Review of T cell proliferation regulatory factors in treatment and prognostic prediction for solid tumors.

T cell proliferation regulators (Tcprs), which are positive regulators that promote T cell function, have made great contributions to the development of therapies to improve T cell function. CAR (chimeric antigen receptor) -T cell therapy, a type of adoptive cell transfer therapy that targets tumor cells and enhances immune lethality, has led to significant progress in the treatment of hematologic tumors. However, the applications of CAR-T in solid tumor treatment remain limited. Therefore, in this review, we focus on the development of Tcprs for solid tumor therapy and prognostic prediction. We summarize potential strategies for targeting different Tcprs to enhance T cell proliferation and activation and inhibition of cancer progression, thereby improving the antitumor activity and persistence of CAR-T. In summary, we propose means of enhancing CAR-T cells by expressing different Tcprs, which may lead to the development of a new generation of cell therapies.

Using the Random Forest for Identifying Key Physicochemical Properties of Amino Acids to Discriminate Anticancer and Non-Anticancer Peptides.

Anticancer peptides (ACPs) represent a promising new therapeutic approach in cancer treatment. They can target cancer cells without affecting healthy tissues or altering normal physiological functions. Machine learning algorithms have increasingly been utilized for predicting peptide sequences with potential ACP effects. This study analyzed four benchmark datasets based on a well-established random forest (RF) algorithm. The peptide sequences were converted into 566 physicochemical features extracted from the amino acid index (AAindex) library, which were then subjected to feature selection using four methods: light gradient-boosting machine (LGBM), analysis of variance (ANOVA), chi-squared test (Chi2), and mutual information (MI). Presenting and merging the identified features using Venn diagrams, 19 key amino acid physicochemical properties were identified that can be used to predict the likelihood of a peptide sequence functioning as an ACP. The results were quantified by performance evaluation metrics to determine the accuracy of predictions. This study aims to enhance the efficiency of designing peptide sequences for cancer treatment.

Influence of smartphone use motives on smartphone addiction during the COVID-19 epidemic in China: the moderating effect of age.

With the development of science and technology, the phenomenon of smartphone addiction has become very common. However, smartphone addiction has adverse consequences. To date, few studies have examined psychological crises and smartphone use motives during the coronavirus disease 2019 (COVID-19) pandemic according to age. Therefore, this study aimed to examine the influences of different types of smartphone use motives on smartphone addiction and explore the moderating effect of age on adolescents and adults. A total of 1346 participants (600 adults and 746 adolescents) completed questionnaires on their motives for smartphone use and smartphone addiction. Results indicated significant positive correlations between smartphone use motives and smartphone addiction. In the moderation model, mood regulation, social relations, pastime, and conformity significantly and directly predicted smartphone addiction; however, perceived enjoyment did not. Age played a moderating role in the prediction of smartphone addiction. Teenagers and adults have different motives for smartphone use, and different motives have different effects on adolescents and adults. Adolescents have higher coping motivation and conformity motivation than adults, and for adolescents, perceived pleasure motivation has a significant impact on smartphone addiction. For adults, perceived pleasure and social relationship motivation have a significant impact on smartphone addiction. Therefore, interventions for smartphone addiction can be developed by investigating the motives of use among different people, and age should be considered when developing interventions for smartphone addiction.

Dominance or Integration? Influence of Sexual Dimorphism and Clothing Color on Judgments of Male and Female Targets' Attractiveness, Warmth, and Competence.

The face is an important source of information in social interactions. Prior studies exploring the mechanism of face perception were consistent with either dominance or integration theory. Studies have shown that both sexually dimorphic features and background cues play essential roles in the formation of impressions and the perception of facial attractiveness. In this study, we conducted two experiments to examine 539 participants' appraisal of attractiveness, warmth, and competence of the target faces of masculine and feminine men and women dressed in red, blue, or white. The results showed that: (1) feminized male/female faces were considered to have a higher degree of attractiveness, warmth, and competence, (2) people rated feminine faces wearing red higher in terms of attractiveness perception, while there was no significant effect of red on attractiveness perception of masculine faces, (3) when evaluating the warmth of targets, the promotion effect of red was found for feminine faces but not for masculine faces. This study, conducted in a pathogen disgust environment, provides direct evidence to support the integration theory over the dominance theory. Feminized red preference found in this study matches Chinese collectivism and the red cultural heritage, which has an important value for people's daily impression management and consumption decisions.

Non-Pregnant and Pregnant Women's Femininity Preferences in Male Faces: Tests Based on Within- and Between-Sex Sexual Dimorphism Facial Manipulations.

Past research on women's preferences for male facial masculinity in Western cultures has produced inconsistent results. Some inconsistency may be related to the use of different facial stimulus manipulations (e.g., between-sex sexual dimorphic facial manipulation or within-sex sexual dimorphic facial manipulation) that do not perfectly avoid non-facial cues, and pregnancy status may also influence women's face preferences. We therefore recruited pregnant and nonpregnant Chinese women and manipulated the sexual dimorphism of male facial stimuli to explore the influences of manipulation methods, non-facial cues, and pregnancy status on face preferences. Results showed that: (1) in contrast with a general masculinity preference observed in Western cultures, both pregnant and nonpregnant Chinese women preferred feminized and neutral male faces generally; (2) pregnant women's preference for feminized male faces was stable across manipulation methods, while nonpregnant women preferred feminized male faces except under between-sex sexual dimorphism manipulation; and (3) manipulation methods, rather than non-facial cues, influenced participants' face preferences. Specifically, women showed the strongest preferences for femininity when face stimuli were manipulated by within-sex sexual dimorphic facial manipulation, followed by unmanipulated faces and between-sex sexual dimorphic facial manipulation. This effect was stronger for nonpregnant women in the unmanipulated condition and for pregnant women in the between-sex sexual dimorphic facial manipulation. This research provides empirical evidence of women's preferences for sexual dimorphism in male faces in a non-Western culture, as well as the effects of facial manipulation methods, pregnancy status, and the interactions between these factors.

