Evaluation of Cameriere and Willems age estimation methods in panoramic radiographs of Brazilian children.

The importance of age estimation in the forensic field is inherent to the process of establishing the biological profile of children, sub-adults and adults. The established profile might be useful for the identification of deceased victims or living individuals when it comes to age of legal interest. In parallel, age estimation is also investigated for clinical purposes, especially for the diagnosis of dental and bone maturation. Several studies were developed to provide accurate age estimation methods based on skeletal and dental development. This study aimed to apply and compare Cameriere's and Willems' methods for dental age estimation in a Brazilian sample. Two examiners performed image analysis and method application in 180 panoramic radiographs of Brazilian children aged 6-14 years old. The ages estimated with both methods revealed a good correlation with the chronological ages of Brazilian boys and girls. Cameriere's method showed a slight underestimation of 0.05 years for girls and 0.03 for boys. Willems' method, on the other hand, showed an overestimation of -0.47 years for girls and -0.39 for boys. Better age estimates were obtained combining the outcomes of both methods. In practice, Cameriere's and Willems' methods reached reliable outcomes and could be applied for dental age estimation purposes.

Morphologic alterations ear, nose and lip detected with aging through facial photoanthropometric analysis.

BACKGROUND: Understanding the morphologic alterations of the human face over the time is an essential step towards optimal simulations of facial age progression. In practice, these simulations contribute to the forensic routine by tracking and recognizing missing persons. OBJECTIVE: This study aimed to assess the morphometric facial alterations with aging - concerning specifically the development of ear, nose and lip in relation to other facial structures. MATERIAL AND METHODS: The sample consisted of 700 photographs taken standardly in frontal view from male and female Caucasian subjects aged from 20 to 80 years old. The sample was divided in 7 age groups (20, 30, 40, 50, 60, 70 and 80 years old), in which 50 males and 50 females were distributed homogenously. Photoanthropometric analysis was applied with SAFF 2D® 2.0.05 (SEPAEL/INC, Federal Police, Brazil) software package considering 36 facial landmarks. A single examiner analyzed the sample aided by two other examiners for the assessment of intra-/inter-rater reliability. RESULTS: The most evident alterations found in the facial structures consisted of the enlargement of the nose and ears with aging, as well the reduction in size of the lip thickness. These alterations varied considerably in the specific age ranges studied in this research being different between males and females. CONCLUSION: The present outcomes indicated the possibility of quantifying morphometric alterations observed in the human face with aging. These alterations may support the forensic practice providing tools for age estimation based on photographic analyses.

The Brazilian identified human osteological collections.

This short communication aims to inform the scientific community of the existence of seven identified osteological collections in Brazil, most of which are housed in universities: 21st Century Collection of the Center for Studies in Forensic Anthropology, Recife; Identified Skeletal Collection of Sergipanos of the University Tiradentes, Aracaju; Identified Skull Collection of the State of Bahia, Aracaju; Osteological and Tomographic Collection - Prof. Dr. Eduardo Daruge, Piracicaba; Osteological Collection of the Institute of Teaching and Research in Forensic Sciences, Guarulhos; Identified Skull Collection of the Anatomy Museum Alfonso Bovero, São Paulo; and the Identified Skull Collection, São Paulo. Three of these collections are from a northeast population, whereas the others are from individuals from the southeast region. Altogether, there are 925 skeletons and more than 998 skulls (three of the referenced collections have only skulls). Data on the number of individuals, sex, age, origin, and dates of inhumation and exhumation are available. This large quantity of identified skeletal remains is a fundamental source of research material that can be used to characterize the Brazilian population and facilitate the development of forensic anthropology. When contextualized within the reference series from South and Central America, it is clear that the Brazilian skeletal reference series hold a position of prominence.

Evaluation of the Implementation of Good Handling Practices in Food and Beverage Areas of Hotels.

Because of the major international-level events that have recently been held in Brazil, concerns about the sensory and hygienic-sanitary conditions of food have increased. The objective of this study was to evaluate the implementation of good handling practices in food and beverage areas of hotels, with and without outsourced professional intervention. We evaluated 19 food and beverage areas in hotels in Porto Alegre, Rio do Sul, Brazil, using a checklist that was developed by a municipal surveillance team based on existing laws for good handling practices. The evaluation was done by a skilled professional in the food safety area on two occasions, at the beginning of the study (January to May 2013) and at the end (July to November 2014), and the establishments were classified as good, regular, or poor. After the baseline evaluation, an action plan listing the noncompliance found at each location was given to those responsible for the establishments, and a period of 1 year 6 months was stipulated for improvements to be made. In the repeat evaluation, those responsible for the establishments were asked whether they had hired an outsourced professional to assist them in the improvements. The hotels showed improvement during the repeat evaluation, but a significant increase in the percentage of overall adequacy was seen only in the food and beverages areas of the 12 hotels that used the intervention of an outsourced professional. The better percentage of adequacy in establishments with outsourced professional intervention underlines the importance of an external and impartial view of routine activities in the implementation of good handling practices.

Safety of pamidronate in patients with renal failure and hypercalcemia.

Pamidronate (APD) is a drug widely used for the treatment of hypercalcemia of malignancy. Renal impairment has been associated with the use of other bisphosphonates in humans, and nephrotoxicity has been described after APD administration in animals. We retrospectively evaluated the safety and efficacy of APD administration in 31 patients with underlying renal insufficiency who received 33 courses of APD in doses of 60-90 mg. Hypercalcemia resolved or improved in 91% of the patients and only 1 case had severe hypocalcemia. A transient deterioration in renal function was observed in 8 courses but this was unrelated to APD administration. No systemic ill effects were observed. APD appears to be a safe drug in patients with underlying renal failure.

Estudo da via intramedular como protocolo alternativo para a infusäo de fluidos em cäes jovens / Clinical study of intramedullary route as an alternative for fluid administration in young dogs

O uso da via intramedular foi avaliado em 31 cäes clinicamente normais, de até três meses de idade e com peso entre 920 e 3.385 g, divididos, aleatoriamente, em grupo 1 (G1), com 15 animais, e grupo 2 (G2), com 16. Aqueles do G1 foram tratados com NaCl a 0,9 por cento e os do G2 com sangue total. Foram anotados os tempos gastos para implantaçäo da agulha, as velocidades máximas de infusäo obtidas e as ocorrências havidas durante o período de infusäo. Todos os animais foram avaliados clinicamente durante os 30 dias de experimentaçäo e por meio de radiografias nos dias 15 e 30 do experimento. Constatou-se que a técnica é de fácil e rápida execuçäo e pouco cruenta. Os resultados observados indicam que a via intramedular é segura, sendo uma alternativa valiosa à via intravenosa no tratamento de filhotes de cäes com graves alteraçöes hemodinâmicas