ABSTRACT
Chlorophyll fluorescence techniques are used for the detection of toxic substances in samples of photosynthetic cells by measuring chlorophyll a fluorimetric parameters, which are a response of the PSII physiological status. This work was conducted to determine the effects of the herbicide bentazon (CASRN 25057-89-0) on growth and maximum quantum yield of photosystem II (Fv/Fm) in cells of the marine diatom Skeletonema costatum. Unialgal cultures were exposed to several bentazon concentrations and its effects on algal growth and Fv/Fm were determined. The traditional algal growth inhibition test (algal biomass measurements) and DCMU-induced chlorophyll a variable fluorescence measurements were determined. Our results showed that even low concentrations of bentazon rapidly lead to Fv/Fm decrease, while the effects on algal growth were detected after 24 h of exposure. The LOEC (2.81 mg L(-1)) and EC50 (13.0 mg L(-1)) determined through Fv/Fm experiments were lower than the LOEC (22.5 mg L(-1)) and EC50 (24.0 mg L(-1)) determined through algal growth inhibition experiments. This confirms that the Fv/Fm is a more sensitive parameter than algal growth for monitoring the effects of bentazon. The present results have demonstrated the applicability of Fv/Fm parameter to access the early toxicity of bentazon, as well as other PSII-inhibition compounds, before significant changes occurred in the original concentration and bioavailability of these toxicants during longer exposure times.
Subject(s)
Benzothiadiazines/toxicity , Diatoms/growth & development , Diatoms/ultrastructure , Herbicides/toxicity , Photosystem II Protein Complex/metabolism , Biomass , Chlorophyll/metabolism , Data Interpretation, Statistical , Diatoms/drug effects , Diuron/toxicity , Dose-Response Relationship, Drug , FluorescenceABSTRACT
The aim of this study was to understand how a stream ecosystem that flows from its fountainhead to its mouth inside a city, changes from a water resource to a point pollution source. A multidisciplinary descriptive approach was adopted, including the short-term temporal and spatial determination of physical, chemical, biological and ecotoxicological variables. Results showed that water quality rapidly decreases with increasing urbanization, leading the system to acquire raw sewage attributes even in the first hundred meters after the fountainheads. Despite the tidal circulation near the stream mouth being restricted by shallowness, some improvement of the water quality was detected in this area. The multidisciplinary evaluation showed to be useful for obtaining a more realistic understanding of the stream degradation process, and to forecast restoration and mitigation measures.
Este trabalho teve o objetivo de compreender como um ecossistema de córrego que flui desde as nascentes até sua desembocadura dentro de uma cidade, transforma-se de recurso hídrico em fonte pontual de poluição. Foi adotada uma abordagem descritiva multidisciplinar, incluindo a determinação espacial e temporal em escala diária de variáveis físicas, químicas, biológicas e ecotoxicológicas. Os resultados mostraram que a qualidade da água rapidamente diminui com o aumento da intensidade espacial de urbanização, levando o sistema a adquirir características típicas de esgoto bruto já após suas primeiras centenas de metros de curso. Apesar da circulação relacionada à maré junto a desembocadura ser restrita devido às baixas profundidades, foi registrada certa melhora na qualidade da água nessa área. A avaliação multidisciplinar se mostrou útil para obter uma compreensão mais realista do processo de degradação do córrego e para propor medidas de restauração ou mitigação dos impactos.
Subject(s)
Environmental Monitoring , Water Movements , Water Supply , Water Pollution/analysis , BrazilABSTRACT
The aim of this study was to understand how a stream ecosystem that flows from its fountainhead to its mouth inside a city, changes from a water resource to a point pollution source. A multidisciplinary descriptive approach was adopted, including the short-term temporal and spatial determination of physical, chemical, biological and ecotoxicological variables. Results showed that water quality rapidly decreases with increasing urbanization, leading the system to acquire raw sewage attributes even in the first hundred meters after the fountainheads. Despite the tidal circulation near the stream mouth being restricted by shallowness, some improvement of the water quality was detected in this area. The multidisciplinary evaluation showed to be useful for obtaining a more realistic understanding of the stream degradation process, and to forecast restoration and mitigation measures.