Do We See Masculine Faces as Competent and Feminine Faces as Warm? Effects of Sexual Dimorphism on Facial Perception.

Previous research on non-facial features demonstrated that masculinity and femininity correlated highly with perceived competence and warmth, respectively. Several studies focused on dimorphic facial cues and found an association between masculine faces and competence. However, there's no study exploring the association between facial dimorphism and social judgment both using explicit and implicit experimental paradigms, i.e. Triad Classification Task, Implicit Associate Task. This study examined the association of masculinity/femininity and competence/warmth via explicit and implicit measures in three experiments. The results showed that participants saw feminine/masculine faces as more consistent with warmth/competence for both male and female faces. Besides, it was found that the above associations were more obvious in female participants. The current studies extended research of effects of dimorphic facial cue in social judgment and provided direct evidence linking facial masculinity with perceived competence, and facial femininity with perceived warmth.

The (Continuous) Nature of Perceived Gender Counter-Stereotype: A Threshold Model of Gender Stereotype Maintenance.

People often expect men and women to look, act, and think like typical members of their gender groups. When men and women deviate from gender-stereotypical expectations across various domains, people tend to denigrate them, compared to those who follow stereotypical expectations. This derogatory attitude-termed the backlash effect-has been well supported by psychological research. However, previous studies on the backlash effect have often neglected the fact that men and women can be counter-stereotypical of their gender groups, to varying degrees. This research tried to address this continuous nature of counter-stereotypical characteristics in various domains using six experiments to evaluate individual responses to gendered facial cues, behaviors, and psychological traits. We conducted three studies, with two experiments per study. Most importantly, this research proposed a threshold model of gender stereotype maintenance to explain people's evaluations of gender-counter-stereotypical targets across various domains. The threshold model suggested that appraisal for a target with balanced gender-stereotypical and gender-counter-stereotypical characteristics tends to be more positive than for a target who strictly adheres to gender stereotypes or gender-counter-stereotypical characteristics. The results of all three studies supported the threshold model, which demonstrated a curvilinear pattern of participants' appraisals and targets' gender-counter-stereotypical degrees. The threshold model of stereotype maintenance has enriched the traditional stereotype maintenance theory and enlightened the development of a more effective impression management strategy. Moreover, it provided more ecological validity that treated gender counter-stereotype as a continuum rather than a binary variable.

A multi-factors rational design strategy for enhancing the thermostability of Escherichia coli AppA phytase.

Despite recent advances in our understanding of the importance of protein surface properties for protein thermostability,there are seldom studies on multi-factors rational design strategy, so a more scientific, simple and effective rational strategy is urgent for protein engineering. Here, we first attempted to use a three-factors rational design strategy combining three common structural features, protein flexibility, protein surface, and salt bridges. Escherichia coli AppA phytase was used as a model enzyme to improve its thermostability. Moreover, the structure and enzyme features of the thermostable mutants designed by our strategy were analyzed roundly. For the single mutants, two (Q206E and Y311K), in five exhibited thermostable property with a higher success rate of prediction (40 %). For the multiple mutants, the themostable sites were combined with another site, I427L, we obtained by directed evolution, Q206E/I427L, Y311K/I427L, and Q206E/Y311K/I427L, all exhibited thermostable property. The Y311K/I427L doubled thermostability (61.7 %, and was compared to 30.97 % after being heated at 80 °C for 10 min) and catalytic efficiency (4.46 was compared to 2.37) improved more than the wild-type AppA phytase almost without hampering catalytic activity. These multi-factors of rational design strategy can be applied practically as a thermostabilization strategy instead of the conventional single-factor approach.

Relationship between Escherichia coli AppA phytase's thermostability and salt bridges.

In order to study on the relationship between Escherichia coli AppA phytase's thermostability and salt bridges, and indicate an effective technical route of which factor to think about and where to modify at AppA for enhancing its thermostability, a salt bridge subtraction mutant E31Q and a salt bridge addition mutant Q307D were constructed by site-directed mutagenesis. The residual activities of the wild-type AppA phytase, E31Q and Q307D were 31.42%, 17.46%, and 40.57%, respectively, after being heated at 80°C for 10 min. The salt bridge subtraction mutant E31Q showed 13.96% thermostability decreasement, and the salt bridge addition mutant Q307D showed 9.15% thermostability enhancement than the wild-type both without the pH and temperature optimum changed. It proved salt bridges play a key role in E. coli AppA phytase's thermostability and the α/ß-domain of AppA may be sensitive to heat. Salt bridges and the α/ß-domain of AppA should have high priority to think about to enhance AppA's thermostability for commercial application. Besides, molecular dynamics simulation was used for salt bridges analysis.