[Analysis of lumbosacral sagittal balance parameter variation in minimally invasive transforaminal lumbar interbody fusion with real-time 3D navigation techniques].

OBJECTIVE: To explore the dynamic changes of lumbosacral sagittal parameters after real-time three-dimensional navigation assisted minimally invasive transforaminal lumbar interbody fusion (MIS-TLIF) and traditional open TLIF for treatment of lumbar degenerative disease. METHODS: The clinical data of 61 patients with lumbar degenerative disease underwent single-segment surgery from September 2017 to September 2019 were retrospectively analyzed. Among them, 31 cases underwent MIS-TLIF with 3D navigation techniques (MIS-TLIF group) and another 30 cases underwent conventional open TLIF (traditional open TLIF group). The basic information, operative time and intraoperative blood loss were collected. The sagittal radiologic parameters were measured before surgery and 3 months after surgery, including lumbar lordosis (LL), segmental lordosis (SL), pelvic incidence (PI), pelvic tilt (PT), sacral slope (SS), anterior disc height (ADH), posterior disc height(PDH).And the average disc height(DH) and pelvic incidence to lumbar lordosis mismatch (PI-LL) were calculated. RESULTS: Operative time and intraoperative blood loss in MIS-TLIF group were significantly less than in traditional open TLIF group(P<0.05). In MIS-TLIF group, LL, SL, PI-LL, and DH were significantly improved at 3 months after surgery (P<0.05), while PI, PT, and SS were not statistically different from those before surgery (P>0.05). LL, PI-LL, and DH of patients in the traditional open TLIF group were significantly improved at 3 months after surgery (P<0.05), while the PI, PT, SS, and SL were not statistically different from those before surgery (P>0.05). LL change showed a significant correlation with SL change (r= 0.433, P<0.001). Change in SL closely correlated to change in ADH (r=0.621, P<0.05) and PDH(r=0.527, P<0.05). CONCLUSION: Real-time navigation-assisted MIS-TLIF and traditional open TLIF can recover DH in a short term for lumbar degenerative diseases, improve LL and PI-LL, and make the arrangement of the sagittal plane of the lumbosacral region more coordinated after surgery. But only the navigation assisted MIS -TLIF can significantly improve SL. Compared with traditional open TLIF, real-time navigation assisted MIS-TLIF in the treatment of degenerative lumbar diseases has the advantages of short operation time and less intraoperative bleeding.

[Genome-wide identification, classification and expression analyses of SET domain gene family in Arabidopsis and rice].

SET(Su(var), Enhancer of zeste (E(z)), and Trithorax) domain protein family members share the conserved SET domain. They participate in protein methylation, chromosome structure adjustment, and gene expression regulation, and play important roles in plant development. In this study, bioinformatics analysis identified 47 and 43 SET domain genes in Arabidopsis and rice, respectively. A comprehensive overview of this gene family was presented, including the gene structure, phylogeny, chromosome distribution, and conserved motifs. As a result, the SET domain genes were organized into 5 subfamilies on basis of phylogenetic relationship. Chromosome localization and gene duplication analysis showed that segmental and retrotransposition-like event may result in the SET domain gene expansion. By analyzing the developmental expression pattern of SET domain genes in Arabidopsis and rice, most of the SET domain genes were shown to be expressed in at least one tissue with the most expression in flower and pollen. Some genes showed specific expression patterns in certain tissues at certain stages, suggesting that they were closely related to tissue development. Differentially expressed genes were discovered in Arabidopsis and rice. All of the 4 differentially expressed genes in Arabidopsis were highly expressed in mature pollen. Three of the 4 differentially expressed genes in rice were highly expressed in stamen and the remaining one in young panicle.