Role of nitric oxide in the remodelling of extracellular matrix in myxomatous mitral valve degeneration of dogs and pigs

Myxomatous mitral valve degeneration (MMVD) or endocardiosis is a heart valve disease that occurs in many mammalian species, especially in humans, dogs and pigs. Nitric oxide (NO) plays an important role in the MMVD development. NO can be indirectly evaluated by the nitric-oxide synthase (NOS) expression and by the histochemical reaction of nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d). The aim of this study was to evaluate NOS activity, by NADPH-d reaction, in the anterior leaflet of dogs with regular mitral valves and in those with MMVD, as well as in young swine and old females, comparing the reaction level with the degree of endocardiosis disease and also the histological alterations. Twelve mitral valves of dogs and 22 of swine were used for the research. All the valves were macroscopically analyzed for the occurrence or not of endocardiosis. They were fixed in a 4% paraformaldehyde, exposed to NADPH-d reaction, routinely processed and microscopically evaluated for the detection of mucopolysaccharides (MPS) deposition, collagen degeneration, fibrosis and level of endocardiosis. In dogs, relation was observed between higher intensity of the NADPH-d reaction, higher endocardiosis degree, MPS deposition as well as the collagen degeneration. No alteration in color was observed in pigs´ valves during NADPH-d reaction. In conclusion, NO works in canine mitral

Histopathological pattern of exfoliative and ulcerative skin lesions in dogs with leishmaniasis / Padrões histopatológicos das lesões descamativas e ulcerativas da pele em cães com leishmaniose

Canine visceral leishmaniasis is an endemic infectious disease in some São Paulo state regions and even though it is a systemic disease, in the dog, the main clinical sign is dermatological. Thirty dogs with positive results in serology and parasitological exams for leishmaniasis from Araçatuba city were evaluated. They just showed dermatological signs and were divided in two groups, one with exfoliative (n=1 ) and other with ulcerative (n=1 ) lesions. Histopathological patterns in the group of exfoliative lesions were: periadnexial dermatitis (5/15, 33,3%), superficial perivascular dermatitis (1/15, 6,6%), nodular dermatitis (1/1 , , %) and mixed dermatitis (8/1 , , %), including intersticial/periadnexial dermatitis (1/8, 12,5%), lichenoid/perivascular superficial and deep dermatitis (1/8, 12,5%), perivascular superficial and deep/periadnexial dermatitis (1/8, 12,5%) and superficial perivascular/perianexial dermatitis ( /8, , %). In the group of ulcerative lesions, the histopathological patterns were: perivascular superficial and deep dermatitis (5/15, 33,3%), diffuse dermatitis (3/15, 20%), periadnexial dermatitis ( /1 , 1 , %), nodular dermatitis (1/1 , , %) and mixed dermatitis ( /1 , , %), including intersticial/ perivascular superficial and deep dermatitis (1/4, 25%), nodular/periadnexial dermatitis (1/4, 25%), fibrosing/perianexial dermatitis (1/4, 25%) and perivascular

A leishmaniose visceral canina é uma doença infecciosa crônica considerada endêmica em algumas regiões do estado de São Paulo e, apesar de ser uma enfermidade sistêmica, no cão, a maioria dos sinais clínicos são dermatológicos. Foram avaliados trinta cães com diagnóstico de leishmaniose, da região de Araçatuba, pelos exames parasitológico e sorológico. Os cães apresentando apenas sinais dermatológicos foram divididos em dois grupos: um grupo com descamação e outro com ulceração. Os padrões histopatológicos da pele encontrados nos cães com lesões descamativas (n=15) foram de dermatite perianexial (5/15, 33,3%), dermatite perivascular superficial (1/15, 6,6%), dermatite nodular (1/15, 6,6%), e dentre as dermatites mistas (8/15, 53,3%), dermatite intersticial/perianexial (1/8, 12,5%), dermatite liquenóide/perivascular superficial e profunda (1/8, 12,5%), dermatite perivascular superficial e profunda/perianexial (1/8, 12,5%) e dermatite perivascular superficial/perianexial (5/8, 62,5%). Os cães com lesões ulcerativas (n=15) apresentaram padrões histopatológicos de dermatite perivascular superficial e profunda (5/15, 33,3%), dermatite difusa (3/15, 20%), dermatite perianexial (2/15, 13,3%), dermatite nodular (1/15, 6,6%), e dentre as dermatites mistas (4/15, 26,6%), dermatite intersticial/perivascular superficial e profunda (1/4, 25%), dermatite nodular/perianexial (1/4, 25%), derma

