ABSTRACT
This work aimed to evaluate the quantitative parameters of fresh and thawed sperm of Canindé goats in theState of Piauí. Ten sperm samples were collected, using artificial vagina, from four goats at reproductive age. Eachejaculate was evaluated for volume, sperm concentration, mass motility, total and progressive motility, percentage ofmotile spermatozoa and vigor, for further dilution in ACP-101. Then, the sperm was filled in 0.25 mL straws,cryopreserved using the TK3000 machine and stored in nitrogen cylinders at -196 °C, for further analysis at SCA. Thesperm parameters were submitted to the T test for comparison between the fresh and thawed groups. The values forfresh sperm relative to total and progressive motility, vigor and sperm viability were higher (P < 0.05) whencompared to thawed, although both fresh and thawed sperm of these animals could be used in programs of artificial insemination (IA).
Subject(s)
Male , Animals , Cryopreservation/methods , Cryopreservation/veterinary , Ruminants/embryology , Ruminants/physiologyABSTRACT
The objective of this study was to evaluate the oestrus and pregnancy using seven and 14 dayssynchronization protocol followed by fixed-time artificial insemination (FTAI) in sheep, 50 ewes were selectedand divided into two groups: G-14 (n = 25) and G-07 (n = 25). Were inserted vaginal sponges that remained forseven and 14 days, time when were applied e.G.C. and Cloroprostenol, this only in the G-07. Oestrus and FTAIwere 55 hours after. It was used sperm diluted in ACP-102 with 5% egg yolk, and refrigerated at 4 °C for fourhours. The results were: oestrus rates of 60% and 84%; pregnancy rates of 56% and 72%; lambing of 48% and68%, for G-07 and G- 14, respectively (P > 0.05). In conclusion, the short protocol was effective insynchronizing estrus wooless sheep, as well as long-term protocol.
Subject(s)
Female , Animals , Insemination, Artificial/methods , Insemination, Artificial/veterinary , Sheep/embryology , Estrus Synchronization/methods , OvulationABSTRACT
The objective of this study was to evaluate the oestrus and pregnancy using seven and 14 dayssynchronization protocol followed by fixed-time artificial insemination (FTAI) in sheep, 50 ewes were selectedand divided into two groups: G-14 (n = 25) and G-07 (n = 25). Were inserted vaginal sponges that remained forseven and 14 days, time when were applied e.G.C. and Cloroprostenol, this only in the G-07. Oestrus and FTAIwere 55 hours after. It was used sperm diluted in ACP-102 with 5% egg yolk, and refrigerated at 4 °C for fourhours. The results were: oestrus rates of 60% and 84%; pregnancy rates of 56% and 72%; lambing of 48% and68%, for G-07 and G- 14, respectively (P > 0.05). In conclusion, the short protocol was effective insynchronizing estrus wooless sheep, as well as long-term protocol.(AU)
Subject(s)
Animals , Female , Sheep/embryology , Estrus Synchronization/methods , Insemination, Artificial/methods , Insemination, Artificial/veterinary , OvulationABSTRACT
This work aimed to evaluate the quantitative parameters of fresh and thawed sperm of Canindé goats in theState of Piauí. Ten sperm samples were collected, using artificial vagina, from four goats at reproductive age. Eachejaculate was evaluated for volume, sperm concentration, mass motility, total and progressive motility, percentage ofmotile spermatozoa and vigor, for further dilution in ACP-101. Then, the sperm was filled in 0.25 mL straws,cryopreserved using the TK3000 machine and stored in nitrogen cylinders at -196 °C, for further analysis at SCA. Thesperm parameters were submitted to the T test for comparison between the fresh and thawed groups. The values forfresh sperm relative to total and progressive motility, vigor and sperm viability were higher (P < 0.05) whencompared to thawed, although both fresh and thawed sperm of these animals could be used in programs of artificial insemination (IA).(AU)