Enantio- and diastereoselective boron conjugate addition to α-alkyl α,ß-unsaturated esters.

We developed a copper-catalyzed enantio- and diastereoselective boron conjugate addition to α-alkyl α,ß-unsaturated esters under base-free conditions. The approach showed excellent enantioselectivities (87-99% ee) and moderate to good conversions (51-99%), albeit with moderate diastereoselectivities (1 : 1-17 : 1 dr). The synthetic utility of this protocol was demonstrated.

[Construction and application of economy-pollution-environment three-dimensional evaluation model for district].

For deep analysis on the regional environmental economic system, the paper analyzes the mutual relation of regional economy development, environmental quality, environmental pollution, and builds the theoretical basis. Then, the economy-pollution-environment quality three-dimensional coupling evaluation model for district is constructed. It includes economic development level index, environmental pollution index, and environmental quality index. The model is a cube, which has spatialization and visualization characteristics. The model includes 8 sub cubes, which expresses 8 types of state, e. g. low pollution-inferior quality-low level of economic development etc. The model can be used to evaluate the status of region, divide development phase, analyze evolution trend etc. It has two ways including relative meaning evaluation (RME) and absolute meaning evaluation (AME). Based on the model, Yinchuan City in the Ningxia Hui Autonomous Region is used as an example for the empirical study. Using RME, compared with Guangzhou city, The result shows that the Yinchuan City has been a high pollution-low quality-low level of economic development state for a long period during 1996-2010. After 2007, the state changed to a high pollution-high quality-low level of economic development. Now, the environmental quality of Yinchuan city gets better, but pollutant discharge pressure is high, and tends to be the break point of high environment quality and low environment. With AME, using national standard, the Yinchuan City remains a high pollution-low quality-low level of economic development state during 1996-2010. Empirical research verifies that different target reference areas and relevant national standards have different main parameters, the evaluating result has an flexible range. The dimensionless data enhances the coupling of index. The data position in model increases the visibility to the environmental management decisions. The model improves mismatches of calculated data size, time asymmetry of spatial data, verification of the former multi-target coupling model.

[Spatial and temporal characteristics of heavy metal concentration of surface soil in Hebin Industrial Park in Shizuishan northwest China].

UNLABELLED: Concentrations of Zn, Cu, Cr, Pb and Cd in 96 topsoil samples collected from the northern Hebin Industrial Park were measured by the atomic absorption detector (AAD). Spatial variability was identified by geostatistical analysis with the aid of GS + 3.0 and ArcGIS 9.0. Temporal variability of five heavy metals was contrasted to the concentration in 1985 of the Ningxia second soil census. Google earth maps in years of 2003, 2004, 2009 and 2011 have been analyzed to find the variation of the geographical distribution of factories in study Area. Result showed that mean concentrations of Zn, Cu, Cr, Pb and Cd were 100.29, 352.86, 77.56, 50.52, 4.06 mg x kg(-1), respectively, and were all higher than the background values. Using the second level of environmental quality standard for soils of China as the criterion, Zn, Cr, Pb were not risk, Cu, Cd were mild contamination and serious contamination. Spatial distribution of the concentration of the five heavy metals varies with prevailing wind direction, from the northwest to southeast, increasing gradually. Meanwhile, there were several hotspots. Google earth map analysis showed that in the northeast and southeast part appeared a large number of factories after 2009, which should be the main sources of the five heavy metals in soils. In the lowlying southwest part, although there was no factory, soils still have been polluted heavily because of another adjacent industrial area in Inner Mongolia Autonomous Region and the smoke dust was blocked by the Helan Mountain along its west. CONCLUSIONS: (1) The number of the enterprise increased and trans-boundary pollution led to the high heavy metal concentration in surface soil of study area; (2) In northwest China, because of the strong wind, smoke dusts spread very long and distribution range was extensive, and low terrain area and enterprise distribution pattern lead to the island distribution.

[Preparation and identification of OmpW monoclonal antibodies].

AIM: To prepare and characterize the mouse monoclonal antibodies against Vibrio parahaemolyticus OmpW. METHODS: The OmpW amino acid sequence from three diseased Vibrio was analyzed by Bioinformatics. Mice were immunized with r-OmpW which was highly expressed and purified in E.coli. Five Vibrio(Va, Vp, Vh, Vv, Van) were chosen as antigen for mAb selection.The characters of the anti-OmpW monoclonal antibodies were studied by Western blot, Flow Cytometry, indirect immunofluorescence. RESULTS: OmpW was testified a highly conservative membrane protein.Three clones of anti-OmpW mAb was obtained. The Ig subclass of the mAb secreted from fused cell S5C10 was IgG3, which of the titer was 4.6×10(4);. The mAb could specifically recognize Vibrio parahaemolyticus, Vibrio alginolyticus, Vibrio harveyi, Vibrio anguillarum, Vibrio vulnificus, which could not react with Pseudomonas flurosecens, Aeromonas hydrophila, Aeromonas sobria, Aeromonas hydrophila, Escherichia coli. CONCLUSION: The mAb could specially recognize five diseased Vibrio, which is a useful tool for the further study of the diagnosis of Vibrio.