ABSTRACT
Endometrial cancer (EC) is generally considered as a disease that affects older women. We attempt to explore the role of actinlike protein 8 (ACTL8) in EC and how it achieves its function. Based on the data from The Cancer Genome Atlas (TCGA), we found that ACTL8 expression was up-regulated in EC tissues and correlated with shorter overall survival of EC patients. ACTL8 expression was significantly associated with age, clinical-stage, or grade. Cox proportional hazards model analysis revealed that ACTL8 expression, grade, and clinical-stage were promising independent prognostic factors of EC. Knockdown of ACTL8 repressed the proliferative, migrating and invading capabilities of human EC cell lines KLE and Ishikawa. Silencing ACTL8 up-regulated the negative cell cycle regulator p21 and epithelial marker E-cadherin, and down-regulated the positive cell cycle regulator Cyclin A, mesenchymal markers MMP-9 and N-cadherin in KLE cells. Collectively, these outcomes illustrated that ACTL8 might act as a tumor facilitator during EC progression.
Subject(s)
Actins/metabolism , Biomarkers, Tumor/metabolism , Cytoskeletal Proteins/metabolism , Disease Progression , Endometrial Neoplasms/metabolism , Actins/genetics , Antigens, CD/metabolism , Biomarkers, Tumor/genetics , Cadherins/metabolism , Cell Line, Tumor , Cell Movement/genetics , Cell Proliferation/genetics , Cell Survival/genetics , Cyclin A/metabolism , Cyclin-Dependent Kinase Inhibitor p21/metabolism , Cytoskeletal Proteins/genetics , Down-Regulation/genetics , Endometrial Neoplasms/pathology , Female , Gene Knockdown Techniques , Humans , Matrix Metalloproteinase 9/metabolism , Middle Aged , Prognosis , Signal Transduction/genetics , Transfection , Up-Regulation/geneticsABSTRACT
Extrarenal Wilms' Tumors (ERWTs) are rare. There have been only 25 cases of ERWT arising from the female genital system reported in the literature. In this paper, we report a 60-year-old woman with a complaint of vaginal bleeding and a polypoid mass in the uterine cavity by sonography that was demonstrated as ERWT by pathology after resection. The pathological characteristics, histological origination, diagnosis, therapy and prognosis of ERWT in female reproductive system are discussed in this paper in the purpose of improving the diagnosis and therapy of this rare tumor.
Subject(s)
Kidney Neoplasms , Uterine Neoplasms , Wilms Tumor , Female , Humans , Kidney Neoplasms/diagnostic imaging , Kidney Neoplasms/physiopathology , Kidney Neoplasms/therapy , Male , Middle Aged , Uterine Neoplasms/diagnostic imaging , Uterine Neoplasms/physiopathology , Uterine Neoplasms/therapy , Wilms Tumor/diagnostic imaging , Wilms Tumor/physiopathology , Wilms Tumor/therapyABSTRACT
AIM: The aim of this study was to specifically investigate the clinicopathological role of expression of vascular endothelial growth factor C (VEGF-C) as well as the correlation with clinical outcomes in cervical cancer. METHOD: We carried out a comparative analysis of data from a prospective observational study of 82 patients with cervical carcinomas who underwent radical hysterectomy with bilateral adnexectomy and bilateral pelvic lymphadenectomy from January 2001 to January 2005. RESULTS: VEGF-C mRNA was identified in tumor tissues from 42 patients. In univariate analysis by Fisher's exact probability test, VEGF-C expression was significantly associated with lymph node metastasis (LNM) (pN1) (P < 0.01). In multivariate analysis by Binary logistic regression analysis, LNM was the independent relevant factor for VEGF-C mRNA expression in tumor tissues. In univariate analysis by the log-rank test, the overall 5-year survival rate of the patients with VEGF-C mRNA expression in tumor tissues was significantly lower than that of the patients without VEGF-C mRNA expression (47.6% vs. 87.5%; P < 0.01). The overall 5-year survival rate of the patients with LNM was significantly lower than that of the patients without LNM (50.0% vs 80.4%; P < 0.01). According to Cox regression multivariate analysis, VEGF-C mRNA expression in tumor tissues and LNM were independent, relevant factors for 5-year survival rate, respectively. CONCLUSIONS: Expression of VEGF-C is related to lymph node metastasis (pN1), and is a prognostic indicator for cervical cancer.
