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J Virol Methods ; 197: 1-6, 2014 Mar.
Article in English | MEDLINE | ID: mdl-24291739

ABSTRACT

Human papillomaviruses (HPV), particularly HPV16, are associated with most cervical cancers. Currently, although prophylactic vaccines have been developed, there is still an urgent need to develop therapeutic HPV vaccines. In this study, a novel fusion protein, HPV 16 E7-HBcAg-Hsp65 (VR111), with the goal of increasing anti-HPV16 cellular immunity was developed. VR111 was analyzed using SDS-PAGE, western-blotting, capillary isoelectric focusing (cIEF), analytical ultracentrifugation (AUC) and dynamic light scattering (DLS). Gamma interferon (IFN-γ) secretion assay was performed by enzyme-linked immunospot (ELISPOT) and ELISA to test their ability to induce cellular immune response. Significant correlation between ELISPOT and ELISA was observed (r=0.8680, p<0.0001). It was shown that VR111 could induce a significant increase in E7-specific CD8(+) T cell responses. Humoral immune response was also observed. The antibody titer levels were measured by ELISA. These results indicated that VR111 was a promising therapeutic vaccine for treatment of cervical cancer with possible therapeutic potential in clinical settings.


Subject(s)
Papillomavirus Infections/therapy , Papillomavirus Vaccines/administration & dosage , Papillomavirus Vaccines/immunology , Animals , Antibodies, Viral/blood , CD8-Positive T-Lymphocytes/immunology , Enzyme-Linked Immunosorbent Assay , Enzyme-Linked Immunospot Assay , Female , Heat-Shock Proteins/genetics , Hepatitis B Core Antigens/genetics , Interferon-gamma/metabolism , Mice , Mice, Inbred C57BL , Papillomavirus E7 Proteins/genetics , Papillomavirus E7 Proteins/immunology , Recombinant Fusion Proteins/genetics , Recombinant Fusion Proteins/immunology , Vaccines, Synthetic/administration & dosage , Vaccines, Synthetic/immunology
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