation of inspiratory and expiratory neurones during successive respiratory cycles; there was no evidence for waves spreading systematically from region to region. Our results suggest that firing of neurons in immature respiratory circuits is a stochastic process, and that the rhythm does not depend on a single pacemaker. Respiratory circuits in fetal mouse brainstem appear to possess a high safety factor for generating rhythmicity, which may or may not persist as development proceeds.


Subject(s)
Brain Stem/embryology , Brain Stem/physiology , Medulla Oblongata/physiology , Respiratory System/embryology , Animals , Brain Mapping , Female , Medulla Oblongata/embryology , Mice , Models, Animal , Nerve Net , Pregnancy , Respiratory Mechanics/physiology
7.
J Membr Biol ; 206(3): 187-201, 2005 Aug.
Article in English | MEDLINE | ID: mdl-16456714

ABSTRACT

Electrotransfection and electrofusion, both widely used in research and medical applications, still have to face a range of problems, including the existence of electroporation-resistant cell types, cell mortality and also great batch-to-batch variations of the transfection and fusion yields. In the present study, a systematic analysis of the parameters critical for the efficiency and robustness of electromanipulation protocols was performed on five mammalian cell types. Factors examined included the sugar composition of hypotonic pulse media (trehalose, sorbitol or inositol), the kinetics of cell volume changes prior to electropulsing, as well as the growth medium additives used for post-pulse cell cultivation. Whereas the disaccharide trehalose generally allowed regulatory volume decrease (RVD), the monomeric sugar alcohols sorbitol and inositol inhibited RVD or even induced secondary swelling. The different volume responses could be explained by the sugar selectivity of volume-sensitive channels (VSC) in the plasma membrane of all tested cell types. Based on the volumetric data, highest transfection and fusion yields were mostly achieved when the target cells were exposed to hypotonicity for about 2 min prior to electropulsing. Longer hypotonic treatment (10-20 min) decreased the yields of viable transfected and hybrid cells due to (1) the cell size reduction upon RVD (trehalose) or (2) the excessive losses of cytosolic electrolytes through VSC (inositol/sorbitol). Doping the plasma membrane with lipophilic anions prevented both cell shrinkage and ion losses (probably due to VSC inhibition), which in turn resulted in increased transfection and fusion efficiencies.


Subject(s)
Carbohydrate Metabolism/physiology , Cell Culture Techniques/methods , Cell Survival/drug effects , Electroporation/methods , Fibroblasts/physiology , Kidney/physiology , Water-Electrolyte Balance/physiology , Animals , Cell Line , Cell Size/radiation effects , Electromagnetic Fields , Fibroblasts/radiation effects , Humans , Jurkat Cells , Kidney/radiation effects , Mice , Transfection/methods , Water-Electrolyte Balance/drug effects
8.
J Membr Biol ; 189(1): 45-54, 2002 Sep 01.
Article in English | MEDLINE | ID: mdl-12202951

ABSTRACT

The disaccharide trehalose is increasingly being used as a very efficient stabilizer of cells, membranes and macromolecules during cryo- and lyoconservation. Although extracellular trehalose can reduce cryo- and lyodamage to mammalian cells, the sugar is required on both sides of the plasma membrane for maximum protection efficiency. In the present study, mouse myeloma cells were loaded with the disaccharide by means of reversible electropermeabilization in isotonic trehalose-substituted medium, which contained 290 mM trehalose as the major solute. By using the membrane-impermeable fluorescent dye propidium iodide as the reporter molecule, optimum electropulsing conditions were found, at which most permeabilized cells survived and recovered (i.e., resealed) their original membrane integrity within a few minutes after electric treatment. Microscopic examination during the resealing phase revealed that electropulsed cells shrank gradually to about 60% of their original volume. The kinetics of the dye uptake and the volumetric response of cells to electropulsing were analyzed using a theoretical model that relates the observed cell volume changes to the solute transport across the transiently permeabilized cell membrane. From the best fit of the model to the experimental data, the intracellular trehalose concentration in electropulsed cells was estimated to be about 100 mM. This loading efficiency compares favorably to other methods currently used for intracellular trehalose delivery. The results presented here point toward application of the electropermeabilization technique for loading cells with membrane-impermeable bioprotectants, with far-reaching implications for cryo- and lyopreservation of rare and valuable mammalian cells and tissues.


