Benthic harmful microalgae and their impacts in South America.

Public awareness about Benthic Harmful Algal Blooms (BHABs) and their negative impacts has increased substantially over the past few decades. Even so, reports of BHABs remain relatively scarce in South America (SA). This paper provides a comprehensive overview of the current state of knowledge on BHABs in the continent, by integrating data from published articles, books, and technical reports. We recorded ∼300 different occurrences of potentially toxic BHAB species over the Caribbean, Atlantic and Pacific coasts, mostly in marine (>95%) but also in estuarine areas located from 12°36' N to 54°53' S. Over 70% of the data was published/released within the past 10 years, and ∼85% were concentrated in Brazil, Venezuela, Ecuador and Colombia. Benthic species were mainly associated with macroalgae, seagrass and sediment. Incidental detection in the plankton was also relevant, mainly in places where studies targeting BHAB species are still rare, like Argentina, Uruguay, Chile and Peru. The study listed 31 infrageneric taxa of potentially toxic benthic dinoflagellates and eight of estuarine cyanobacteria occurring in SA, with the greatest species diversity recorded in the equatorial-tropical zone, mainly in northeastern Brazil (Atlantic), Venezuela and Colombia (Caribbean), and the Galapagos Islands, Ecuador (Pacific). Local strains of Amphidinium, Gambierdiscus, Coolia and Prorocentrum spp. produced toxic compounds of emerging concern. Prorocentrum lima species complex was the most common and widely distributed taxon, followed by Ostreopsis cf. ovata. In fact, these two dinoflagellates were associated with most BHAB events in SA. Whereas the former has caused the contamination of multiple marine organisms and cases of Diarrhetic Shellfish Poisoning in subtropical and temperate areas, the latter has been associated with faunal mortalities and is suspected of causing respiratory illness to beach users in tropical places. Ciguatera Poisoning has been reported in Colombia (∼240 cases; no deaths) and Venezuela (60 cases; two deaths), and may be also a risk in other places where Gambierdiscus spp. and Fukuyoa paulensis have been reported, such as the Galapagos Islands and the tropical Brazilian coast. Despite the recent advances, negative impacts from BHABs in SA are intensified by limited research/training funding, as well as the lack of official HAB monitoring and poor analytical capability for species identification and toxin detection in parts of the continent.

Morphology and phylogeny of Prorocentrum porosum sp. nov. (Dinophyceae): A new benthic toxic dinoflagellate from the Atlantic and Pacific Oceans.

A new marine benthic toxic Prorocentrum species is described from the tropical/subtropical regions of the Atlantic (Colombian Caribbean Sea and Northeast Brazil) and Pacific (Southern Japan) oceans. Morphological cell structures were examined using light (LM) and scanning electron (SEM) microscopy. Prorocentrum porosum sp. nov. was characterized by 35.9-50.2 µm long and 25.4-45.7 µm deep cells, covered by broadly ovoid symmetric thecal plates. The surface of both thecal plates is smooth and covered by randomly scattered kidney-shaped pores (n = 102-149), rounder towards the center, absent in the central part, and surrounded by a conspicuous marginal ring of about 69-92 evenly spaced pores. Broad V-shaped periflagellar area exhibiting flagellar and accessory pores. The molecular phylogenetic position of P. porosum sp. nov. was inferred using partial LSU rRNA gene (rDNA) and rDNA ITS sequences. This new species branched with high support in a Prorocentrum clade including P. caipirignum, P. hoffmannianum and P. cf. lima (P. lima morphotype 5 sensuZhang et al., 2015). Pairwise comparison of ITS1 and ITS2 transcripts with these closest relatives revealed the presence of compensatory base changes (CBCs), with the exception of P. cf. lima (P. lima morphotype 5), which only showed in ITS2 a hemi-CBC (HCBC) and two base changes that possibly induce a structural modification. Toxin analyses performed in two Colombian and Brazilian strains in the present study detected the presence of low amounts of okadaic acid.

Morphoanatomic variation in tissues of Rhizophora mangle seedlings subjected to different saline regimes: cross-seeding experiment.

