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1.
Ophthalmic Genet ; 37(4): 369-376, 2016 12.
Article in English | MEDLINE | ID: mdl-26915021

ABSTRACT

BACKGROUND: Dense deposit disease and atypical hemolytic uremic syndrome are often caused by Complement Factor H (CFH) mutations. This study describes the retinal abnormalities in dense deposit disease and, for the first time, atypical haemolytic uremic syndrome. It also reviews our understanding of drusen pathogenesis and their relevance for glomerular disease. METHODS: Six individuals with dense deposit disease and one with atypical haemolytic uremic syndrome were studied from 2 to 40 years after presentation. Five had renal transplants. All four who had genetic testing had CFH mutations. Individuals underwent ophthalmological review and retinal photography, and in some cases, optical coherence tomography, and further tests of retinal function. RESULTS: All subjects with dense deposit disease had impaired night vision and retinal drusen or whitish-yellow deposits. Retinal atrophy, pigmentation, and hemorrhage were common. In late disease, peripheral vision was restricted, central vision was distorted, and there were scotoma from sub-retinal choroidal neovascular membranes and atypical serous retinopathy. Drusen were present but less prominent in the young person with atypical uremic syndrome due to a heterozygous CFH mutation. CONCLUSIONS: Drusen are common in forms of C3 glomerulopathy caused by compound heterozygous or heterozygous CFH mutations. They are useful diagnostically but also impair vision. Drusen have an identical composition to glomerular deposits. They are also identical to the drusen of age-related macular degeneration, and may respond to the same treatments. Individuals with a C3 glomerulopathy should be assessed ophthalmologically at diagnosis, and monitored regularly for vision-threatening complications.


Subject(s)
Complement C3/immunology , Glomerulonephritis, Membranoproliferative/diagnosis , Retinal Drusen/diagnosis , Vision Disorders/diagnosis , Adult , Aged, 80 and over , Atypical Hemolytic Uremic Syndrome/diagnosis , Atypical Hemolytic Uremic Syndrome/genetics , Complement Factor H/genetics , Complement Pathway, Alternative/genetics , Electrooculography , Electroretinography , Female , Fluorescein Angiography , Glomerulonephritis, Membranoproliferative/genetics , Glomerulonephritis, Membranoproliferative/immunology , Humans , Male , Middle Aged , Retinal Drusen/genetics , Risk Factors , Tomography, Optical Coherence , Vision Disorders/genetics
2.
Inorg Chem ; 41(22): 5699-705, 2002 Nov 04.
Article in English | MEDLINE | ID: mdl-12401074

ABSTRACT

The new compound trifluoroacetylsulfenyl trifluoroacetate, CF(3)C(O)SOC(O)CF(3), which possesses two identical carbonyl substituents attached to the S-O bond, has been synthesized. The IR and UV spectra of the gas phase as well as the (13)C NMR spectrum of the solution in CDCl(3) were recorded and assigned. Quantum chemical calculations were performed with the ab initio methods HF and MP2 and the density functional approach B3LYP. The 6-31G basis set was chosen in all calculations. The molecule possesses a skew structure, and according to all computational methods, the syn-syn structure (C=O bonds of both C(O)CF(3) groups synperiplanar to S-O bond) represents the most stable conformer. In agreement with the quantum chemical calculations, the presence of small amounts (< or =5%) of a second conformer (anti-syn) cannot be excluded on the basis of the IR spectrum. The calculated values for the torsional angle around the S-O bond (delta(C-S-O-C)) of the syn-syn form are smaller than 80 degrees (72-78 degrees). Comparison with theoretical results for the corresponding disulfide CF(3)C(O)SSC(O)CF(3) and peroxide CF(3)C(O)OOC(O)CF(3) indicates that the structural properties of sulfenyl compounds are more similar to those of disulfides than to those of peroxides.

3.
Inorg Chem ; 40(7): 1672-6, 2001 Mar 26.
Article in English | MEDLINE | ID: mdl-11261978

ABSTRACT

The molecular structures and conformational properties of acetyl peroxynitrate (PAN, CH3C(O)OONO2) and trifluoroacetyl peroxynitrate (FPAN, CF3C(O)OONO2) were investigated in the gas phase by electron diffraction (GED), microwave spectroscopy (MW), and quantum chemical methods (HF/3-21G, HF/6-31G*, MP2/6-31G*, B3PW91/6-31G*, and B3PW91/6-311+G*). All experimental and theoretical methods show the syn conformer (C=O bond of acetyl group syn to O-O bond) to be strongly predominant relative to the anti conformer. The O-NO2 bonds are extremely long, 1.492(7) A in PAN and 1.526(10) A in FPAN, which correlates with their low bond energy and the easy formation of CX3C(O)OO* and *NO2 radicals in the atmosphere. The O-O bonds (1.418(12) A in PAN and 1.408(8) A in FPAN) are shorter than that in hydrogen peroxide (1.464 A). In both compounds the C-O-O-N dihedral angle is close to 85 degrees.

4.
Spectrochim Acta A Mol Biomol Spectrosc ; 56A(8): 1533-41, 2000 Jul.
Article in English | MEDLINE | ID: mdl-10907882

ABSTRACT

The conformational and tautomeric compositions of malonamide, NH2-C(O)-CH2-C(O)-NH2 were determined by vibrational spectroscopy and theoretical calculations (HF/6-31G*, B3PW91/6-31G*). Solid state Fourier transform infrared and Raman spectra were analysed. They reveal the existence of a diketo tautomer. Theoretical calculations predict a diketo structure belonging to the C1 symmetry group. No enol form is present in the molecule in the solid. 13C-NMR studies show only signals of a diketo tautomer.


