Enhanced the treatment of ischemic stroke through intranasal temperature-sensitive hydrogels of edaravone and borneol inclusion complex.

Since ischemic stroke occurs by a combination of multiple mechanisms, therapies that modulate multiple mechanisms are required for its treatment. The combination of edaravone (EDA) and borneol can significantly ameliorate the symptoms of neurological deficits in cerebral ischemia-reperfusion model in rats. In this study, the solubility of borneol and edaravone was improved by hydroxypropyl-ß-cyclodextrin and PEG400. Furthermore, a nasal temperature-sensitive hydrogel containing both edaravone and borneol inclusion complex (EDA-BP TSGS) was developed to overcome the obstacles of ischemic stroke treatment including the obstruction of the blood-brain barrier (BBB) and the unavailability and untimely of intravenous injection. The effectiveness of the thermosensitive hydrogel was investigated in transient middle cerebral artery occlusion/reperfusion model rats (MCAO/R). The results showed that EDA-BP TSGS could significantly alleviate the symptoms of neurological deficits and decrease the cerebral infarct area and the degree of brain damage. In summary, nasal EDA-BP TSGS is a secure and effective brain-targeting formulation that may provide a viable option for the clinical prophylaxis and treatment of ischemic stroke.

Self-assembly molecularly imprinted polymers of 17ß-estradiol on the surface of magnetic nanoparticles for selective separation and detection of estrogenic hormones in feeds.

This paper reports a surface molecular self-assembly strategy for molecular imprinting on magnetic nanoparticles for selective separation and detection of estrogens in feeds. First, γ-methacryloxypropyltrimethoxysilane (MEMO) was successfully assembled at the surface magnetic nanoparticles through simple free radical polymerization, and subsequently, the copolymerization was further assembled between methacrylic acid (MAA) and ethylene glycol dimethacrylate (EGDMA) in the presence of templates 17ß-estradiol (E2). The synthesized magnetic molecularly imprinted polymers for E2 (E2-MMIPs) showed quick separation, large adsorption capacity, high selectivity and fast binding kinetics for E2. Meanwhile, a dispersive solid-phase extraction (DSPE) based on E2-MMIPs has been established for efficient separation and fast enrichment of estrogens from the feeds. The assay exhibited a linear range of 0.1-4 µM for E2 and estriol (E3) with the correlation coefficient above 0.9996 and 0.9994, respectively. Recoveries of E2 from three kinds of feeds spiked at different concentration levels ranged from 92.7% to 97.0% with RSD<4.7%, and recoveries of E3 ranged from 71.9% to 83.1% with RSD<4.9%, respectively. The method is simple and sensitive, and can be used as an alternative tool to effectively separate and enrich the trace of estrogens in agricultural products by HPLC-UV.

[Determination of constant for the inclusion complex of Yinqiao volatile oil with beta-cyclodextrin].

OBJECTIVE: To determine the constant for the inclusion complex of Yinqiao volatile oil with beta-cyclodextrin. METHOD: The stability constant for the inclusion complex of Yinqiao the volatile oil with beta-cyclodextrin was detemined under multiwavelength by ultraviolet spectroscopy, and spectroscopy feature for inclusion complex was studied. The phase solubility-curve showed the inclusion proportion. The inclusion complex of Yinqiao the volatile oil with beta-cyclodextrin was prepared by saturated aqueous solution. The DTA spectroscopy of inclusion complex was different from not only oil with beta-cyclodextrin but also the mixture of them. RESULT: The inclusion complex of Yinqiao the volatile oil with beta-cyclodextrin was formed. The experimental data of inclusion complex fitted well to the molar ratio of 1:1. There was good liner feature under multiwavelength of ultraviolet spectroscopy. CONCLUSION: Adopting multiwavelength method may determine the appearant stability constant of multicomponential traditional drug. The determination of the stability constant of the inclusion complex can be used in traditional drug.