Rhizophora mangle, one of the main neotropic mangrove species, has wide phenological variability associated with soil salinity. Since global warming is one of the main drivers of changes in salinity, understanding the influence of this variable at the species level would help improve the prediction of climate change in the ecological services provided by mangroves. To understand the physiological and/or anatomical responses to water stress generated by edaphic salinity and its relationship with phenological and structural diversity, we quantified the functional traits of leaf tissue subjected to a cross-seeding experiment between two forests with different ranges of natural salinity (0-18 PSU and 20 to 70 PSU). A total of 180 propagules, 90 native and 90 from the other forest, were planted in each forest. Every three months for a year, soil salinity and growth, adaptability, and survival of propagules that were transformed into seedlings were measured. The traits evaluated between the two saline regimes presented significant differences, as stated in the working hypothesis. Likewise, there were modifications in the hypodermis and the xylem vessels in the exchanged seedlings, tissues related to water storage, and conduction. These responses allowed native euhaline forest seedlings to grow in oligohaline. The opposite occurred with seedlings originating in low salinities that did not survive in high salinities. Differences in adaptability between populations of R. mangle subjected to ranges of contrasting salinity may imply changes at the structural level, zoning, and abundance of the species front to the climate change processes.

The Latin America and Caribbean HAB status report based on OBIS and HAEDAT maps and databases.

Harmful Algae Blooms (HAB) have been documented for at least fifty years in Latin America and the Caribbean (LAC), however, their impacts at social, ecological and economic levels are still little known. To contribute to the impact assessment of HABs in LAC region, the available information in HAEDAT, OBIS, CAREC, and CARPHA databases, and scientific literature was analyzed. This historical analysis allows identification of the main syndromes and causal organisms. Considering the existence of two regional working groups of the Intergovernmental Oceanographic Commission (IOC): Algas Nocivas del Caribe (ANCA) and Floraciones Algales Nocivas en Sudamérica (FANSA), representing Central American/Caribbean and South American countries, respectively, the analysis is presented both globally and subregional. For the FANSA region, the HAEDAT data base listed 249 records from 1970 to 2019, with a total of 1432 human intoxications, including 37 fatalities. The majority of these events comprised Paralytic Shellfish Toxins (49%), Diarrhetic Shellfish Toxins (34%), Cyanotoxins (12%) and 6 % other toxins. The total number of harmful taxa in the OBIS database includes 79 species distributed over 25 genera. The most commonly reported species are Alexandrium catenella/tamarense, Gymnodinium catenatum and the Dinophysis acuminata complex. Two new species Prorocentrum caipirignum Fraga, Menezes and Nascimento and Alexandrium fragae Branco and Menezes were newly described from Brazilian waters. In the ANCA region, HAEDAT listed 131 records from 1956 to 2018. The main problems are PSP and Ciguatera and common HAB taxa are Gambierdiscus, Gymnodinium, Pyrodinium, Alexandrium and Dinophysis. The most reported HAB forming species are Gymnodinium catenatum, Pyrodinium bahamense and Gambierdiscus spp. In recent years Margalefidinium polykrikoides blooms have become frequent, causing fish and invertebrates massive mortalities and impacts on touristic activities. In the LAC region, the greatest economic losses were produced by ichthyotoxic massive events causing salmon deaths associated to Pseudochattonella verruculosa and Alexandrium catenella in Chile and tuna deaths related to Tripos furca and Chattonella spp. in the Mexican Pacific. In the last decade, several studies in LAC have linked HAB events with local mesoscale oceanographic and atmospheric phenomena. Trends analyzed up to 2019 are related to the increasing awareness about presence of toxic species, the geographical expansion of already known species, the detection of new toxins for the region, and HAB events duration and/or impacts.

Stable-isotope analysis reveals sources of organic matter and ontogenic feeding shifts of a mangrove-dependent predator species, New Granada sea catfish, Ariopsis canteri.

To gain a better understanding on the trophic ecology of New Granada sea catfish, Ariopsis canteri, and their linkage to mangroves, nitrogen and stable carbon isotopes (δ15 N and δ13 C), as well as Bayesian mixing models, were used to explore trophic dynamics and potential ontogenic feeding shifts across different size classes: class I (8-20 cm), class II (21-32 cm) and class III (>32 cm). The study area was the estuary of the Atrato River Delta, where information about fish ecology is scarce. The δ13 C of size class I was lower (mean ± s.d. = -24.96 ± 0.69‰) than that of size classes II (-22.20 ± 0.90‰) and III (-22.00 ± 1.96‰). The δ15 N of size class I was lower (mean ± s.d. = 8.50 ± 0.67‰) than that of size classes II (9.77 ± 0.60‰) and III (10.00 ± 0.66‰). Body size was positively and significantly correlated to δ15 N and δ13 C. Individuals with LT > 32 cm presented the highest estimated trophic position (3.8). Five-source mixing models indicated that for class I, the mean estimated contribution of macroalgae was the highest (6%-57% c.i.), and for classes II and III, the mean estimated contribution of macrophytes was the highest (3%-53% c.i. and 4%-53% c.i., respectively). Ontogenetic feeding shifts of A. canteri were confirmed evidencing decreasing intraspecific competition between small and large individuals. Results suggest that mangroves are a nursery and feeding ground habitat for this species and that mangroves support A. canteri mainly due to the substrate/habitat that supports sources in the food webs. These results can be used in ecosystem-based fishery management focused on the protection of extensive mangrove areas in the southern Caribbean Sea.

