Description of rare echinostomatid fluke Echinodollfusia longiuscula n. sp. (Trematoda: Echinostomatidae) parasitizing white stork with an amended diagnosis of the genus Echinodollfusia.

A new species of digenean, Echinodollfusia longiuscula n. sp. (Trematoda: Echinostomatidae), is described and illustrated based on specimens collected from the intestine of the white stork Ciconia ciconia (L.) (Aves: Ciconiidae) from eastern Slovakia and dissected soon after its spring arrival from African wintering. The new species differs from the two existing congeners Echinodollfusia stenon (Dollfus, 1950) and Echinodollfusia bulgarica Vassilev (Sofia 6:327-338, 1958) clearly by the oesophagus bearing diverticula, the bipartite seminal vesicle and gonads situated entirely in anterior body half. The new species E. longiuscula n. sp. can also be distinguished by possessing the following combination of features: a slender body of 12.85-21.06-mm long and maximum of 581-855-µm wide; head collar with 43-47 small collar spines, dorsal in double row; oral sucker more than half the size as ventral sucker, small pharynx, oval and in tandem testes situated pre-equatorial, small ovary at the boundary of anterior third of the body and anteriormost extent of the vitelline fields just reaching the level of posterior margin of the ventral sucker. An amended generic diagnosis of little-known Echinodollfusia Skrjabin et Bashkirova (Osnovy Trematodologii 12:51-930, 1956) is also given here.

Angiogenic gene therapy in patients with nonrevascularizable ischemic heart disease: a phase 2 randomized, controlled trial of AdVEGF(121) (AdVEGF121) versus maximum medical treatment.

The demonstration that angiogenic growth factors can stimulate new blood vessel growth and restore perfusion in animal models of myocardial ischemia has led to the development of strategies designed for the local production of angiogenic growth factors in patients who are not candidates for conventional revascularization. The results of recent clinical trials of proangiogenesis gene therapy have been disappointing; however, significant limitations in experimental design, in particular in gene transfer strategies, preclude drawing definitive conclusions. In the REVASC study cardiac gene transfer was optimized by direct intramyocardial delivery of a replication-deficient adenovirus-containing vascular endothelial growth factor (AdVEGF121, 4 x 10(10) particle units (p.u.)). Sixty-seven patients with severe angina due to coronary artery disease and no conventional options for revascularization were randomized to AdVEGF121 gene transfer via mini-thoracotomy or continuation of maximal medical treatment. Exercise time to 1 mm ST-segment depression, the predefined primary end-point analysis, was significantly increased in the AdVEGF121 group compared to control at 26 weeks (P=0.026), but not at 12 weeks. As well, total exercise duration and time to moderate angina at weeks 12 and 26, and in angina symptoms as measured by the Canadian Cardiovascular Society Angina Class and Seattle Angina Questionnaire were all improved by VEGF gene transfer (all P-values at 12 and 26 weeks < or =0.001). However, if anything the results of nuclear perfusion imaging favored the control group, although the AdVEGF121 group achieved higher workloads. Overall there was no significant difference in adverse events between the two groups, despite the fact that procedure-related events were seen only in the thoracotomy group. Therefore, administration of AdVEGF121 by direct intramyocardial injections resulted in objective improvement in exercise-induced ischemia in patients with refractory ischemic heart disease.

Development and morphological variability of Echinococcus granulosus.

Characteristics are presented of Echinococcus granulosus strobila from dogs experimentally infected with protoscolices from a single cyst isolated from the liver of a domestic pig in a central Slovakian district. Adult cestodes are characterized mainly by 28-32 hooks highly variable in shape and size; 26-51 testes situated mostly throughout the proglottis (1 or 3 rows behind the vitelline gland); a lobate ovary; the shape of the uterus, which in 10% of proglottides is more or less spherical, in 62% is saccular with lateral sacculations of variable size, in 27% is saccular without lateral sacculations and in 1% is more or less tubular or strongly ramified; and an intermediately rapid rate of development lasting until between 40 and 44 days p.i. The present report analyzes the high degree of variability of the clone studied, referring to the knowledge on the characteristics of the pig, sheep, and cattle strains of E. granulosus and E. multilocularis species, with which some taxonomic characters of the clone representatives overlap.

On the problem of retarding definitive host (s.l.).

The significance of the species and individual level in determining the hosts as a category of helminth life cycles is pointed out. From the viewpoint of species level, the categories of intermediate host and definitive host are ascribed to certain organisms as representatives of a certain species on the basis of the fact that a respective stage of helminth life cycle can take place inside them. From the viewpoint of individual level, these types of hosts are determined on the basis of associations between the helminth individual and host individual. In order to avoid discrepancies, which might occur in determining the host type in these ways, it is proposed that the organism, which was determined as intermediate or definitive host at the species level, should be regarded identically also at the individual level. In addition, the existence of retarding definitive host (s.l.) is substantiated on the example of helminths which survived the interorgan migrations and passed to the offsprings through the intrauterine or transmammary route.

Deliberations on the problems of mammomonogamus species (Nematoda, Syngamidae) in ruminants.

The taxonomy of mammomonogamids parasitic in ruminants is discussed with regard to their different rates of variability in Cuba and Mexico. Since their variability is large and the existence of biological species is not based on their morphology but on the reproduction isolation of populations, the authors came to the conclusion that the specific analysis of mammomonogamids will be convincing only in case that it is carried out using taxonomic experiments of special orientation.

Population and classification of intrapopulation units of helminths.

The study of the population problems revealed that the population is an elementary evolution unit of a species with biparental way of reproduction. This is a general theoretical definition which the author elaborated in detail for the helminths. With regard to the complicated intraspecific structure of these parasites also the classification of intrapopulation helminth units is proposed.

On the topical problems of species in general and in relation to helminths.

In the light of the present knowledge of species, the author suggests to accept ten features of species and the general definition of species proposed by Zavadsky (1961, 1968), together with the definition by Mayr (1940) characterizing more concretely the species at the level of biparentally reproducing organisms. The definition by Timofeeff-Ressovsky et al. (1973) may be used for exclusively autogamous species of helminths. From the viewpoint of the species as a taxonomic unit, the paper presents proposals for establishment of a practical standard which would better suit the theoretical idea about the helminth species than it was the case till the present.

On some theoretical and practical aspects of population variability in helminths.

Possibilities of studies of some microevolutionary processes in helminths on the basis of knowledge of their variability are presented. The practical aspects of the study of helminth variability are also referred to. In conclusion the author states that the investigation of non-morphological characters of helminths, in relation to various morphological structures of phenotypes in the populations of parasite and host, represents a sphere in which the interests of theoretical and practical helminthology meet with one another.

Contribution to the knowledge of infectivity of Fasciola hepatica adolescariae after their passage through the digestive tract of white mice.

The study of infective elements of Fasciola hepatica which passed through the digestive tract of white mice revealed that those adolescariae (transitive adolescariae) (Table 1), which were not visibly damaged or possessed a more or less digested outer cyst wall, remained infective. The mice infected with transitive adolescariae harboured higher number of trematodes in the livers than those infected with infective elements which had not previously passed through the digestive tract of the definitive host.