ABSTRACT
Introduction: Chronic kidney disease promotes changes in the zinc nutritional status and in the antioxidant defense system. This study assessed the relationship between the parameters of the zinc nutritional status and the activity of superoxide dismutase in patients with chronic renal failure who are receiving hemodialysis. Methods: 134 individuals, aged between 18 and 85years, were divided into two groups: case group (hemodialyzed patients, n = 63) and control group (n = 71). Zinc concentrations in plasma and erythrocytes were determined using the flame atomic absorption spectrophotometry technique. The activity of superoxide dismutase enzyme was determined according to Ransod kit. Results: The mean values of plasma zinc were 62.02 ±13.59 μg/dL and 65.58 ± 8.88 μg/dL, and for erythrocytary zinc the values were 54.52 ± 22.82 μgZn/gHb and48.01 ± 15.08 μgZn/gHb for the chronic renal patients andt he control group, respectively. The activity of superoxidedismutase was significantly lower in patients when compared with the control group (p < 0.05). Conclusion: The activity of superoxide dismutase in patients with chronic renal failure undergoing hemodialysis, which is influenced by zinc concentracions, was significantly lower. There was an inadequate response o this enzyme to oxidative stress in patients undergoing hemodialysis (AU)
Introducción: La enfermedad renal crónica produce cambios en el estado nutricional del zinc y en el sistema de defensa antioxidante. Por lo tanto, este estudio investigó la relación entre parámetros del estado nutricional del zinc y la actividad de la enzima superóxido dismutase en pacientes con enfermedad renal crónica en hemodiálisis. Métodos: Se incluyeron 134 personas, de 20 a 59 años de edad que fueron divididos en dos grupos: grupo caso(pacientes en hemodiálisis, n = 63) y grupo control (n =71). El zinc plasmático y eritrocitario fueron analizados según el método de espectrofotometría de absorción ató-mica. La actividad de la enzima superóxido dismutasa fue analizada de acuerdo con Kit Ransod. Resultados: Los valores medios de zinc plasmático fueron 62,02 ± 13,59 μg/dL y 65,58 ± 8,88 μg/dL y los valores de zinc eritrocitário fueron 54,52 ± 22,82 μgZn/gHb y48,01 ± 15,08 μgZn/gHb, en los pacientes en hemodiálisisy en el grupo control, respectivamente. La actividad de la superóxido dismutasa fue significantemente inferior en los pacientes que en controles (p < 0,05). Conclusiones: La actividad de la superóxido dismutasa en pacientes con enfermedad renal crónica en hemodiálisis, que es influenciada por la concentración del zinc, fue significantemente inferior. Hubo una respuesta inadecuada por la enzima al estrés oxidativo en pacientes en hemodiálisis (AU)
Subject(s)
Humans , Male , Female , Young Adult , Adult , Middle Aged , Renal Dialysis , Renal Insufficiency, Chronic/complications , Zinc/analysis , Nutrition Disorders/epidemiology , Superoxide Dismutase/biosynthesis , Oxidative StressABSTRACT
INTRODUCTION: Chronic kidney disease promotes changes in the zinc nutritional status and in the antioxidant defense system. This study assessed the relationship between the parameters of the zinc nutritional status and the activity of superoxide dismutase in patients with chronic renal failure who are receiving hemodialysis. METHODS: 134 individuals, aged between 18 and 85 years, were divided into two groups: case group (hemodialyzed patients, n = 63) and control group (n = 71). Zinc concentrations in plasma and erythrocytes were determined using the flame atomic absorption spectrophotometry technique. The activity of superoxide dismutase enzyme was determined according to Ransod kit. RESULTS: The mean values of plasma zinc were 62.02 ± 13.59 µg/dL and 65.58 ± 8.88 µg/dL, and for erythrocytary zinc the values were 54.52 ± 22.82 µgZn/gHb and 48.01 ± 15.08 µgZn/gHb for the chronic renal patients and the control group, respectively. The activity of superoxide dismutase was significantly lower in patients when compared with the control group (p < 0.05). CONCLUSION: The activity of superoxide dismutase in patients with chronic renal failure undergoing hemodialysis, which is influenced by zinc concentracions, was significantly lower. There was an inadequate response of this enzyme to oxidative stress in patients undergoing hemodialysis.
