Effects of qufeng xuanfei formula in guinea pig model of airway hyperergy.

This work aimed to clarify the potential regulating effects of Qufeng Xuanfei formula (QFXF) on airway neurogenic inflammation and its underlying target signal pathway. Guinea pig model of airway hyperergy (AHR) was used. The relative susceptibility of major proteins to airway neurogenic inflammation was assessed using Western blot immunoassay followed by being separated by SDS-PAGE. Compared to the model group, QFXF of all concentrations effectively depressed the capsaicin enhanced cough in guinea pigs and the peak values of airway resistance significantly decreased. The results illustrated that QFXF alleviated cough symptom in guinea pigs and reduced airway neurogenic inflammation when compared to AHR model group. Airway inflammation and damage, as well as the levels of NGF, SP and c-Fos in QFXF decreased the most in the high-dose group. The mechanism of antitussive activity may be associated with reducing airway inflammation. QFXF displayed effect on chronic cough through reducing the levels of neuropeptides, attenuating airway inflammation and promoting recovery from disease to decrease the airway neuro sensitivity, suggesting that the potential mechanism may be related to Ras/ERK/c-Fos pathway.

Sinomenine Attenuated Capsaicin-Induced Increase in Cough Sensitivity in Guinea Pigs by Inhibiting SOX5/TRPV1 Axis and Inflammatory Response.

BACKGROUND: Chronic cough is a common complaint which affects a large number of patients worldwide. Increased cough sensitivity is a very important cause of chronic persistent cough. However, there are limited clinical diagnosis and treatment for increased cough sensitivity. Transient receptor potential vanilloid-1 (TRPVl) is a member of the transient receptor potential (TRP) family of channels which is very closely associated with respiratory diseases. However, the mechanism through which TRPV1 that influences downstream events is still poorly understood. RESULTS: Capsaicin induced increase in cough sensitivity by upregulating the protein level of TRPV1, leading to the secretions of Substance P and neurokinin A which stimulated neurogenic inflammation. However, sinomenine, a component of traditional Chinese medicine, significantly attenuated the capsaicin-induced cough by inhibiting the expression of TRPV1 in guinea pigs. In addition, capsaicin increased the expression of SOX5 which mediated the transcriptional upregulation of TRPV1. However, pretreatment with sinomenine reduced the expression of SOX5. CONCLUSION: These results indicate that capsaicin induced increase in cough sensitivity by activating neurogenic inflammation, while sinomenine attenuated the increase in cough sensitivity by inhibiting the expressions of SOX5 and TRPV1 in guinea pigs. This finding may provide a novel target for the treatment of aggravated cough sensitivity.

Adult Attachment Style, Emotion Regulation, and Social Networking Sites Addiction.

While there is substantial evidence that emotion regulation plays a role in the maintenance of substance and behavior addiction, its role in addiction to social networking sites (SNS) remains unclear. Drawing on attachment theory, we explore whether emotion regulation mediates the relationship between insecure attachment and SNS addiction among 463 college students. The participants completed the short version of the Experience in Close Relationships Scale, Difficulties in Emotion Regulation Scale, and Chinese Social Media Addiction Scale. The results indicated that attachment anxiety positively predicted SNS addiction and that emotion regulation mediated this link. These findings suggest that individuals' affective regulation capability should be a target of future interventions and treatments.

Adult Attachment Orientations and Social Networking Site Addiction: The Mediating Effects of Online Social Support and the Fear of Missing Out.

Evidence supports predictive roles of adult attachment orientations for the maintenance of social networking site (SNS) addiction, but the underlying mechanisms are mostly unknown. Based on attachment theory, this study explored whether online social support and the fear of missing out mediated the relationship between insecure attachment and social networking site addiction among 463 college students in China. A questionnaire was used to collect data using the Experience in Close Relationship Scale-Short Form, online social support scale, fear of missing out scale, and Chinese Social Media Addiction Scale. The results showed that online social support and fear of missing out mediated the relationship between anxious attachment and social networking site addiction in parallel paths and serially, and online social support negatively mediated the relationship between avoidant attachment and social networking site addiction. Theoretically, the present study contributes to the field by showing how insecure attachment is linked to SNS addiction. Practically, these findings could aid in future studies on SNS addiction prevention and interventions. Limitations of the present study were discussed.