Subject(s)
Electric Stimulation , Electrochemistry/methods , Electroporation/methods , Multiple Myeloma/pathology , Preservation, Biological/methods , Trehalose/administration & dosage , Animals , Cell Line , Cell Membrane Permeability , Cell Size , Cell Survival , Flow Cytometry/methods , Mice , Multiple Myeloma/physiopathology , Sensitivity and Specificity
9.
J Membr Biol ; 184(2): 161-70, 2001 Nov 15.
Article in English | MEDLINE | ID: mdl-11719852

ABSTRACT

Mouse myeloma cells were electropermeabilized by single square-wave electric pulses with amplitudes of up to approximately 150 kV/cm and durations of 10-100 nsec. The effects of the field intensity, pulse duration and medium conductivity on cell viability and field-induced uptake of molecules were analyzed by quantitative flow cytometry using the membrane-impermeable fluorescent dye propidium iodide as indicator molecule. Despite the extremely large field strengths, the majority of cells survived the exposure to ultra-short field pulses. The electrically induced dye uptake increased markedly with decreasing conductivity of the suspending medium. We assigned this phenomenon to the transient electrodeformation (stretching) force that assumes its maximum value if cells are suspended in low-conductivity media, i.e., if the external conductivity sigmae is smaller than that of the cytosol sigmai. The stretching force vanishes when sigmae is equal to or larger than sigmai. Due to their capability of delivering extremely large electric fields, the pulse power systems used here appear to be a promising tool for the electropermeabilization of very small cells and vesicles (including intracellular organelles, liposomes, etc.).


Subject(s)
Cell Membrane Permeability/physiology , Electric Conductivity , Electroporation/methods , Animals , Electric Stimulation/methods , Flow Cytometry/methods , Fluorometry/methods , Mice , Multiple Myeloma , Propidium/metabolism , Tumor Cells, Cultured
10.
J Neurophysiol ; 86(3): 1226-36, 2001 Sep.
Article in English | MEDLINE | ID: mdl-11535672

ABSTRACT

Sensory input to an individual interneuron or motoneuron typically evokes activity at a single site, the initial segment, so that firing rate reflects the balance of excitation and inhibition there. In a network of cells that are electrically coupled, a sensory input produced by appropriate, localized stimulation can cause impulses to be initiated in several places. An example in the leech is the chain of S cells, which are critical for sensitization of reflex responses to mechanosensory stimulation. S cells, one per segment, form an electrically coupled chain extending the entire length of the CNS. Each S cell receives input from mechanosensory neurons in that segment. Because impulses can arise in any S cell and can reliably propagate throughout the chain, all the S cells behave like a single neuron with multiple initiation sites. In the present experiments, well-defined stimuli applied to a small area of skin evoked mechanosensory action potentials that propagated centrally to several segments, producing S cell impulses in those segments. Following pressure to the skin, impulses arose first in the S cell of the same segment as the stimulus, followed by impulses in S cells in other segments. Often four or five separate initiation sites were observed. This timing of impulse initiation played an important role in increasing the frequency of firing. Impulses arising at different sites did not usually collide but added to the total firing rate of the chain. A computational model is presented to illustrate how mechanosensory neurons distribute the effects of a single sensory stimulus into spatially and temporally separated synaptic input. The model predicts that changes in impulse propagation in mechanosensory neurons can alter S cell frequency of firing by changing the number of initiation sites.


Subject(s)
Action Potentials/physiology , Models, Neurological , Neurons/physiology , Animals , Electrophysiology , Leeches , Mechanoreceptors/physiology , Physical Stimulation
11.
J Neurosci ; 21(4): 1401-12, 2001 Feb 15.
Article in English | MEDLINE | ID: mdl-11160412

