Effects of Bacillus coagulans as an adjunct starter culture on yogurt quality and storage.

Bacillus coagulans has been widely studied for its probiotic properties. Therefore, identifying a strain that can be used as an adjunct starter culture for yogurt production would have commercial value. In this study, 30 B. coagulans strains were isolated from vegetable samples from 11 provinces or autonomous regions in China, and their pan-genomic and phylogenetic characteristics were analyzed. Phylogenetic analysis categorized 30 strains into 4 different subphylotypes, including subtype I (11 isolates), subtype II (7 isolates), subtype III (11 isolates), and subtype IV (1 isolate). Four B. coagulans strains (B. coagulans-70, B. coagulans-78, B. coagulans-79, and B. coagulans-100) were randomly selected from each subphylotype of the phylogenetic tree as adjunct starter cultures. Compared with the other tested strains, B. coagulans-70 showed the highest count in yogurt at the end of the manufacturing period. Comparative genome analysis indicated that the different bacterial levels of B. coagulans strains in yogurt may be associated with the abundance of genes related to carbohydrate transport and metabolism (e.g., sucrose utilization). Finally, differences in texture and volatile flavor compound profiles were observed between the yogurt samples. Compared with the other groups, the addition of B. coagulans-70 exerted a positive effect on the appearance and texture of yogurt products. Volatile analysis showed increased quantities of 2-heptanone, 2-nonanone, amyl alcohol, and 2-hydroxy-3-pentanone in the B. coagulans-70 group compared with control yogurts. These results above combined with the results of a sensory evaluation indicated that B. coagulans-70 is the most suitable strain for further use in functional dairy product development.

Protein diets with the role of immune and gut microbial regulation alleviate DSS-induced chronic ulcerative colitis.

The association between diet and inflammatory bowel disease (IBD) has been confirmed. However, the role of protein consumption in IBD remains controversial. This research aimed to explore the effects of milk-based protein (MBP), potato protein (PP), and mixed protein (MP) on the recovery of mice with dextran sulfate sodium (DSS)-induced ulcerative colitis (UC). MP alleviated UC symptoms through reducing colon shortening and tissue damage, decreasing neutrophil infiltration, maintaining the mucous layer integrity, and suppressing the expression of TNF-α, IL-17A, IL-6, and IL-1ß. MBP and PP decreased the colon shortening and IL-1ß levels but PP increased the MUC2 expression. Additionally, the gut microbial structure and composition were altered after different proteins supplement. Compared to DSS-treated mice, MP-treated mice showed that increased abundances in Coprococcus and Bifidobacterium and decreased abundances in Sutterella, Lactobacillus, and Akkermansia. MBP increased the proportion of Bifidobacterium and reduced Sutterella, but PP increased Ruminococcus and Bifidobacterium and decreased Adlercreutzia. Correspondence analysis of gut microbial composition to determine the effects of protein diets on immune response and pathological characteristics also verified the interaction between gut microbiota and alleviation of colitis. These results provide a theoretical basis for the selection of raw materials for clinical enteral nutrition preparations and potential use for potato protein wastes.

Electromagnetic properties of crayfish and its responses of temperature and moisture under microwave field.

To reveal the application potential of microwave heating in the thermal processing of crayfish, this work explored the electromagnetic properties of different parts of crayfish and the patterns of temperature and moisture responses in crayfish during microwave heating. The results of electromagnetic analysis demonstrated that the electromagnetic properties of different parts of crayfish were different, and the tail had higher dielectric properties and reflective loss than other parts, but the maximum thickness of each part of crayfish was almost within their heating depth of microwave. The visual imaging and numerical simulation of temperature and moisture responses showed there were nonuniform temperature and moisture distributions in crayfish during microwave heating. The crayfish tail was selectively heated and rapidly cooked, but its moisture loss was far less than the mass loss of whole crayfish. Furthermore, the immobilized water in crayfish tail meat was continuously converted to free water, while the bound water was relatively stable during microwave heating. This work provided the theoretical references for the assumption that cooking the crayfish by microwave to overcome the shortcomings of boiling. PRACTICAL APPLICATION: In this work, we innovatively applied microwave heating to the heat processing of crayfish, and analyzed the electromagnetic properties of different parts in crayfish and explored its temperature and moisture responses under microwave field. Although this is a basic research, which provided some theoretical references for the assumption that microwave heating of crayfish (Procambarus clarkia) may be a clean and efficient means of overcoming the shortcomings associated with boiling. In particular, the simulation model of crayfish was established according to its real size and shape, which provided an option for the prediction of temperature response of crayfish in the microwave field.

