ABSTRACT
Five new guaiane sesquiterpenes, 1-5, were isolated from the culture broth of the endophytic fungus Xylaria sp. YM 311647, isolated from Azadirachta indica A. Juss. The structures of these compounds were elucidated on the basis of spectroscopic analyses, and their inhibitory activities against five pathogenic fungi were evaluated. All guaiane sesquiterpenes showed moderate or weak antifungal activities in a broth microdilution assay.
Subject(s)
Antifungal Agents/chemistry , Antifungal Agents/pharmacology , Azadirachta/microbiology , Sesquiterpenes, Guaiane/chemistry , Sesquiterpenes, Guaiane/pharmacology , Xylariales/chemistry , Antifungal Agents/isolation & purification , Fungi/drug effects , Humans , Mycoses/drug therapy , Sesquiterpenes, Guaiane/isolation & purificationABSTRACT
A new compound, 16-acetoxycytosporone B (1), along with four known ones, dankasterone A (2), dankasterone B (3), 3beta,5alpha,9alpha-trihydroxy-(22E,24R)-ergosta-7,22-dien-6-one (4), and cyclonerodiol oxide (5), were isolated from Phomopsis sp. YM355364, an endophytic fungus of Aconitum carmichaeli. Their structures were characterized by spectral analysis. Compound 2 exhibited significant inhibitory activity against influenza A/Thailand/Kan353/2004(H5N1) pseudovirus with all IC50 value of 3.56 microM. Compounds 1, 2, and 4 showed either moderate or weak antifungal activities against four pathogenic fungi.
Subject(s)
Antifungal Agents/isolation & purification , Ascomycota/metabolism , Antifungal Agents/chemistry , Antifungal Agents/pharmacology , Endophytes/metabolismABSTRACT
Two new butenolides, (4S)-4,10-dihydroxydodec-2-en-1,4-olide (1) and (4S)-4,8,10-trihydroxy-10-methyldodec-2-en-1,4-olide (2), together with three known compounds, MKN-003B (3), MKN-003C (4), and cyclo(Ala-Leu) (5), were isolated from the culture broth of a bacterium of the genus Streptomyces derived from soil environment. The structures of these compounds were elucidated on the basis of spectroscopic analysis. The inhibitory activities of the butenolides against eight pathogenic fungi were evaluated. All of the butenolides showed moderate-or-weak antifungal activities in a broth microdilution assay.
Subject(s)
4-Butyrolactone/analogs & derivatives , Actinobacteria/chemistry , Antifungal Agents/pharmacology , Fungi/drug effects , 4-Butyrolactone/chemistry , 4-Butyrolactone/isolation & purification , 4-Butyrolactone/pharmacology , Antifungal Agents/chemistry , Antifungal Agents/isolation & purification , Dose-Response Relationship, Drug , Microbial Sensitivity Tests , Molecular Conformation , Structure-Activity RelationshipABSTRACT
A new sesquiterpene, 10,11-dihydrocyclonerotriol (1), together with two known compounds, catenioblin C (2) and sohirnone A (3), were isolated from Trichoderma longibrachiatum YM311505, an endophytic fungus of Azadirachta indica. Their structures were characterized and identified by spectral analysis. Compounds 1-3 exhibited antifungal activities against Pyricularia oryzae and Candida albicans.