ABSTRACT

In studies of the cellular basis of learning, much attention has focused on plasticity in synaptic transmission in terms of transmitter release and the number or responsiveness of neurotransmitter receptors. However, changes in postsynaptic excitability independent of receptors may also play an important role. Changes in excitability of a single interneuron in the leech, the S-cell, were measured during non-associative learning of the whole-body shortening reflex. This interneuron was chosen because it is known to be necessary for sensitization and full dishabituation of the shortening response. During sensitization, S-cell excitability increased, and this enhancement corresponded to facilitation of the shortening reflex and increased S-cell activity during the elicited response. During habituation training, there was a decrement in both the shortening reflex and the elicited S-cell activity, along with decreased S-cell excitability. Conversely, dishabituation facilitated both the shortening response and S-cell activity during shortening, with an accompanying increase in S-cell excitability. Bath application of 1-10 micrometer serotonin (5HT), a modulatory neurotransmitter that is critical for sensitization, for full dishabituation, and for associative learning, increased S-cell excitability. S-cell excitability also increased after stimulation of the serotonergic Retzius cells. However, focal application of serotonin onto the S-cell soma hyperpolarized the interneuron, and bath application of a lower dose of serotonin (0.1 micrometer) decreased excitability. The observed changes in postsynaptic excitability appear to contribute to non-associative learning, and modulatory neurotransmitters, such as serotonin, evidently help regulate excitability. Such changes in S-cell excitability may also be relevant for more complex, associative forms of learning.


Subject(s)
Interneurons/metabolism , Learning/physiology , Serotonin/metabolism , Animals , Behavior, Animal/drug effects , Behavior, Animal/physiology , Dose-Response Relationship, Drug , Electric Stimulation , Ganglia, Invertebrate/cytology , Ganglia, Invertebrate/drug effects , Ganglia, Invertebrate/physiology , Habituation, Psychophysiologic/physiology , In Vitro Techniques , Interneurons/cytology , Interneurons/drug effects , Learning/drug effects , Leeches , Membrane Potentials/drug effects , Microelectrodes , Neuronal Plasticity/drug effects , Neuronal Plasticity/physiology , Reflex/drug effects , Reflex/physiology , Serotonin/pharmacology
12.
J Neurosci ; 21(1): 215-20, 2001 Jan 01.
Article in English | MEDLINE | ID: mdl-11150338

ABSTRACT

An early step in repair of the leech CNS is the appearance of endothelial nitric oxide synthase (eNOS) immunoreactivity and NOS activity, but coincident generation of NO at the lesion after injury has not been shown. This is important because NO can regulate microglial cell motility and axon growth. Indirect measurement of NO with the standard citrulline assay demonstrated that NO was generated within 30 min after nerve cord injury. A polarographic NO-selective self-referencing microelectrode that measures NO flux noninvasively was developed to obtain higher spatial and temporal resolution. With this probe, it was possible to demonstrate that immediately after the leech CNS was injured, NO left the lesion with a mean peak efflux of 803 +/- 99 fmol NO cm(-2) sec(-1). NO efflux exponentially declined to a constant value, as described through the equation f(t) = y(o) + ae(-t/tau), with tau = 117 +/- 30 sec. The constant y(o) = 15.8 +/- 4.5 fmol cm(-2) represents a sustained efflux of NO. Approximately 200 pmol NO cm(-2) is produced at the lesion (n = 8). Thus, injury activates eNOS already present in the CNS and precedes the accumulation of microglia at the lesion, consistent with the hypothesis that NO acts to stop the migrating microglia at the lesion site.


Subject(s)
Central Nervous System/physiology , Microelectrodes , Nerve Regeneration/physiology , Neurons/metabolism , Nitric Oxide/metabolism , Animals , Central Nervous System/chemistry , Citrulline/metabolism , Leeches , Microglia/cytology , NG-Nitroarginine Methyl Ester/pharmacology , Nerve Crush , Nitric Oxide/analysis , Nitric Oxide Synthase/antagonists & inhibitors , Nitric Oxide Synthase/metabolism , Nitric Oxide Synthase Type III , Polarography/instrumentation
13.
Genetics ; 154(3): 1335-46, 2000 Mar.
Article in English | MEDLINE | ID: mdl-10757774

ABSTRACT

Two groups of mutants that affect the morphology of the lemma, a floral bract of barley, are described. The first comprises phenotypes associated with mutant alleles of calcaroides loci. On the lemma of these mutants, a well-organized neomorphic structure is formed, termed the sac. We provide a morphological description of wild-type (WT) and mutant lemmas, based on scanning electron microscopy (SEM), showing that both consist of similar tissues, but that the mutant is characterized by reversed growth polarity. The sac is a unique structure among grasses, and it is remarkable that recessive mutations at five different genetic loci lead to the same organ. The second group of mutants carry recessive alleles of two leafy lemma genes, both of which are necessary to cause the transformation of the lemma into a structure having all characteristics of a vegetative leaf, as shown by SEM analysis. The presence of sheath, blade, and ligule in the mutant lemma suggests that wild-type lemma development is interrupted at a leaf-like stage. The genes cal a, b, C, d, 23, lel1, and lel2 have now been mapped at precise positions on linkage groups 2, 7, 7, 3, 7, 5, and 7, respectively. The mutants considered in this article are unaffected in other floral organs. A model for lemma development is suggested.