Structural variation of potentially toxic epiphytic dinoflagellates on Thalassia testudinum from two coastal systems of Colombian Caribbean.

Some benthic dinoflagellates produce toxins that can affect other organisms including humans, and their proliferation seems to be related to the environmental variability. For this reason, the present study aims to compare the structural variation of potentially toxic dinoflagellates associated with the seagrass Thalassia testudinum from two nearby systems, with different environmental characteristics in Colombian Caribbean, corresponding to a brackish water coastal lagoon and an adjacent bay. Between January 2014 and December 2015, leaves of T. testudinum were collected monthly to obtain the dinoflagellates. Salinity, temperature, dissolved oxygen, pH, nutrients and total suspended solids (TSS) were measured, and precipitation data and the Oceanic Niño Index (ONI) were obtained. Dinoflagellates were detached from the leaves, morphologically identified by analyzing their thecal plates arrangements, and quantified using a Sedgewick-Rafter chamber. The information was analyzed using standard statistics and regression models. Fourteen species of potentially toxic epiphytic dinoflagellate belonging to four genera were recorded, being Prorocentrum the most representative in number of species. The maximum density, dominated by P. lima, were found in Bahía Chengue during the rainy season of 2014 (18452 and 20109 cells g-1 w.w.), with salinity of 35.50, high temperatures (>29.60 °C), dissolved oxygen >6 mg L-1, pH close to 8 and TSS >85 mg L-1. Densities at the Lagoon were lower than 80 cells g-1 w.w. with the highest values of Prorocentrum sp.1 under different environmental conditions. With the statistical relationships between the most abundant species and the main environmental variables, fundamental niche models were proposed in which cells could proliferate. The degree of risk to human health due to the presence of these potentially toxic epiphytic dinoflagellates will not be resolved until their toxicity discarded.

PARÁMETROS ESTRUCTURALES DE DOS POBLACIONES DE Crassostrea rhizophorae (OSTREIDAE) EN BAHÍA CISPATÁ, CARIBE COLOMBIANO / Structural Parameters of Two Populations of Crassostrea rhizophorae (Ostreidae) in Bahía Cispatá, Colombian Caribbean

RESUMEN La ostra del mangle es una especie de gran importancia ecológica y comercial en muchos lugares del Caribe. En Bahía de Cispatá, Colombia, no obstante, representa el sustento económico de un importante grupo de pescadores se carece de planes de manejo que regulen su extracción. El objetivo del presente estudio fue el de establecer la estructura poblacional de Crassostrea rhizophorae en Bahía Cispatá, como insumo básico para la formulación futura de planes de manejo de la especie. Debido a las diferencias fenotípicas observadas entre los individuos de los lugares muestreados, se obtuvieron secuencias del gen COI, concluyendo que todos los individuos corresponden a la especie Crassostrea rhizophorae. Con base en las características morfológicas se diferenciaron dos poblaciones, de las que no se evidenció coexistencia en una misma estación de muestreo. A través del programa FiSAT se evaluaron los principales parámetros poblacionales, encontrando que los individuos de la población con concha delgada crecen a mayor velocidad alcanzando mayores tamaños que los individuos de la población de conchas más robustas y fuertes. No obstante, los individuos de concha delgada también presentaron una tasa de mortalidad más alta. Para las dos poblaciones la oferta alimentaria fue muy baja, lo cual pudo haber influido en la baja densidad poblacional encontrada durante 2015. Es necesario resaltar la importancia del recurso ostra en la zona y la ventaja que representa para los pescadores, los especímenes de concha más robusta, los cuales pueden ser mantenidos por más tiempo sin congelar que los de concha delgada.

