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1.
Bioresour Technol ; 102(20): 9783-7, 2011 Oct.
Article in English | MEDLINE | ID: mdl-21865028

ABSTRACT

The thermal behavior of high-ash anthracite coal, tobacco residue and their blends during combustion processes was investigated by means of thermogravimetric analysis (20 K min(-1), ranging from ambient temperature to 1273 K). Effects of the mixed proportion between coal and tobacco residue on the combustion process, ignition and burnout characteristics were also studied. The results indicated that the combustion of tobacco residue was controlled by the emission of volatile matter; the regions were more complex for tobacco residue (four peaks) than for coal (two peaks). Also, the blends had integrative thermal profiles that reflected both tobacco residue and coal. The incorporation of tobacco residue could improve the combustion characteristics of high-ash anthracite coal, especially the ignition and burnout characteristics comparing with the separate burning of tobacco residue and coal. It was feasible to use the co-combustion of tobacco residue and high-ash anthracite coal as fuel.


Subject(s)
Coal Ash/chemistry , Nicotiana , Thermogravimetry , Hot Temperature , Temperature
2.
Biotechnol Lett ; 31(1): 163-9, 2009 Jan.
Article in English | MEDLINE | ID: mdl-18792793

ABSTRACT

A plant transformation vector, pCLKSCLA25 (EU327498), was developed to contain eight cloning sites and the inducible self-excision system which provided an effective approach to eliminate the selectable marker gene(s) from transgenic plants. Upon induction by salicylic acid, the cre gene produced a recombinase that eliminated sequences encoding the selectable marker neomycin phosphotransferase and cre itself. The excision efficiency was 41% in transgenic tomato regenarants. The stilbene synthase gene (vst1) from Vitis vinifera L. was cloned into pCLKSCLA25. The expression of vst1 gene contributed to the accumulation of trans-reveratrol from 3.4 to 8.7 mug/g fresh wt in different marker-free transgenic tomato lines.


Subject(s)
Acyltransferases/genetics , Genes, Plant , Integrases/metabolism , Recombination, Genetic/drug effects , Salicylic Acid/pharmacology , Solanum lycopersicum/enzymology , Solanum lycopersicum/genetics , Blotting, Southern , DNA, Plant/metabolism , Gene Expression Regulation, Plant/drug effects , Genetic Markers , Solanum lycopersicum/drug effects , Plant Proteins/genetics , Plant Proteins/metabolism , Plants, Genetically Modified
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