Subject(s)
Hordeum/genetics , Mutation
14.
J Neurophysiol ; 83(3): 1693-700, 2000 Mar.
Article in English | MEDLINE | ID: mdl-10712489

ABSTRACT

In leech mechanosensory neurons, action potentials reverse direction, or reflect, at central branch points. This process enhances synaptic transmission from individual axon branches by rapidly activating synapses twice, thereby producing facilitation. At the same branch points action potentials may fail to propagate, which can reduce transmission. It is now shown that presynaptic action potential reflection and failure under physiological conditions influence transmission to the same postsynaptic neuron, the S cell. The S cell is an interneuron essential for a form of nonassociative learning, sensitization of the whole body shortening reflex. The P to S synapse has components that appear monosynaptic (termed "direct") and polysynaptic, both with glutamatergic pharmacology. Reflection at P cell branch points on average doubled transmission to the S cell, whereas action potential failure, or conduction block, at the same branch points decreased it by one-half. Each of two different branch points affected transmission, indicating that the P to S connection is spatially distributed around these branch points. This was confirmed by examining the locations of individual contacts made by the P cell with the S cell and its electrically coupled partner C cells. These results show that presynaptic neuronal morphology produces a range of transmission states at a set of synapses onto a neuron necessary for a form of learning. Reflection and conduction block are activity-dependent and are basic properties of action potential propagation that have been seen in other systems, including axons and dendrites in the mammalian brain. Individual branch points and the distribution of synapses around those branch points can substantially influence neuronal transmission and plasticity.


Subject(s)
Axons/physiology , Excitatory Postsynaptic Potentials/physiology , Learning/physiology , Leeches/physiology , Neurons/physiology , Action Potentials/physiology , Animals , Axons/ultrastructure , Axotomy , Fluorescent Dyes , Isoquinolines , Mechanoreceptors/physiology , Microscopy, Electron , Neural Conduction/physiology , Neurons/ultrastructure , Pressure , Reflex, Monosynaptic/physiology , Synaptic Transmission/physiology
15.
J Neurosci ; 20(3): 1036-43, 2000 Feb 01.
Article in English | MEDLINE | ID: mdl-10648709

ABSTRACT

Damage to the leech or mammalian CNS increases nitric oxide (NO) production and causes accumulation of phagocytic microglial cells at the injury site. The aim of this study was to determine whether NO plays a role in microglial migration and accumulation at lesions in which NO is generated by a rapidly appearing endothelial nitric oxide synthase (eNOS) in leeches. Immunohistochemistry and cytochemistry demonstrated active eNOS before and throughout the period of microglial accumulation at the lesion. Decreasing NO synthesis by application of the NOS inhibitor N(w)-nitro-L-arginine methyl ester (1 mM) significantly reduced microglial accumulation, whereas its inactive enantiomer N(w)-nitro-D-arginine methyl ester (1 mM) resulted in microglial accumulation similar to that in crushed controls. Increasing NO with the donor spermine NONOate (SPNO) (1 mM) also inhibited accumulation, but not in the presence of the NO scavenger 2-(4-carboxyphenyl)-4,4,5, 5-teramethylimidazoline-oxyl-3-oxide (50 microM). The effect of SPNO was reversed by washout. SPNO application reduced average microglial migratory speeds and even reversibly arrested cell movement, as measured in living nerve cords. These results suggest that NO produced at a lesion may be a stop signal for microglia to accumulate there and that it can act on microglia early in their migration. Thus, NO may assume a larger role in nerve repair and recovery from injury by modulating accumulation of microglia, which appear to be important for axonal regeneration.