Histopathological pattern of exfoliative and ulcerative skin lesions in dogs with leishmaniasis / Padrões histopatológicos das lesões descamativas e ulcerativas da pele em cães com leishmaniose

Canine visceral leishmaniasis is an endemic infectious disease in some São Paulo state regions and even though it is a systemic disease, in the dog, the main clinical sign is dermatological. Thirty dogs with positive results in serology and parasitological exams for leishmaniasis from Araçatuba city were evaluated. They just showed dermatological signs and were divided in two groups, one with exfoliative (n=1 ) and other with ulcerative (n=1 ) lesions. Histopathological patterns in the group of exfoliative lesions were: periadnexial dermatitis (5/15, 33,3%), superficial perivascular dermatitis (1/15, 6,6%), nodular dermatitis (1/1 , , %) and mixed dermatitis (8/1 , , %), including intersticial/periadnexial dermatitis (1/8, 12,5%), lichenoid/perivascular superficial and deep dermatitis (1/8, 12,5%), perivascular superficial and deep/periadnexial dermatitis (1/8, 12,5%) and superficial perivascular/perianexial dermatitis ( /8, , %). In the group of ulcerative lesions, the histopathological patterns were: perivascular superficial and deep dermatitis (5/15, 33,3%), diffuse dermatitis (3/15, 20%), periadnexial dermatitis ( /1 , 1 , %), nodular dermatitis (1/1 , , %) and mixed dermatitis ( /1 , , %), including intersticial/ perivascular superficial and deep dermatitis (1/4, 25%), nodular/periadnexial dermatitis (1/4, 25%), fibrosing/perianexial dermatitis (1/4, 25%) and perivascular

A leishmaniose visceral canina é uma doença infecciosa crônica considerada endêmica em algumas regiões do estado de São Paulo e, apesar de ser uma enfermidade sistêmica, no cão, a maioria dos sinais clínicos são dermatológicos. Foram avaliados trinta cães com diagnóstico de leishmaniose, da região de Araçatuba, pelos exames parasitológico e sorológico. Os cães apresentando apenas sinais dermatológicos foram divididos em dois grupos: um grupo com descamação e outro com ulceração. Os padrões histopatológicos da pele encontrados nos cães com lesões descamativas (n=15) foram de dermatite perianexial (5/15, 33,3%), dermatite perivascular superficial (1/15, 6,6%), dermatite nodular (1/15, 6,6%), e dentre as dermatites mistas (8/15, 53,3%), dermatite intersticial/perianexial (1/8, 12,5%), dermatite liquenóide/perivascular superficial e profunda (1/8, 12,5%), dermatite perivascular superficial e profunda/perianexial (1/8, 12,5%) e dermatite perivascular superficial/perianexial (5/8, 62,5%). Os cães com lesões ulcerativas (n=15) apresentaram padrões histopatológicos de dermatite perivascular superficial e profunda (5/15, 33,3%), dermatite difusa (3/15, 20%), dermatite perianexial (2/15, 13,3%), dermatite nodular (1/15, 6,6%), e dentre as dermatites mistas (4/15, 26,6%), dermatite intersticial/perivascular superficial e profunda (1/4, 25%), dermatite nodular/perianexial (1/4, 25%), derma