Subject(s)
Malonates/chemistry , Magnetic Resonance Spectroscopy/methods , Spectroscopy, Fourier Transform Infrared/methods , Spectrum Analysis, Raman/methods
5.
Gene ; 185(1): 11-7, 1997 Jan 31.
Article in English | MEDLINE | ID: mdl-9034307

ABSTRACT

Whilst searching for a mammalian homologue of the Drosophila glass gene we cloned a mouse cDNA whose deduced sequence encodes a 614 amino acid (aa) protein with ten Cys2-His2 (C2H2) zinc finger (Zf) motifs. Zfp64 is expressed in all developing and mature mouse tissues examined, except the mouse erythroleukemia (MEL) cell line. Zfp64 maps to the distal region of mouse chromosome 2 close to lens opacity 4 (Lop4), a semidominant cataract mutation. Sequence analysis shows that Zfp64 has multiple potential phosphorylation sites for casein kinase II (CK II), protein kinase C (PKC), tyrosine kinase (TK) and c-AMP- and c-GMP-dependent protein kinase (cA/GMPDPK).


Subject(s)
Chromosome Mapping , DNA-Binding Proteins/genetics , Drosophila Proteins , Nuclear Proteins , Transcription Factors , Zinc Fingers/genetics , Amino Acid Sequence , Animals , Base Sequence , Blotting, Northern , Blotting, Southern , Casein Kinase II , Cloning, Molecular , Culture Techniques , Cyclic AMP-Dependent Protein Kinases/metabolism , Cyclic GMP-Dependent Protein Kinases/metabolism , DNA, Complementary/genetics , DNA-Binding Proteins/metabolism , Drosophila/genetics , Eye/growth & development , Eye/metabolism , Gene Expression Regulation , Gene Expression Regulation, Developmental , Gene Library , Head/growth & development , Mice , Molecular Sequence Data , Phosphorylation , Photoreceptor Cells, Invertebrate , Protein Kinase C/metabolism , Protein Serine-Threonine Kinases/metabolism , Protein-Tyrosine Kinases/metabolism , Ribonucleases/metabolism
6.
Int Ophthalmol Clin ; 37(4): 251-60, 1997.
Article in English | MEDLINE | ID: mdl-9429945

ABSTRACT

Isolated metastases to the retina or optic nerve are extremely rare. Retinal metastases arise from cutaneous malignant melanoma and carcinomas (lung, gastrointestinal, genitourinary, and breast). Most patients present with reduced vision. The clinical appearance of these metastases is variable. Melanoma metastases are brown or black, whereas carcinoma metastases are white. Frequently, retinal hemorrhages and exudates are seen. Optic nerve metastases arise from carcinomas (lung, breast, and gastrointestinal) in adults and acute leukemias in children. As is true of retinal metastases, most patients present with reduced vision. Though in most cases there is a visible optic nerve mass, some patients have optic disc edema only. Investigations are directed by a known history of malignancy in many patients and by physical examination. Cytological workup of vitreous may be required. Treatment options include observation, radiotherapy in eyes with visual potential, and enucleation for pain control. The visual prognosis is poor. In patients with retinal or optic nerve metastases, median survival is approximately 9 months after the onset of visual symptoms, but is slightly longer in cases in which the primary lesion is breast carcinoma.


Subject(s)
Neoplasms/pathology , Optic Nerve Neoplasms/secondary , Retinal Neoplasms/secondary , Adult , Aged , Combined Modality Therapy , Diagnosis, Differential , Female , Humans , Male , Middle Aged , Optic Nerve Neoplasms/pathology , Optic Nerve Neoplasms/therapy , Prognosis , Retinal Neoplasms/pathology , Retinal Neoplasms/therapy
7.
J Clin Neuroophthalmol ; 11(4): 221-7, 1991 Dec.
Article in English | MEDLINE | ID: mdl-1838538

ABSTRACT

The fundus fluorescein angiograms of 13 patients with visual disturbance due to biopsy-proven giant cell arteritis (11 with anterior ischemic optic neuropathy (AION); 2 with visual obscurations only) were compared with the fluorescein angiograms from 33 patients with acute nonarteritic AION and 23 age-matched normal eyes. In all 13 patients with giant cell arteritis, the fluorescein angiograms showed a significant delay of choroidal filling time (mean 69 seconds) in comparison with either normal subjects (mean 5.8 seconds) or patients with nonarteritic AION (mean 5.5 seconds). In patients presenting with acute AION, the finding of delayed choroidal filling on fluorescein angiography should raise the index of suspicion of giant cell arteritis and lead to prompt investigation and treatment.


Subject(s)
Choroid/blood supply , Giant Cell Arteritis/physiopathology , Ischemia/physiopathology , Optic Nerve Diseases/physiopathology , Acute Disease , Adult , Aged , Aged, 80 and over , Female , Fluorescein Angiography , Follow-Up Studies , Fundus Oculi , Giant Cell Arteritis/diagnosis , Humans , Ischemia/diagnosis , Male , Optic Nerve Diseases/diagnosis , Perfusion , Visual Acuity
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