ABSTRACT The mangrove oyster is a species of great ecological and commercial importance in many places in the Caribbean. In Bahía de Cispatá, Colombia, the oyster represents the economic support of an important group of fishermen lacks management plans for the extraction regulation. The objective of the present study was to establish the population structure of Crassostrea rhizophorae in Bahía Cispatá, as a basic input for the future formulation of management plans. Due to the morphological differences observed among the individuals of the sampled sites, sequences of the COI gene were obtained, concluding that all individuals correspond to the species Crassostrea rhizophorae. Based on the morphological differences, two populations were differentiated, which did not show coexistence in the same sampling station. Through the FiSAT program, the main population parameters were evaluated, finding that individuals of the population with thin shells grow at a faster rate, reaching greater sizes than individuals from the population of more robust and strong shells. However, individuals with thin shells also had higher mortality rates. For both populations, the food supply was very low, which may have influenced the low population density found during 2015. It is important to highlight the importance of this resource in the area and the advantage presented by the more robust oyster's specimens for fishermen since is that it allows them to have it to be stored for longer than the thin shell oyster.

ASPECTOS MORFOLÓGICOS Y PRIMER REGISTRO DEL DINOFLAGELADO Pronoctiluca spinifera EN EL CARIBE COLOMBIANO

Se registró por primera vez la presencia del dinoflagelado atecado Pronoctiluca spinifera (Lohman) (Schiller, 1933) en el Parque Nacional Natural Corales de Profundidad-PNNCPR del Caribe colombiano. Fueron hallados a uno y 40 m de profundidad, tres ejemplares con tamaño promedio de 54,84 µm de largo y 10,29 (m de ancho; de contorno fusiforme con dos proyecciones, una en forma de tentáculo (13,56 µm) y otra puntiaguda. Los sitios de muestreo se caracterizaron por temperaturas entre 27,7 y 30,3 °C; salinidad entre 35,6 y 36,4 y clorofila a entre 0,0801 y 0,1741 mg/m3; valores típicos de ambientes oceánicos. Con el presente trabajo se amplía la distribución de P. spinifera hallándose por primera vez en el mar Caribe Colombiano en el PNNCPR.

The presence of the unarmored dinoflagellate Pronoctiluca spinifera (Lohman) Schiller (1933) was recorded for the first time in the Parque Nacional Natural Corales de Profundidad-PNNCPR, in the Colombian Caribbean. Three specimens with an average size of 54.84 µm long and 10.29 wide were found at one and 40 m depth; of the fusiform outline with two projections, one in the form of the tentacle (13.56 pm) and another pointed. The sampling sites were characterized by temperatures between 27.7 and 30.3 °C; salinity between 35.6 and 36.4 and Chlorophyll a between 0.0801 and 0.1741 mg / m3; typical values of oceanic environments. With this paper, the distribution of P. spinifera is extended, being found for the first time in the Colombian Caribbean Sea in the PNNCPR.

Dinoflagelados potencialmente tóxicos asociados a pastos marinos en Isla de Barú, Caribe Colombiano, durante El Niño 2015 / Potentially toxic dinoflagellates associated to seagrass on isla de barú, colombian caribbean, during el niño 2015

ABSTRACT In the last decades, harmful algal blooms (HAB) and toxic events such as ciguatera seem to have increased in frequency and intensity, negatively impacting human health, economy, and marine ecosystems. In Colombia, these events have caused a series of consequences ranging from the death of fish and birds to toxic effects on humans. Although some toxin-producing dinoflagellate species are common in the Caribbean, their dynamics are poorly understood, making the adoption of any regulations difficult. To determine the composition and abundance of dinoflagellates associated with seagrasses we collected 18 samples on Isla de Barú, during 2015. We found ten diatom genera and three dinoflagellate genera, Prorocentrum, Ostreopsis, and Gambierdiscus, that include toxigenic species related to ciguatera and diarrheic shellfish poisoning. Prorocentrum lima was the most abundant dinoflagellate, with average cell densities of 52±48 cells/g substrate wet weight. The temperature hypothesis gains strength as one of the main modulators of dinoflagellate abundance observed in the Caribbean, especially regarding Prorocentrum species and some diatoms such as Mastogloia corsicana and Actinocyclus normanii. This assumption is supported by the Since high positive correlation between El Niño Southern Oscillation and the sea surface temperature in the Caribbean during 2015.