Subject(s)
Central Nervous System/injuries , Microglia/pathology , Microglia/physiology , Nitric Oxide/physiology , Wounds and Injuries/pathology , Wounds and Injuries/physiopathology , Animals , Cell Movement/drug effects , Enzyme Inhibitors/pharmacology , Leeches , Microglia/drug effects , NG-Nitroarginine Methyl Ester/pharmacology , Nerve Crush , Nitric Oxide Donors/pharmacology , Nitric Oxide Synthase/antagonists & inhibitors , Nitrogen Oxides , Spermine/analogs & derivatives , Spermine/pharmacology
16.
Genetics ; 149(4): 2039-56, 1998 Aug.
Article in English | MEDLINE | ID: mdl-9691056

ABSTRACT

A strategy based upon AFLP markers for high-efficiency mapping of morphological mutations and DNA probes to linkage groups in barley is presented. First, 511 AFLP markers were placed on the linkage map derived from the cross Proctor x Nudinka. Second, loci controlling phenotypic traits were assigned to linkage groups by AFLP analysis, using F2 populations consisting of 30-50 mutant plants derived from crosses of the type "mutant x Proctor" and "mutant x Nudinka." To map DNA probes, 67 different wild-type barley lines were selected to generate F2 populations by crossing with Proctor and Nudinka. F2 plants that were polymorphic for a given RFLP fragment were classified into genotypic classes. Linkage of the RFLP polymorphism to 1 of the 511 AFLP loci was indicated by cosegregation. The use of the strategy is exemplified by the mapping of the mutation branched-5 to chromosome 2 and of the DNA probes Bkn2 and BM-7 to chromosomes 5 and 1, respectively. Map expansion and marker order in map regions with dense clustering of markers represented a particular problem. A discussion considering the effect of noncanonical recombinant products on these two parameters is provided.


Subject(s)
Chromosome Mapping/methods , DNA Probes , Hordeum/genetics , Mutation , Alleles , Base Sequence , Crosses, Genetic , DNA Primers/genetics , Genes, Plant , Genetic Linkage , Phenotype , Polymorphism, Genetic , Polymorphism, Restriction Fragment Length
17.
Proc Biol Sci ; 265(1411): 2171-5, 1998 Nov 22.
Article in English | MEDLINE | ID: mdl-9872006

ABSTRACT

It is known that nitric oxide (NO) is produced by injured tissues of the mammalian central nervous system (CNS) within days of injury. The aim of the present experiments was to determine the cellular synthesis of NO in the CNS immediately after injury, using the CNS of the leech which is capable of synapse regeneration, as a step towards understanding the role of NO in nerve repair. We report that within minutes after crushing the nerve cord of the leech, the region of damage stained histochemically for NADPH diaphorase, which is indicative of nitric oxide synthase (NOS) activity, and was immunoreactive for endothelial NOS (eNOS). On immunoblots of leech CNS extract, the same antibody detected a band with a relative molecular mass of 140,000, which is approximately the size of vertebrate eNOS. Cells expressing eNOS immunoreactivity as a result of injury were identified after freezing nerve cords, a procedure that produced less tissue distortion than mechanical crushing. Immunoreactive cells included connective glia and some microglia. Calmodulin was necessary for the eNOS immunoreactivity: it was blocked by calmodulin antagonist W7 (25 microM), but not by similar concentrations of the less potent calmodulin antagonist W12. Thus in the leech CNS, in which axon and synapse regeneration is successful, an increase in NOS activity at lesions appears to be among the earliest responses to injury and may be important for repair of axons.


Subject(s)
Gene Expression Regulation, Enzymologic , Microglia/enzymology , Nervous System/enzymology , Neuroglia/enzymology , Nitric Oxide Synthase/metabolism , Animals , Calmodulin/antagonists & inhibitors , Freezing , Immunohistochemistry , Leeches , Microglia/physiology , NADPH Dehydrogenase/analysis , Nerve Crush , Nerve Regeneration , Neuroglia/physiology , Nitric Oxide Synthase/biosynthesis , Nitric Oxide Synthase Type III , Sulfonamides/pharmacology , Synapses/physiology , Trauma, Nervous System
18.
J Neurosci ; 17(16): 6478-82, 1997 Aug 15.
Article in English | MEDLINE | ID: mdl-9236255

ABSTRACT

Sensitization is a form of nonassociative learning in which a strong or noxious stimulus persistently enhances the response produced by a weaker stimulus. In the leech Hirudo medicinalis, the S-interneuron is required for sensitization of the shortening response. A single S-cell axon was surgically separated from its sole synaptic partner, the neighboring S-cell. This consistently eliminated sensitization without impairing reflexive shortening itself, as measured in semi-intact specimens. Sensitization of the shortening reflex returned after 3 weeks when the severed axon grew and regenerated its specific electrical synapse within the nerve cord, as shown by restored conduction of impulses between S-cells. This confirms the essential role of one neuron, the S-cell, in sensitization, and it demonstrates that regeneration of the synapse between S-cells restores this example of nonassociative learning.