The physicochemical properties of chitosan prepared by microwave heating.

The aim of this study was to compare the physicochemical properties of chitosan prepared by microwave and water bath heating with an equivalent quantity of heat intake. The structure and physicochemical properties of the chitosan obtained by these two methods were characterized by Fourier transform infrared spectroscopy (FTIR), X-ray diffractometry (XRD), gel permeation chromatography (GPC), and scanning electron microscopy (SEM). The FTIR and XRD patterns show that there was no significant difference in the structure of chitosan produced by the two heat sources. The results showed that chitosan with 73.86% deacetylation was successfully prepared by microwave heating within 60 min, while a longer time of 180 min was required for the preparation of chitosan with the same deacetylation degree (74.47%) using the conventional heating method under the same heating rate. Even under the same temperature conditions, microwave technology can greatly reduce the reaction time by approximately 1/3, while the chitosan produced by microwaves can obtain relatively low molecular weight and viscosity. These results showed that microwaves may efficiently promote complete chemical reactions by the friction heating mechanism generated by molecular vibration beyond a rapid heating source, turning into a more efficient, energy-saving, and environmentally friendly method for the further use of rigid shrimp shells and highly crystalline crustacean materials.

Study on water proton distribution and flow status of starch during the hydration process.

The hydration process of starch significantly affects the quality of starch-based food, especially for samples with medium to low water content. In this paper, rice starch, as a representative of cereal starch, and potato starch were chosen as the samples. The proton distributions and flow status of the hydrated rice starch and potato starch with moisture contents of 20-90% and the causes of the differences between them were investigated by 1H LF-NMR. The longitudinal and transverse proton relaxation (T1 and T2) of the two starch systems was obtained using the inversion recovery (IR) sequence, the free induction decay (FID) sequence, and the multi-pulse echo CPMG sequence. Through the detection of longitudinal relaxation, two different populations were found in the two hydrated starch systems, and the changes were linearly related to the moisture content. Through the detection of transverse relaxation, two populations were distinguished in the hydrated rice starch samples with different moisture content, whereas two to four different populations were detected in the hydrated potato starch samples. Because of the differences in particle size and swelling capacity, hydrated potato starch showed greater proton freedom and more observable types of protons than hydrated rice starch.

¹H NMR studies of starch-water interactions during microwave heating.

The aim of the present study was to investigate the effect of microwave heating on water distribution and dynamics in starch granules during the gelatinization of starch. Starch samples treated with microwave heating, rapid conventional heating and conventional heating was measured by (1)H NMR to examine the water distribution and dynamics in rice starch granules at a water activity of 0.686. The system proton longitudinal and transverse relaxation times were determined using inversion recovery (IR) and Carr-Purcell-Meiboom-Gill (CPMG) pulse sequences. The results showed that the T1 of the water molecules in the samples treated with any of the three heating methods exhibited two distinct spectral peaks over the temperature range of 40-60 °C. With rising temperature, the long T1 component and the short T1 component approached each other, showing a trend of gradual convergence, while T2 exhibited a single peak over the entire temperature range examined. In addition, significant differences were observed in the T1 and T2 of the water molecules in the samples heated by microwave, rapid conventional and conventional. The results show that the rapid heating effect of microwave inhibits the destruction of the hydrogen bonds between starch and water molecules. In contrast, the vibration motion of polar molecules caused by microwave heating accelerates the destruction of hydrogen bonds, producing a much stronger effect than the rapid heating effect of microwave.

Structural variation of rice starch in response to temperature during microwave heating before gelatinisation.

Although it is well known that structural variations occur in starch during gelatinisation, little is known about how the structure of starch changes at subgelatinisation levels. The objective of this study was to investigate structural variations of rice starch ascribed to the temperature during microwave heating. Rapid conduction heating was used to imitate the high microwave heating rate through oil bath, which was then compared with traditional conduction heating. Structural changes due to temperature increases were investigated using thermogravimetry and differential scanning calorimetry while the distinct lamellar organisation of starch was obtained through small-angle X-ray scattering. The results showed that the structure of starch responds non-monotonically to temperature rising before gelatinisation, which was also affected by heating rates. The samples treated by microwave and rapid conduction heating essentially underwent the same thermal property changes and the molecular vibration of the microwaves did influence the submicroscopic lamellar structure.