ABSTRACT
BACKGROUND:: Heparanase is an enzyme that cleaves heparan sulfate chains. Oligosaccharides generated by heparanase induce tumor progression. Basal cell carcinoma and squamous cell carcinoma comprise types of nonmelanoma skin cancer. OBJECTIVES:: Evaluate the glycosaminoglycans profile and expression of heparanase in two human cell lines established in culture, immortalized skin keratinocyte (HaCaT) and squamous cell carcinoma (A431) and also investigate the expression of heparanase in basal cell carcinoma, squamous cell carcinoma and eyelid skin of individuals not affected by the disease (control). METHODS:: Glycosaminoglycans were quantified by electrophoresis and indirect ELISA method. The heparanase expression was analyzed by quantitative RT-PCR (qRTPCR). RESULTS:: The A431 strain showed significant increase in the sulfated glycosaminoglycans, increased heparanase expression and decreased hyaluronic acid, comparing to the HaCaT lineage. The mRNA expression of heparanase was significantly higher in Basal cell carcinoma and squamous cell carcinoma compared with control skin samples. It was also observed increased heparanase expression in squamous cell carcinoma compared to the Basal cell carcinoma. CONCLUSION:: The glycosaminoglycans profile, as well as heparanase expression are different between HaCaT and A431 cell lines. The increased expression of heparanase in Basal cell carcinoma and squamous cell carcinoma suggests that this enzyme could be a marker for the diagnosis of such types of non-melanoma cancers, and may be useful as a target molecule for future alternative treatment.
Subject(s)
Carcinoma, Basal Cell/enzymology , Carcinoma, Squamous Cell/enzymology , Glucuronidase/metabolism , Glycosaminoglycans/metabolism , Skin Neoplasms/enzymology , Eyelids/enzymology , Glucuronidase/genetics , Glycosaminoglycans/analysis , Humans , Hyaluronic Acid/analysis , Hyaluronic Acid/metabolism , Keratinocytes/metabolism , RNA, Messenger/metabolism , Real-Time Polymerase Chain Reaction/methodsABSTRACT
BACKGROUND:: Chronic urticaria is characterized by transient, pruritic lesions of varying sizes, with central pallor and well-defined edges, with disease duration longer than six weeks. Its cellular infiltrate consists of neutrophils, lymphocytes and eosinophils. There is a subgroup of patients with eosinophilic or neutrophilic urticaria, resistant to the treatment with antihistamines, but that respond to a combination of antihistamine with other drugs. OBJECTIVE:: To evaluate the present infiltration in chronic urticaria biopsies and correlate it with the clinical disease activity and response to treatment. METHODS:: Forty-one patients with chronic urticaria were classified according to the score of severity of the disease, response to treatment and type of perivascular infiltrate. Inflammatory infiltrates were divided in eosinophilic (46.30%), neutrophilic and mixed. RESULTS:: An association was found between the eosinophilic infiltrate and clinical scores of greater severity (p = 0.002). CONCLUSION:: This association shows that the eosinophilic inflammatory infiltrates denote high clinical activity, which means more severe and exuberant clinical pictures of the disease.
Subject(s)
Eosinophils/pathology , Neutrophil Infiltration/physiology , Urticaria/pathology , Urticaria/physiopathology , Adult , Biopsy , C-Reactive Protein/analysis , Chronic Disease , Cross-Sectional Studies , Female , Humans , Immunoglobulin E/analysis , Male , Reference Values , Severity of Illness Index , Statistics, Nonparametric , Treatment Outcome , Urticaria/therapyABSTRACT
O Carcinoma metastático inflamatório da mama é uma entidade incomum mas bem conhecida. Relatamos um caso em paciente do sexo feminino, previamente submetida a mastectomia bilateral por adenocarcinoma. O aspecto clínico da pele infiltrada pela neoplasia, pode simular um processo inflamatório ou infeccioso e dar margem à confusao diagnóstica com o eritema observado em consequência a outras patologias como erisipela ou celulite.