Subject(s)
Conditioning, Psychological/physiology , Nerve Regeneration/physiology , Synapses/physiology , Animals , Axons/physiology , Behavior, Animal/physiology , Denervation , Electrophysiology , Ganglia, Invertebrate/cytology , Ganglia, Invertebrate/physiology , Ganglia, Invertebrate/surgery , Leeches , Neurons, Afferent/physiology , Neurons, Afferent/ultrastructure , Sensitivity and Specificity
19.
J Neurosci ; 16(8): 2585-91, 1996 Apr 15.
Article in English | MEDLINE | ID: mdl-8786434

ABSTRACT

The initiation site in neurons is where the excitatory and inhibitory inputs sum to generate action potentials. It is generally considered to be at a fixed location, typically at the axon hillock or initial segment, although action potentials, or impulses, could in theory arise at a site that shifts dynamically. The data reported here show that the initiation site can shift in a graded manner, by as much as 175 microm, depending on the level of neuronal excitation. Laser axotomy reveals that the Anterior Pagoda (AP) neuron of the leech is excitable within the synaptic neuropil before its axon bifurcates. Using an electrophysiological technique to measure relative delays in impulses arriving at different sites, we have found that depolarization, either by applied current or by synaptic input, can shift the site of impulse initiation in the cell proximally toward the soma and neurites receiving synaptic input. Impulse initiation in this region should enhance the efficacy of inputs synapsing there. Conversely, hyperpolarization can shift the initiation site distally. A shifting initiation site, therefore, may be a mechanism by which synaptic inputs can rapidly enhance or suppress the active response of the AP neuron to other synaptic inputs.


Subject(s)
Action Potentials/physiology , Neurons/physiology , Animals , Leeches , Presynaptic Terminals/physiology , Time Factors
20.
J Comp Neurol ; 366(4): 600-12, 1996 Mar 18.
Article in English | MEDLINE | ID: mdl-8833112

ABSTRACT

The aim of these experiments was to analyze neurite outgrowth during regeneration of opossum spinal cord isolated from Monodelfis domestica and maintained in culture for 3-5 days. Lesions were made by crushing with forceps. In isolated spinal cords of animals aged 3 days, neurites entered the crush and grew along the basal lamina of the pia mater. Growth cones with pleiomorphic appearance containing vesicles, mitochondria and microtubules were abundant in the marginal zone, as were synaptoid contacts with active zones facing basal lamina. In preparations from animals aged 11-12 days, the lesion site was disrupted and contained only degenerating axons, debris and vesicles. Axons and growth cones entered the edge of the lesion but did not extend into it. Lesions in young animals extended over distances of more than 1 mm and contained no radial glia. The damaged area in older preparations was restricted to the crush site with normal astrocytes, oligodendrocytes and neurons immediately adjacent to the lesion. Thus, similar crushes produced more extensive damage in younger spinal cords that were capable of regeneration than in older cords that were not. Dorsal root ganglion fibers labeled with carbocyanine dye (DiI) were observed by video imaging as they grew through lesions. Individual growth cones examined subsequently by electron microscopy had grown again along pial basal lamina. After 5 days in culture dorsal root stimulation gave rise to discharges in ventral roots beyond the lesion indicating that synaptic connections were formed by growing fibers.


Subject(s)
Nerve Fibers/physiology , Nerve Regeneration/physiology , Neurites/physiology , Spinal Cord Injuries/physiopathology , Animals , Animals, Newborn , Axons/physiology , Cells, Cultured , Electric Stimulation , Ganglia, Spinal/cytology , Ganglia, Spinal/physiology , Nerve Crush , Neurons/physiology , Spinal Cord Injuries/pathology , Synapses/physiology , Video Recording
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