RESUMEN En las últimas décadas, las floraciones de algas nocivas (FAN) y los eventos tóxicos, como la ciguatera, parecen haber aumentado en frecuencia e intensidad, afectando negativamente la salud humana, la economía y los ecosistemas marinos. En Colombia, estos eventos han causado una serie de consecuencias que van desde la muerte de peces y aves hasta efectos tóxicos en los seres humanos. Aunque algunas especies de dinoflagelados que producen toxinas son comunes en el Caribe, su dinámica es poco conocida, lo que dificulta la adopción de cualquier regulación. Para determinar la composición y abundancia de dinoflagelados asociados con los pastos marinos, recolectamos 18 muestras en Isla de Barú durante 2015. Encontramos diez géneros de diatomeas y tres géneros de dinoflagelados, Prorocentrum, Ostreopsis y Gambierdiscus, que incluyen especies toxigénicas relacionadas con la ciguatera y la intoxicación diarreica por mariscos. Prorocentrum lima fue el dinoflagelado más abundante, con densidades celulares promedio de 52 ± 48 células / g de peso húmedo de sustrato. La hipótesis de la temperatura gana fuerza como uno de los principales moduladores de la abundancia de dinoflagelados observados en el Caribe, especialmente con respecto a las especies de Prorocentrum y algunas diatomeas como Mastogloia corsicana y Actinocyclus normanii. Este supuesto está respaldado por la alta correlación positiva entre la Oscilación Sur de El Niño y la temperatura de la superficie del mar en el Caribe durante 2015.

Algal blooms detection in Colombian Caribbean Sea using MODIS imagery.

The current capacity in the Caribbean region to enhance the knowledge about algal blooms and harmful algal blooms has several logistical constraints. This work aimed to explore the detection of possible algal blooms using Moderate Resolution Imaging Spectroradiometer (MODIS) Fluorescence Line Height (FLH) data in the Colombian Caribbean Sea between 2003 and 2013. Monthly FLH images with 4 km of spatial resolution were processed and classified. The relationship between the Sea Surface Temperature (SST) and the FLH were explored using a Geographically Weighted Regression. The results showed three areas identified as having possible persistent blooms: the Urabá Gulf (UG), Magdalena Rivermouth (MRM), and Guajira Peninsula (GP). The SST does not have any considerable influence on the variation in the FLH. The supply of nutrients during the rainy season may be causing the frequent massive algae growth. MODIS fluorescence was useful as a screening tool to identify risk areas for potential algal blooms.

Ecología poblacional de Cittarium pica (Gastropoda: Trochidae) de San Andrés Isla, Reserva Internacional de Biósfera, Seaflower / Population ecology of Cittarium pica (Gastropoda: Trochidae) of San Andrés Island, International Biosphere Reserve, Seaflower

Resumen Cittarium pica es un gasterópodo conspicuo de los litorales rocosos del Caribe. Dada su importancia en la dieta de las comunidades del Caribe, éste ha sido sobrexplotado, incluso en algunos lugares se ha extinguido, por lo cual algunos países lo han incluido dentro de la lista roja de invertebrados marinos. En la isla de San Andrés Colombia la pesca es artesanal y no tiene regulaciones, conllevando a una disminución de su población. Teniendo en cuenta la importancia socioeconómica, cultural y ecológica de C. pica, así como su grado de vulnerabilidad a la extinción, es urgente desarrollar acciones de manejo que incrementen la resiliencia de la especie y el sostenimiento del recurso. Para evaluar el estado de la población de C. pica de la isla, se estimó la estructura por talla y por sexo, talla de maduración, y los parámetros de crecimiento a partir del análisis de datos de frecuencia de tallas. De septiembre a diciembre 2013 y de enero a marzo 2014, se recolectaron al azar 458 ejemplares en ocho estaciones representativas del litoral rocoso de la isla. Se midió el diámetro de la base de la concha, se determinó el sexo y el tamaño mínimo de maduración. El 80 % de la población consistió de individuos pequeños < 25.5 mm. La proporción de sexos fue de 1:1, excepto en noviembre y el tamaño mínimo de inicio de la madurez para sexos combinados fue de 14.07 mm. Los parámetros de von Bertalanffy K (0.730 año-1), L∞ (110.78 mm) y Φ (3.95) son mayores respecto a otras poblaciones del Caribe. La mortalidad total fue de 3.39 año-1, la mortalidad natural y por pesca fue de 1.02 año-1 y 2.36 año-1, respectivamente. El máximo reclutamiento se estimó para junio y la tasa de explotación máxima entre 0.563 y 0.640, para tamaños de captura de 40 y 50 mm. La tasa de explotación se estimó en 0.70, lo que sugiere una fuerte presión sobre el recurso. Por lo anterior, se recomienda vedar por completo la captura manual de C. pica hasta que un monitoreo evidencie que la población se recupera en la isla.

