Effects of exercise on circadian rhythms in humans.

The biological clock system is an intrinsic timekeeping device that integrates internal physiology and external cues. Maintaining a healthy biological clock system is crucial for life. Disruptions to the body's internal clock can lead to disturbances in the sleep-wake cycle and abnormalities in hormone regulation, blood pressure, heart rate, and other vital processes. Long-term disturbances have been linked to the development of various common major diseases, including cardiovascular diseases, metabolic disorders, tumors, neuropsychiatric conditions, and so on. External factors, such as the diurnal rhythm of light, have a significant impact on the body's internal clock. Additionally, as an important non-photic zeitgeber, exercise can regulate the body's internal rhythms to a certain extent, making it possible to become a non-drug intervention for preventing and treating circadian rhythm disorders. This comprehensive review encompasses behavioral, physiological, and molecular perspectives to provide a deeper understanding of how exercise influences circadian rhythms and its association with related diseases.

Strain and Age Dependent Entrainable Range of Circadian Behavior in C57BL/6 and BALB/c Mice.

The mammalian circadian system has a plasticity in a certain range, rather than a strict 24-hour cycle, with considerable variations among species, strains, and ages. As the most widely used mouse strains in circadian research, C57BL/6 and BALB/c mice were well known to have different internal periods and responses to various non-24-hour light-dark cycles. However, their entrainable range of circadian behavior was not specifically studied, neither was the effect of aging. Besides, it is not well known if mice with appeared behavioral adaptation are really healthy. In the current study, we exposed C57BL/6 and BALB/c mice at 3 months and 18 months old to a series of short (T cycles < 24 h) and long (T cycles > 24 h) light-dark cycles. Wheel running activities were monitored continuously for calculation of the entrainable range and glucose homeostasis was investigated to reflect their health status. Our results showed that the range in both young and old C57BL/6 mice is between T23 and T26. By contrast, due to the strong adaptability to extreme LD cycles, the entrainable range on a circadian scale in both young and old BALB/c mice cannot be well determined. Despite the adaptation appeared at the behavioral level, glucose homeostasis revealed by glucose tolerance test and insulin tolerance test was impaired in mice upon T cycle treatment. In summary, our study explored the entrainment range in two popular mouse strains and suggested that behavioral adaptation may not well reflect their health status.

Impact of Time-Restricted Feeding to Late Night on Adaptation to a 6 h Phase Advance of the Light-Dark Cycle in Mice.

In modern society, more and more people suffer from circadian disruption, which in turn affects health. But until now, there are no widely accepted therapies for circadian disorders. Rhythmic feeding behavior is one of the most potent non-photic zeitgebers, thus it has been suggested that it was important to eat during specific periods of time (time-restricted feeding, TRF) so that feeding is aligned with environmental cues under normal light/dark conditions. Here, we challenged mice with a 6 h advanced shift, combined with various approaches to TRF, and found that food restricted to the second half of the nights after the shift facilitated adaptation. This coincided with improved resilience to sepsis. These results raise the possibility of reducing the adverse responses to jet lag by subsequent timing of food intake.