Abstract Cittarium pica is a conspicuous gastropod of the rocky coastlines of the Caribbean. Given its importance in the diet of Caribbean communities, it has been over-exploited, even reaching extinction in some places, and the species has been included in the red lists of marine invertebrates in some countries. Its fishery in San Andres Island-Colombia is artisanal without any regulation, leading to a decrease in its population. Taking into account the socio-economic, cultural and ecological importance of C. pica and its vulnerability to extinction, it is urgent to develop management actions directed to increase the resilience of the species and its sustainability as a fishery resource. To assess the current population state of C. pica from San Andrés Island, we estimated size structure, sex structure, maturation size, and growth parameters, based on length-frequency data analysis. From September to December 2013, and January to March 2014, we randomly collected 458 individuals at eight representative sites of the rocky shores of the island. We measured the longest diameter of the shell base, determined the sex by the color of the gonad, and thus determined the minimum maturation size. We found, that 80 % of the population consisted of small individuals < 25.5 mm sizes. The sex ratio was 1:1 except in November, where males were more abundant. The minimum size at the beginning of maturity for both sexes was 14.07 mm. The growth parameters of von Bertalanffy: K (0.730 yr-1), L∞ (110.78 mm) and Φ (3.95) were higher when compared to other populations of the Caribbean. The total mortality was 3.39 yr-1, while natural and fishing mortalities were 1.02 yr-1 and 2.36 yr-1, respectively. The maximum annual recruitment pulse was estimated for June, and the maximum exploitation rate for a sustainable yield was between 0.563 and 0.640, when the catch size was greater than 40 mm or 50 mm, respectively. The exploitation rate was estimated in 0.70, suggesting a strong extractive resource pressure. With these results we recommend the closure of the fishery and constant monitoring to promote and the population recovery is verified. Until a monitoring shows that the population recovers on the island.

Blooms of ephemrral green algae in san andres iland, international biosphere rreserve seaflower, southwestern caribbaan / Florecimientos de algas verdes efimeras en la isla de San Andrés, Reserva Internacional de Biosfera Seaflower, Caribe suroccidental

We report the presence of persistent blooms of Chaetomorpha linum in San Andres island, Southwestern Caribbean, during the year 2013.

Reportamos la presencia de florecimientos persistentes del alga verde Chaetomorpha linum en la isla de San Andrés, Caribe suroccidental.

Heterotrophic microbial activity and organic matter degradation in coastal lagoons of Colombia.

In this study we measured the community respiration and the bacterial respiration as part of the overall degradation process of organic material. Additionally, the turnover rates of the pools of dissolved free glucose and acetate as representatives of the fraction of easily degradable low molecular organic solutes were determined. The study was performed in several coastal lagoons of the "Outer Delta of the Río Magdalena" in northern Colombia. The lagoons can be separated into two groups: The first group contains highly productive brackish lagoons with chl a concentrations ranging from 62-130 micrograms/l. The second group consists of less productive freshwater lagoons with chl a between 5.5-19 micrograms/l. Turnover rates of glucose and acetate were very fast in the highly productive lagoons resulting in turnover times of less than 20 min for both compounds. In the less productive systems the cycling of glucose and acetate was much slower. Here the mean values of the turnover times were 2 hr for glucose and 1.5 hr for acetate. The rates of bacterial DNA-formation measured as thymidine incorporation differed significantly between both groups of lagoons, being very high (1.86-2.76 nmol/l/hr) in the highly productive and relatively low (0.073-0.55 nmol/l/hr) in the less productive group. Water column community respiration ranged between 122 and 16 micrograms C/l/hr with means of 88 micrograms C/l/hr in the highly and 19 micrograms C/l/hr in the less productive group. In the first group the mean values of the bacterial contribution to community respiration amounted to 37% and in the second group to 18%. The bacterial respiration was determined in an indirect way via bacterial biomass production and assuming a growth efficiency of 50%. It is discussed whether this relatively high growth efficiency allows reasonable results in both groups of lagoons.